We narrowed to 6,923 results for: pcdna3.1

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(344) pcDNA3.1-HA-nGFP-(EAAAK)4-OGA(544-706)

Plasmid

#168097

Purpose

HA- tagged GFP nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA.

Depositor

Christina Woo

Publication

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

HA-nGFP-(EAAAK)4-OGA(544-706)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-endo-GαsBP2 WA-mKate2

Plasmid

#250686

Purpose

Expressing endosome located GαsBP2 W11A inactive mutant

Depositor

Mikel Garcia-Marcos

Publication

Zhao et al Nat Chem Biol. 2026 Feb 3. doi: 10.1038/

Insert

endo-G_sBP2 W11A-mKate2

Tags

HA

Expression

Mammalian

Mutation

W11A in the G_sBP2 sequence

Promoter

CMV

Available since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-endo-GαsBP2 WT-mKate2

Plasmid

#250685

Purpose

Expressing endosome located GαsBP2 WT

Depositor

Mikel Garcia-Marcos

Publication

Zhao et al Nat Chem Biol. 2026 Feb 3. doi: 10.1038/

Insert

endo-G_sBP2 WT-mKate2

Tags

HA

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) Cystector (Cysteine biosensor)

Plasmid

#250653

Purpose

Cytosolic expression plasmid for Cystector (Cysteine biosensor) in mammalian cells.

Depositor

Peter Dedecker

Publication

Pedre et al bioRxiv 2026.01.27.698081

Insert

Cystector (Cysteine biosensor)

Expression

Mammalian

Promoter

CMV

Available since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab003-pcDNA3.1-FKBP5-3xFLAG

Plasmid

#249006

Purpose

This plasmid expresses the C terminally 3x-FLAG tagged FKBP5 protein sequence for pulldown assays

Depositor

Georgios Karras

Publication

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

FKBP5 (FKBP5 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Promoter

CMV Promoter

Available since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Flag-SOD1-G93A

Plasmid

#235662

Purpose

Express human SOD1-G93A in mammalian cells

Depositor

Chen Seng Ng

Publication

Hiew et al Commun Biol. 2025 Mar 2;8(1):347. doi: 1

Insert

SOD1-G93A (SOD1 Human)

Tags

Flag-tag at N-terminal

Expression

Mammalian

Mutation

changed Glycine 93 to alanine

Promoter

CMV promoter

Available since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloCKAR2.2-T2A-EGFP

Plasmid

#216493

Purpose

Expression of chemigenetic PKC biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Publication

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloCKAR2.2

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloAKAR2.1-T2A-EGFP

Plasmid

#216489

Purpose

Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Publication

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloAKAR2.1

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLT3-D835Y-V5/HIS

Plasmid

#236007

Purpose

expression of the D835Y kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia and Acute Lymphoblastic Leukemia

Depositor

Pavel Krejčí

Publication

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human FLT3-D835Y receptor tyrosine kinase, full length (FLT3 Human)

Tags

V5/His

Expression

Mammalian

Mutation

D835Y substitution

Promoter

CMV

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-Tau2N4R-WPRE

Plasmid

#226380

Purpose

Expresses tau propagation ORF with no V5 tag on WT 2N4R tau

Depositor

Bradley Hyman

Publication

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

TagRFP.T-2A

Expression

Mammalian

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-IDO2-p2a-eGFP

Plasmid

#187027

Purpose

Expresses IDO2 and GFP (seperately) in mammalian cells

Depositor

Geert van den Bogaart

Publication

Maassen et al Free Radic Biol Med. 2022 Aug 1;188:287-

Insert

Indoleamine 2,3-dioxygenase 2 (IDO2 Human)

Tags

P2A-GFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RIαΔ(D/D+linker)-EGFP

Plasmid

#181843

Purpose

GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.

Depositor

Jin Zhang

Publication

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

RIα-(D/D+linker)EGFP (PRKAR1A Human)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of residues 12-112 in PKA RIα.

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1(+)-cpPKN-mRuby2-GFP1-10

Plasmid

#181859

Purpose

Part of FluoSTEP-RhoA biosensor; must be expressed with GFP11-tagged RhoA plasmid (Addgene #181860) or in GFP11-RhoA gene-edited cells.

Depositor

Jin Zhang

Publication

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

cp-PKN-mRuby2-GFP(1-0)

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-mCherry-p115(S240A)

Plasmid

#181997

Purpose

Mammalian expression vector encoding S240A-mutated p115 RhoGEF tagged with mCherry.

Depositor

Jin Zhang

Publication

Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

Insert

mCherry-p115(S240A) (ARHGEF1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Serine 240 changed to alanine in p115.

Promoter

CMV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CCND3

Plasmid

#172623

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D3 in mammalian cells

Depositor

Michele Pagano

Publication

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1 6A NLRP1 CARD-FLAG WT

Plasmid

#163978

Purpose

Mammalian expression vector encoding human NLRP1 CARD with a C-terminal FLAG tag

Depositor

Hao Wu

Publication

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Available since

Jan. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_535-775 USP7

Plasmid

#131247

Purpose

Mammalian expression of the USP7 UBL1-2 domains with an N-terminal Myc tag.

Depositor

Roger Woodgate

Publication

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_775-1102 USP7

Plasmid

#131250

Purpose

Mammalian expression of the USP7 UBL3-5 domains with an N-terminal Myc tag

Depositor

Roger Woodgate

Publication

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Encodes USP7 UBL domains 3-5 (USP7 amino acids 77…

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_1-1083 USP7

Plasmid

#131251

Purpose

Mammalian expression of N-terminally Myc-tagged USP7, minus the C-terminal 18 amino acids

Depositor

Roger Woodgate

Publication

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

A mutant form of our pcDNA3.1-N-Myc_WT USP7 plasm…

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GFP-2AFP-KDEL

Plasmid

#154349

Purpose

Mammalian expression of 2AFP

Depositor

Wanzhong He

Publication

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

2AFP

Tags

GFP

Expression

Mammalian

Available since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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