We narrowed to 9,256 results for: SUB

1. pRS3 (CK2alpha, CK2beta)

   Plasmid

   #27092

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Inserts

   CK2beta (CSNK2B Human)
   CK2alpha (CSNK2A1 Human)
   

   Use

   Tetracycline-regulated expression

   Tags

   HA and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   bidirectional tet-responsive promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TRIPZ-bioGEF-UBnc-PURO

   Plasmid

   #208044

   Purpose

   Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   UBnc (UBC Human)
   

   Use

   Lentiviral

   Tags

   Low-affinity Avitag

   Expression

   Mutation

   L73P Mutation near C-terminus suppresses cleavage…

   Promoter

   tetO/UBC

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR223_PER1_WT

   Plasmid

   #82152

   Purpose

   Gateway Donor vector containing PER1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   PER1 (PER1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDONR223_ATG16L1_WT

   Plasmid

   #82255

   Purpose

   Gateway Donor vector containing ATG16L1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   ATG16L1 (ATG16L1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONR223_BRAF_p.V600E

   Plasmid

   #81700

   Purpose

   Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   BRAF (BRAF Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   V600E

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONR223_PIK3CA_p.E545K

   Plasmid

   #82881

   Purpose

   Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Matthew Meyerson, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   NM_006218 (PIK3CA Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   E545K

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR223_NRAS_p.Q61R

   Plasmid

   #81652

   Purpose

   Gateway Donor vector containing NRAS, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   NRAS (NRAS Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Q61R

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-FLEX-P301L Tau

   Plasmid

   #176267

   Purpose

   This AAV plasmid vector expresses human 2N4R microtubule-associated protein tau with P301L mutation.

   Depositor

   Tsuneya Ikezu

   Article

   You et al Neuroscience. 2019 Dec 1;422:65-74. doi:

   Insert

   Flex-P301L tau (MAPT )
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Anti-DLX1 [L43/40R]

   Plasmid

   #128633

   Purpose

   Mammalian Expression Plasmid of anti-DLX1 (Human). Derived from hybridoma L43/40.

   Depositor

   James Trimmer

   Article

   Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   anti-DLX1 (Human) recombinant mouse monoclonal antibody (DLX1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR223_RHOA_WT

    Plasmid

    #81739

    Purpose

    Gateway Donor vector containing RHOA , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RHOA (RHOA Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mCherry-APPP1-mGFP

    Plasmid

    #196705

    Purpose

    As mCherry-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    mCherry and mEGFP

    Expression

    Mammalian

    Mutation

    Lys to Val substitution at position 612

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-N1_c-SRC

    Plasmid

    #171850

    Purpose

    pEGFP-N1_c-SRC

    Depositor

    Michele Pagano

    Article

    Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

    Insert

    c-SRC (SRC Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Added a linker sequence in 3' of c-SRC: GSGS

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pML333_LAMP1-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227898

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    LAMP1 homology arms with mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pML334_RAB7A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227899

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    RAB7A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR223_CTNNB1_WT

    Plasmid

    #81734

    Purpose

    Gateway Donor vector containing CTNNB1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CTNNB1 (CTNNB1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Anti-GABA(A)R, Epsilon [N413/67R]

    Plasmid

    #114478

    Purpose

    Mammalian Expression Plasmid of anti-GABA(A)R, Epsilon (Human). Derived from hybridoma N413/67.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-GABA(A)R, Epsilon (Homo sapiens) recombinant mouse monoclonal antibody (GABRE Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Anti-GABA(A)R, Delta [N151/3.3R]

    Plasmid

    #114515

    Purpose

    Mammalian Expression Plasmid of anti-GABA(A)R, Delta (Rat). Derived from hybridoma N151/3.3.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-GABA(A)R, Delta (Rattus norvegicus) recombinant mouse monoclonal antibody (Gabrd Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Anti-GABA(A)R, alpha6 [N229A/32R]

    Plasmid

    #164155

    Purpose

    Mammalian Expression Plasmid of anti-GABA(A)R, alpha6 (Mouse). Derived from hybridoma N229A/32.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-GABA(A)R, alpha6 (Mus musculus) recombinant mouse monoclonal antibody (Gabra6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Anti-GABA(A)R, Alpha5 [N415/24R]

    Plasmid

    #149456

    Purpose

    Mammalian Expression Plasmid of anti-GABA(A)R, Alpha5 (Human). Derived from hybridoma N415/24.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-GABA(A)R, Alpha5 (Homo sapiens) recombinant mouse monoclonal antibody (GABRA5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Anti-GABA(A)R, Beta1 [N96/55.3R]

    Plasmid

    #114549

    Purpose

    Mammalian Expression Plasmid of anti-GABA(A)R, Beta1 (Mouse). Derived from hybridoma N96/55.3.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-GABA(A)R, Beta1 (Mus musculus) recombinant mouse monoclonal antibody (Gabra1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pML531_TOMM20-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227890

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    TOMM20 homology arms with mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDONR223_CTNNB1_p.S45F

    Plasmid

    #82821

    Purpose

    Gateway Donor vector containing CTNNB1, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Matthew Meyerson, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    CTNNB1 (CTNNB1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    S45F

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDONR223_EGFR_p.T790M_p.L858R

    Plasmid

    #82914

    Purpose

    Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Matthew Meyerson, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    EGFR (EGFR Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    T790M, L858R

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDFDuet-MKK6-EE p38alpha

    Plasmid

    #82719

    Purpose

    Expresses constitutively active human MKK6 and murine p38 alpha.

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Inserts

    MKK6 (MAP2K6 Human)
    p38alpha (Mapk14 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Ser207 to Glu, Thr211 to Glu

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

25. pDONR223_NRAS_WT

    Plasmid

    #82151

    Purpose

    Gateway Donor vector containing NRAS , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    NRAS (NRAS Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-kappa-myc-dL5-2xG4S-TMst

    Plasmid

    #73206

    Purpose

    Expresses myc-dL5(E52D)-TM (PDGFR derived) on the surface of mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

    Insert

    kappa-myc-dL5-2XG4S-TM (MYC Human)
    

    Use

    Tags

    The FAP is fused with 2 copies of a G4S linker an…

    Expression

    Mammalian

    Mutation

    The dL5** FAP is E50D, L89S, also known as E52D/L…

    Promoter

    CMV

    Available since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mBFP-APPP1-mGFP

    Plasmid

    #196695

    Purpose

    As mBFP-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    mEGFP and mtagBFP2

    Expression

    Mammalian

    Mutation

    Lys to Val substitution at position 612

    Promoter

    CMV

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDONR223_ATF2_WT

    Plasmid

    #82889

    Purpose

    Gateway Donor vector containing ATF2, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Matthew Meyerson, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    ATF2 (ATF2 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDONR223_RHOA_p.Y42C

    Plasmid

    #81306

    Purpose

    Gateway Donor vector containing RHOA , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RHOA (RHOA Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Y42C

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONR223_TGFBR1_WT

    Plasmid

    #82237

    Purpose

    Gateway Donor vector containing TGFBR1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    TGFBR1 (TGFB1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDONR223_WISP2_WT

    Plasmid

    #81905

    Purpose

    Gateway Donor vector containing WISP2 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    WISP2 (CCN5 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDONR223_CTCF_WT

    Plasmid

    #81789

    Purpose

    Gateway Donor vector containing CTCF , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CTCF (CTCF Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLEX307-mFzd5

    Plasmid

    #102867

    Purpose

    Expresses mouse Fzd5 in mammalian cells

    Depositor

    Henry Ho

    Article

    Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

    Insert

    Frizzled 5 (Fzd5 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    A to G substitution at nucleotide 699 of Fzd5 ORF…

    Promoter

    EF1A

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDONR223_STAT3_WT

    Plasmid

    #82235

    Purpose

    Gateway Donor vector containing STAT3, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDONR223_HRAS_WT

    Plasmid

    #81777

    Purpose

    Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    HRAS (HRAS Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDONR223_BRD4_WT

    Plasmid

    #82323

    Purpose

    Gateway Donor vector containing BRD4 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    P474L

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDONR223_NOTCH1_ICN

    Plasmid

    #82087

    Purpose

    Gateway Donor vector containing NOTCH1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    NOTCH1 (NOTCH1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    ICN (AA1761-2556 present)

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEGBacmam-NGS Wnt-Fc

    Plasmid

    #159629

    Purpose

    Express Fc-tagged Fzd7/8 subtype NGS Wnt in mammalian cells for organoid research

    Depositor

    Chris Garcia

    Article

    Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

    Insert

    Fzd7/8 subtype NGS Wnt Fc
    

    Use

    Tags

    Fc tag and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDONR223_EGFR_p.L858R

    Plasmid

    #82906

    Purpose

    Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Matthew Meyerson, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    EGFR (EGFR Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    L858R

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAB1923 REPAIR.t1-S

    Plasmid

    #176321

    Purpose

    CMV-HIVNES-GS-dCas13bt1- (GGS)2- huADAR2dd(E488Q/E620G/Q696L) (REPAIR.t1-S expression, specificity enhancing mutations)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Inserts

    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Mutation

    E488Q/E620G/Q696L, only the deaminase domain (aa …

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pDONR207 SARS-CoV-2 NSP1 Delta RC

    Plasmid

    #164522

    Purpose

    Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions K164A/H165A)

    Depositor

    Mitchell Guttman

    Article

    Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

    Insert

    NSP1 Delta RC (ORF1ab )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Insert of NSP1 gene's CDS from SARS-CoV-2 is…

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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42. pDONR223_MYC_WT

    Plasmid

    #82927

    Purpose

    Gateway Donor vector containing MYC, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Matthew Meyerson, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    Myc (MYC Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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43. pDONR223_AKR1B1_WT_V5

    Plasmid

    #82953

    Purpose

    Gateway Donor vector containing AKR1B1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Matthew Meyerson, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    AKR1B1 (AKR1B1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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44. pCDNA3 Flag MKK7B2Jnk1a1

    Plasmid

    #19726

    Depositor

    Roger Davis

    Article

    Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

    Insert

    MKK7B2Jnk1a1 (Map2k7, MAPK8 Mouse, Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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45. Tet-O-FUW-KDM2B-P2A-mCherry

    Plasmid

    #203852

    Purpose

    Tet-inducible lentiviral plasmid expressing KDM2B-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    KDM2B (KDM2B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. pcDNA3-MKK1K97A

    Plasmid

    #107974

    Purpose

    expression of a dominant negative MAP Kinase Kinase

    Depositor

    Edwin Krebs

    Article

    Seger et al J Biol Chem. 1994 Oct 14;269(41):25699-7

    Insert

    MKK1 K97A (MAP2K1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dominant negative; see Depositor Comments below

    Promoter

    CMV

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. pDONR223_SRC_WT

    Plasmid

    #82165

    Purpose

    Gateway Donor vector containing SRC , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SRC (SRC Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pDONR223_H2AFX_WT

    Plasmid

    #81937

    Purpose

    Gateway Donor vector containing H2AFX, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    H2AFX (H2AX Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. pDONR223_GSK3B_WT

    Plasmid

    #82126

    Purpose

    Gateway Donor vector containing GSK3B , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    GSK3B (GSK3B Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. pDONR223_ETS1_WT

    Plasmid

    #82118

    Purpose

    Gateway Donor vector containing ETS1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    ETS1 (ETS1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge