We narrowed to 3,319 results for: gfp lenti

1. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(PAMmut)

   Plasmid

   #176139

   Purpose

   EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   XRCC1-T2A-PolB (XRCC1, POLB Human)
   

   Use

   Lentiviral

   Tags

   EGFP and MYC

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. phage - ubc - nls - 2xmcp - egfp - cMycBirA*

   Plasmid

   #131133

   Purpose

   BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

   Depositor

   Ralf Jansen

   Insert

   NLS-HA-2xMCP-EGFP-cMycBirA*
   

   Use

   Lentiviral

   Tags

   NLS, HA, cMyc (N-terminal of BirA*)

   Expression

   Mammalian

   Mutation

   BirA* = BIrA >R118G

   Promoter

   human ubiquitin C (UBC)

   Available since

   Aug. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE pGK LysoFlux (HaloTag7-mGFP-Galectin3)

   Plasmid

   #215009

   Purpose

   Express a Lysophagy reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   LGALS3 (LGALS3 Human)
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE

   Plasmid

   #89609

   Purpose

   over-expression of VEGF-A(165) in human cells

   Depositor

   Fernando Fierro

   Insert

   VEGF-A(165) (VEGFA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   K188E

   Promoter

   MNDU3

   Available since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV UBC hTMEM115-3C-3xHA-P2A-eGFP

   Plasmid

   #214996

   Purpose

   Stably express the GolgiTag (GolgiIP handle) in mammalian cells (human ORF). eGFP for cell sorting desired expression levels.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   TMEM115 (TMEM115 Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV:eGFP-p2A-HA-β2Ar-uTEV1Δ(220-242)

   Plasmid

   #135459

   Purpose

   Mammalian expression of SPARK protease component

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   eGFP-p2A-HA-βarrestin2-uTEV1Δ
   

   Use

   Lentiviral

   Tags

   eGFP, HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV UBC msTMEM115-3C-3xHA-P2A-eGFP

   Plasmid

   #214997

   Purpose

   Stably express the GolgiTag (GolgiIP handle) in mammalian cells (mouse ORF). eGFP for cell sorting desired expression levels.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   TMEM115 (Tmem115 Mouse)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV UBC APEX2-3xFLAG-IRES-eGFP

   Plasmid

   #214983

   Purpose

   Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   APEX2 (APX2 Glycine max (soybean))
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   UBC

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)

   Plasmid

   #31476

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   SFFV d14 GFP donor EF1s I-SceI
   

   Use

   Lentiviral

   Tags

   HA and NLS

   Expression

   Mutation

   GFP has 14 amino acids deleted from c terminus

   Promoter

   Available since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-scFv-GCN4-sfGFP-GB1-dWPRE

    Plasmid

    #60907

    Purpose

    The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imaging

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    scFv-GCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin

    Plasmid

    #134858

    Purpose

    Expresses EGFP-tagged early endosome marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Ras-related protein 5A
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP

    Plasmid

    #128424

    Purpose

    Human Codon Optimized dCas9-DNMT3A3L

    Depositor

    Bradley Bernstein

    Article

    Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

    Insert

    dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7 Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin

    Plasmid

    #134662

    Purpose

    Expresses EGFP-tagged plasma membrane marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    vesicle-associated membrane protein 5 (VAMP5 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nls

    Plasmid

    #64113

    Purpose

    Expression of St1 dCas9-3XGFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-EF1a-EGFP-RAB3C-IRES-Puromycin

    Plasmid

    #134857

    Purpose

    Expresses EGFP-tagged synaptic vesicle marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Ras-related protein 3C
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHAGE-TO-nls-st1dCas9-3nls-3XGFP-nls

    Plasmid

    #64112

    Purpose

    Expression of St1 dCas9-3XGFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBS NL4-3 envFS eGFP RT-D185K/D186L

    Plasmid

    #101332

    Purpose

    pBS NL4-3 envFS eGFP with mutation that disrupts RT catalytic activity

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 envFS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    reverse transcriptase D185K/D186L

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMOS010: CheRiff-eGFP channelrhodopsin (plasma membrane)

    Plasmid

    #163044

    Purpose

    CheRiff-eGFP channelrhodopsin (plasma membrane)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    CheRiff
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pALPS_3'LTR GFP@gag start SFFV-

    Plasmid

    #101337

    Purpose

    Repaired U3 allows LTR-based transcription by the provirus with GFP as a marker for expression. WPRE was deleted.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBS NL4-3 envFS eGFP Δgag/pol

    Plasmid

    #101343

    Purpose

    Deletion from the start of gag until 229 bases before the cPPT

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    delta GagPol

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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