162,717 results

1. pCS2 Notch1 Full Length-6MT

   Plasmid

   #41728

   Depositor

   Raphael Kopan

   Article

   Schroeter et al Nature. 1998 May 28;393(6683):382-6.

   Insert

   Notch-1 Full Length (Notch1 Mouse)
   

   Use

   Tags

   6xMyc

   Expression

   Mammalian

   Mutation

   L1997V; Contains murine Notch-1 protein up to ami…

   Promoter

   Available since

   Dec. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TAC-ERP2

   Plasmid

   #80478

   Purpose

   All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)

   Depositor

   Knut Woltjen

   Article

   Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-FRB-VMAc-CSarcin

   Plasmid

   #70223

   Purpose

   Expresses VMA intein with C-terminal portion of alpha-sarcin

   Depositor

   Perry Howard

   Article

   Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

   Insert

   alpha Sarcin (C-terminal portion)
   

   Use

   Tags

   FKBP-rapamycin binding (FRB) domain of the mammal…

   Expression

   Mammalian

   Mutation

   Changed Asp 28 to Cys, deleted amino acids 1-27

   Promoter

   CMV

   Available since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

4. Perox-GA

   Plasmid

   #209868

   Purpose

   Dimerization dependent fluorescent protein GA anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP A
   

   Use

   Tags

   Targeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE EF1α dCas9-NED

   Plasmid

   #109369

   Purpose

   Lentiviral vector for expressing effector domains fused to dCas9.

   Depositor

   Antal Kiss

   Article

   Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.4_StrepII_TEV_PP2Ac

   Plasmid

   #217477

   Purpose

   Expresses PP2Ac with a TEV cleavable N-terminal StrepII tag

   Depositor

   Wolfgang Peti

   Article

   Padi et al Nature. 2024 Jan;625(7993):195-203. doi:

   Insert

   PP2Ac
   

   Use

   Tags

   StrepII, TEV

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. NSarQ27G-VMAn-FKBP

   Plasmid

   #70230

   Purpose

   Expresses N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

   Depositor

   Perry Howard

   Article

   Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

   Insert

   alpha Sarcin (N-terminal portion)
   

   Use

   Tags

   FK506- and rapamycin-binding protein (FKBP), Link…

   Expression

   Mammalian

   Mutation

   Changed Gln 27 to Gly

   Promoter

   CMV

   Available since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pCAP05

   Plasmid

   #102920

   Purpose

   Direct cloning and broad-host-range heterologous expression vector

   Depositor

   Bradley Moore

   Article

   Zhang et al MBio. 2017 Sep 5;8(5). pii: e01291-17. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS6-YFP-SMASh

   Plasmid

   #68853

   Purpose

   Expresses SMASh tag fused to YFP at C terminus in mammalian cells

   Depositor

   Michael Lin

   Article

   Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

   Insert

   YFP-SMASh
   

   Use

   Tags

   SMASh tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MiCy-pBAD

    Plasmid

    #54565

    Purpose

    Localization: Protein Expression Vector , Excitation: 472, Emission: 495

    Depositor

    Michael Davidson, Atsushi Miyawaki

    Article

    Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

    Type

    Empty backbone

    Use

    Tags

    6xHis and MiCy

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only