We narrowed to 22,342 results for: Sis

1. pMarsL274G

   Plasmid

   #63177

   Purpose

   This plasmid encodes the mouse Mars gene with the L274G mutation under a CMV promoter.

   Depositor

   David Tirrell

   Article

   Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

   Insert

   MarsL274G (Mars1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L274G

   Promoter

   CMV

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)/AUG-DAP5-3XFLAG

   Plasmid

   #127331

   Purpose

   Express 3xFLAG tagged DAP5 protein from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   DAP5 (EIF4G2 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCRISPR v2 sgKEAP1-2

   Plasmid

   #186459

   Purpose

   knock out KEAP1 in mammalian cells

   Depositor

   Boyi Gan

   Article

   Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

   Insert

   Keap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. SunTagng14aa

   Plasmid

   #106439

   Purpose

   Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.

   Depositor

   Steven Jacobsen

   Article

   Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

   Insert

   NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN414aa_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_eA3A T31A-NG-nCas9

   Plasmid

   #163539

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A T31A-NG-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)eA3A T31ANG (L111R, D1135V, G1218R, E…

   Promoter

   Available since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLDLR-Luc mutSRE

   Plasmid

   #14945

   Depositor

   Axel Nohturfft

   Article

   Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Insert

   LDLR promoter mut SRE (LDLR Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Point mutation in the SRE-1 sequence of the LDL r…

   Promoter

   Available since

   May 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDuet-AtRBX1-T7-AtSKP1-T7-AtCUL1-S

   Plasmid

   #238003

   Purpose

   Expression AtRBX1-T7, AtSKP1-T7 and AtCUL1-S in in bacterial cells

   Depositor

   Lei Wu

   Article

   Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

   Inserts

   AtRBX1 (RBX1 Mustard Weed)
   AtSKP1 (SKP1 Mustard Weed)
   AtCUL1 (CUL1 Mustard Weed)
   

   Use

   Tags

   S and T7

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAP03-sp44/p21

   Plasmid

   #120232

   Purpose

   Carries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.

   Depositor

   Bradley Moore

   Article

   Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

   Insert

   bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   aac(3)IV (apramycin resistance gene)

   Promoter

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-Flag-mRIPK1-S321E

   Plasmid

   #115340

   Purpose

   Mammalian expression of Flag-mRIPK1-S321E

   Depositor

   Junying Yuan

   Article

   Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

   Insert

   RIPK1 (Ripk1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S321E

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. SunTag-FWAgRNA-4-22aa-TET1cd

    Plasmid

    #106435

    Purpose

    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

    Depositor

    Steven Jacobsen

    Article

    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)/GUG-DAP5-3XFLAG

    Plasmid

    #127330

    Purpose

    Express 3xFLAG tagged DAP5 protein from GUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    DAP5 (EIF4G2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. CRY-Gal∆DD (B1013)

    Plasmid

    #92035

    Purpose

    Expresses CRY2 (full-length, with endogenous NLS) fused to Gal4 (aa1-65 of Gal4BD), downstream of mCherry-IRES

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fusion of plant CRY2 to Gal4 binding domain (AA1-…

    Promoter

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. CIB-VP64 (B1016)

    Plasmid

    #92037

    Purpose

    Expresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CIB1-VP64 (CIB1 Mustard Weed)
    

    Use

    Tags

    Fusion of plant CIB1 with VP64 activation domain.…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-hPhyB-mCherry-HRasCT

    Plasmid

    #100281

    Purpose

    Expresses Phytochrome B (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Insert

    Phytochrome B (PHYB Mustard Weed)
    

    Use

    Tags

    mCherry fused with HRas C-terminus for plasma mem…

    Expression

    Mammalian

    Mutation

    Human codon optimized PhyB (1-908 aa)

    Promoter

    CAG promoter

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3/hArf5(WT)-mCherry

    Plasmid

    #79421

    Purpose

    Expresses C-terminally mCherry-tagged Arf5(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf5 (ARF5 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_UdgX-APOBEC1-UdgX-NG-nCas9

    Plasmid

    #163562

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-NG-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV_UdgX-Anc689-UdgX-NG-nCas9-RBMX

    Plasmid

    #163554

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-Anc689-UdgX-NG-nCas9-RBMX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Promoter

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiCRISPR v2 RB1 sgRNA #1

    Plasmid

    #202516

    Purpose

    Expressing Cas9 and sgRNA targeting human RB1 gene

    Depositor

    Ming Chen

    Article

    Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

    Insert

    RB1 sgRNA (RB1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9

    Plasmid

    #163561

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-HF-NG-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV5 HA-TBRII (delta Cyt)

    Plasmid

    #14051

    Depositor

    Joan Massague

    Article

    Siegel et al Proc Natl Acad Sci U S A. 2003 Jul 8. 10

    Insert

    TGF beta rec II (delta cyt) (TGFBR2 Human)
    

    Use

    Tags

    HA (PYDVPDYA)

    Expression

    Mammalian

    Mutation

    Dominant negative mutant. A stop codon was introd…

    Promoter

    Available since

    May 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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