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pPGK-T7/2-CD44v6-10 (fl)

Plasmid

#137820

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v6-10 (fl) (CD44 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

full length

Promoter

PGK

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44v6 (fl)

Plasmid

#137816

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v6 (fl) (CD44 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

R296K in the V6 region [Please see depositor comm…

Promoter

PGK

Available since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3

Plasmid

#170163

Purpose

Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

ChroME2s-P2A-H2B-mRuby3

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-PCAF

Plasmid

#63705

Purpose

retroviral expression of human PCAF in mammalian cells

Depositor

Kai Ge

Article

Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

Insert

PCAF (KAT2B Human)

Use

Retroviral

Tags

None

Expression

Mammalian

Mutation

E452G

Promoter

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-LATS2

Plasmid

#166474

Purpose

Expresses fusion of mEGFP and LATS2

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, LATS2 (LATS2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLT 651

Plasmid

#59998

Purpose

Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans klp-16/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny

Depositor

Lisa Timmons

Article

Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(

Inserts

klp-16 (klp-16 Nematode)
him-8 (him-8 Nematode)

Use

Tags

Expression

Bacterial

Mutation

partial genomic

Promoter

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44v8-10 (fl)

Plasmid

#137823

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v8-10 (fl) (CD44 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

full length

Promoter

PGK

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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p21-WT-MEFV

Plasmid

#134702

Purpose

Lentiviral expression of human MEFV after doxycycline administration

Depositor

Thomas Henry

Article

Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

Insert

MEFV (MEFV Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mutation

WT

Promoter

TRE2 (Dox-inducible)

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pNK3203

Plasmid

#219749

Purpose

DVK_AF CIDAR vector encoding hispidin-synthase from Mycena citricolor under control of CMV promoter, for mammalian expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pCMV - mcitHispS - tSV40

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Gcn5

Plasmid

#63706

Purpose

retroviral expression of mouse Gcn5 in mammalian cells

Depositor

Kai Ge

Article

Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

Insert

Gcn5 (Kat2a Mouse)

Use

Retroviral

Tags

None

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
pCyCEN_Lisp2mCherry_hsp70_GFP

Plasmid

#137169

Purpose

Dual fluorescent reporter for liver stage expression in Plasmodium cynomolgi

Depositor

Clemens Kocken

Article

Voorberg-van der Wel et al Commun Biol. 2020 Jan 3;3:7. doi: 10.103

Inserts

mCherry
Nanoluc
dihydrofolate reductase
Green Fluorescent Protein

Use

Unspecified

Tags

T2A

Expression

Mutation

Promoter

P. cynomolgi hsp70 and P. cynomolgi lisp2

Available since

May 11, 2020

Availability

Academic Institutions and Nonprofits only
pBW2614_pCAG-PV1-GAI-L2-28-396-FlpO-ABI-NLS-BGHpA

Plasmid

#108833

Purpose

Expresses aa28 to aa396 of Flp recombinase fused to GAI and ABI CIDs

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa28 to aa396

Use

Cre/Lox and Synthetic Biology

Tags

ABI,NLS and GAI

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44v3 (fl)

Plasmid

#137814

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v3 (fl) (CD44 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

full length and N280D in the V3 region

Promoter

PGK

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-FKBP-STING(pLxIS)

Plasmid

#221333

Purpose

Overexpression of STING CTT in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

STING CTT (Sting1 Mouse)

Use

Tags

6xHis-4xFKBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCLXSN-Tax

Plasmid

#44038

Depositor

Shao-Cong Sun

Article

Rivera-Walsh et al J Biol Chem. 2001 Nov 2;276(44):40385-8.

Insert

5'UTR-Tax-3'UTR (CNTN2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-DIO-ChroME-ST-P2A-H2B-mRuby3

Plasmid

#170161

Purpose

Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

ChroME-P2A-H2B-mRuby3

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-MST2

Plasmid

#166475

Purpose

Expresses fusion of mEGFP and MST2

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, MST2 (STK3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEXPqcxip-hCCDC47-FLAG

Plasmid

#159141

Purpose

Hu CCDC47 -flag mammalian expression Gateway vector.

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

coiled-coil domain containing 47 (CCDC47 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV (cytomegalovirus) enhancer and the MSV (mouse…

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCAX APPs-751-alpha

Plasmid

#30149

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein - soluble (APP Human)

Use

Tags

Expression

Mammalian

Mutation

splice variant 751; amino acids 1–612

Promoter

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-Cdt1 human

Plasmid

#72681

Purpose

mammalian expression of human Cdt1

Depositor

Domenico Maiorano

Article

Tsanov et al Nucleic Acids Res. 2014 Apr;42(6):3692-7

Insert

Cdt1 (CDT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only