We narrowed to 4,048 results for: gast

1. pKC50

   Plasmid

   #226778

   Purpose

   Expresses Drosophila UNC-104(V6M) in insect cells

   Depositor

   Kyoko Chiba

   Article

   Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

   Insert

   Drosophila unc-104 (V6M) (unc-104 Fly)
   

   Use

   Tags

   sfGFP and 2xStrepII

   Expression

   Insect

   Mutation

   changed Valine 6 to Methionine

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKC28

   Plasmid

   #226776

   Purpose

   Expresses Drosophila UNC-104(G618R) in insect cells

   Depositor

   Kyoko Chiba

   Article

   Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

   Insert

   Drosophila unc-104 (G618R) (unc-104 Fly)
   

   Use

   Tags

   sfGFP and 2xStrepII

   Expression

   Insect

   Mutation

   G618R

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Hy_pMT Laccase2 MCS Exon Vector

   Plasmid

   #69884

   Purpose

   Expression plasmid for expressing circular RNAs of a desired sequence in Drosophila

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   Laccase2 (stw Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBP-split-intein-Gal4[1-20]-N-int

   Plasmid

   #199201

   Purpose

   Gateway destination vector for enhancer-driven split-intein Gal4[1-20]N-int expression

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   split-intein-Gal4[1-20]-N-int
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBS-KS-attB1-2-PT-SA-SD-1-GFP11

   Plasmid

   #172068

   Purpose

   Protein trap plasmid for inserting GFP11 into a phase 1 coding intron

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   GFP11x7
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

   Plasmid

   #63168

   Purpose

   GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "spaghetti monster GFP-V5"
   

   Use

   Tags

   N-myristoylation

   Expression

   Insect

   Mutation

   Promoter

   GAL4-responsive UAS hsp70

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc

   Plasmid

   #63167

   Purpose

   GAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "spaghetti monster GFP-cMyc"
   

   Use

   Tags

   N-myristoylation

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG

   Plasmid

   #63169

   Purpose

   GAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "spaghetti monster GFP-FLAG"
   

   Use

   Tags

   N-myristoylation

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMT-Hrp48-ADARcd-E488Q-V5

   Plasmid

   #139685

   Purpose

   Expresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assays

   Depositor

   Michael Rosbash

   Article

   Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

   Insert

   Hrp48, ADAR (Adar Fly)
   

   Use

   Tags

   V5

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUAST td tomato Rab5 WT

    Plasmid

    #58446

    Purpose

    expression of drosophila td tomato Rab5

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab5 (Rab5 Fly)
    

    Use

    Tags

    td tomato

    Expression

    Insect

    Mutation

    Promoter

    GAL4

    Available since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

11. 10XSTAT92E–luciferase

    Plasmid

    #37393

    Depositor

    Norbert Perrimon

    Article

    Baeg et al Genes Dev. 2005 Aug 15;19(16):1861-70. E

    Insert

    SOCS36E enhancer fragment (Socs36E Fly)
    

    Use

    Luciferase; Jak/stat reporter construct

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Hsp70

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. doraWT_rescue_construct

    Plasmid

    #190609

    Purpose

    Puromycin-selectable expression of Dora (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUASTattB_NSlmb-vhhGFP4

    Plasmid

    #35577

    Depositor

    Markus Affolter

    Article

    Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

    Insert

    NSlmb-vhhGFP4 (slmb Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCFJ1209

    Plasmid

    #44491

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, NeoR]
    

    Use

    Gateway expression clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUAST_NSlmb-vhhGFP4

    Plasmid

    #35575

    Depositor

    Markus Affolter

    Article

    Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

    Insert

    NSlmb-vhhGFP4 (slmb Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)

    Plasmid

    #172066

    Purpose

    Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    mTagBFP2(11x7)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pRB17

    Plasmid

    #52527

    Purpose

    serves as template for PCR-mediated fusion of U6-promoter with sgRNA

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    Drosophila U6C promoter
    

    Use

    CRISPR; Blunt cloning kit

    Tags

    T7-promoter

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRmHA3-CH2F2-CG9866

    Plasmid

    #52286

    Purpose

    Expresses Drosophila Ataxin-7

    Depositor

    Jerry Workman

    Article

    Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

    Insert

    Ataxin-7 (CG9866 Fly)
    

    Use

    Tags

    2xHA 2xFLAG

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)

    Plasmid

    #172065

    Purpose

    Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    mTagBFP2(11x7)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pME18ST-d5-HT7

    Plasmid

    #53111

    Purpose

    Expression of Drosophila 5-HT7

    Depositor

    Leslie Vosshall

    Article

    Gasque et al Sci Rep. 2013;3:srep02120. doi: 10.1038/

    Insert

    5HT7 (5-HT7 Fly)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only