We narrowed to 3,367 results for: aaas

1. lentiCRISPR - C3orf17 sgRNA 2

   Plasmid

   #70653

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against C3orf17
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pLL3.7 Lmo3.5 shRNA

   Plasmid

   #59295

   Purpose

   shRNA to mouse Lmo3

   Depositor

   Ulrike Heberlein

   Article

   Lasek et al Alcohol Clin Exp Res. 2011 Sep;35(9):160

   Insert

   Lmo3.5 shRNA
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

3. MXW-PGK-c-let-7-LP3-IRES-EGFP

   Plasmid

   #37642

   Depositor

   Chang-Zheng Chen

   Article

   Yue et al RNA Biol. 2011 Nov-Dec;8(6):1115-23.

   Insert

   cel-let-7
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   LP3 - AATATTACCA=>AAATTTACCA

   Promoter

   mPGK

   Available Since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PCV-Cas9

   Plasmid

   #123643

   Purpose

   Expresses Cas9 with an N-terminal HUH-tag called PCV2

   Depositor

   Wendy Gordon

   Article

   Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

   Inserts

   PCV2 (ORF1 )
   Cas9
   

   Tags

   (EAAAK)8 linker and His6 + SUMO

   Expression

   Bacterial

   Mutation

   2-116

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTRIPZ-shWT1_B

   Plasmid

   #217637

   Purpose

   Dox inducible shRNA targeting WT-1

   Depositor

   Constanze Bonifer

   Article

   Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

   Insert

   shWT1_B (WT1 Human)
   

   Use

   RNAi

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHS2002

   Plasmid

   #239832

   Purpose

   Engineered OrufIscB (NovaIscB) omegaRNA mammalian expression

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

   Insert

   NovaIscB omegaRNA scaffold
   

   Expression

   Mammalian

   Mutation

   Replacement of A12-A57 with GAAA in omegaRNA scaf…

   Promoter

   human U6

   Available Since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLVX-shRNA2-zsGreen-PGK-puro_scramble shRNA

   Plasmid

   #197991

   Purpose

   For the stable knockdown experiments using shRNA, scramble sequence was inserted as the control.

   Depositor

   Bo Wang

   Article

   Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

   Insert

   scramble shRNA (control)
   

   Use

   Lentiviral

   Tags

   zsGreen1

   Expression

   Mammalian

   Mutation

   15q11.2 deletion

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcw107

   Plasmid

   #62511

   Purpose

   Gateway Expression Vector

   Depositor

   John Doench, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAH750

   Plasmid

   #91212

   Purpose

   protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtSL7 promoters, structurally optimized gRNA scaffolds)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   2 gRNAs targeting tomato ARF8A
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. phage UBC NLS-HA-2XMCP-tagRFPt

    Plasmid

    #64541

    Purpose

    Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to tagRFP.

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    MCP
    

    Use

    Lentiviral

    Tags

    HA, NLS, and tagRFP-T

    Expression

    Mammalian

    Promoter

    human ubiquitin C promoter

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFUChW Parkin shRNA Mul1 shRNA

    Plasmid

    #78523

    Purpose

    Lentiviral vector encoding two shRNAs and mCherry reporter

    Depositor

    David Chan

    Article

    Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

    Insert

    Park2 shRNA, Mul1 shRNA (Prkn, Mul1 Mouse)
    

    Use

    Lentiviral and RNAi

    Promoter

    H1

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. phage ubc nls ha pcp gfp

    Plasmid

    #64539

    Purpose

    Used to label reporter mRNAs. Lentiviral expression of the PP7 coat protein fused to EGFP.

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    PCP
    

    Use

    Lentiviral

    Tags

    EGFP, FactorXa site, HA, and NLS

    Expression

    Mammalian

    Promoter

    human ubiquitin C promoter

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBBK19 pCAS-Tyr-[gRNA: 2=FF21] (SplitHygR, AmpR)

    Plasmid

    #179003

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDECKO_GFP

    Plasmid

    #72619

    Purpose

    Expresses two gRNAs targeting GFP

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward GFP
    

    Expression

    Mammalian

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHsp83-NLS-HA-2xMCP-2xTagRFP-T

    Plasmid

    #71242

    Purpose

    Used to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T.

    Depositor

    Anne Ephrussi

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    NLS-2xMCP-2xTagRFP-T
    

    Use

    P element-mediated germline transformation

    Tags

    TagRFP-T

    Expression

    Insect

    Promoter

    Hsp83

    Available Since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. phage ubc nls ha 2xmcp HALO

    Plasmid

    #64540

    Purpose

    Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to HALO.

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    MCP
    

    Use

    Lentiviral

    Tags

    HA, HALO, and NLS

    Expression

    Mammalian

    Promoter

    human ubiquitin C promoter

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQCascade

    Plasmid

    #140622

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and CRISPR RNA

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMD19-PBBa_J23117-sgRNA-Spr

    Plasmid

    #190793

    Purpose

    Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Insert

    sgRNA (dummy)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BBa_J23117

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHsp83-NLS-HA-2xPCP-2xGFP

    Plasmid

    #71243

    Purpose

    Used to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP.

    Depositor

    Anne Ephrussi

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    2xPCP-2xGFP
    

    Use

    P element-mediated germline transformation

    Tags

    EGFP

    Expression

    Insect

    Promoter

    Hsp83

    Available Since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p-AAV-sh[SNCA]

    Plasmid

    #75437

    Purpose

    knockdown alpha-synuclein in rat neurons

    Depositor

    Edward Burton

    Article

    Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

    Insert

    snca (Snca Rat)
    

    Use

    AAV and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge