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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,673 results for: pcr

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Showing: 2081 - 2100 of 4673 results

  2. RXFP2-Tango

    Plasmid
    #66493
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RXFP2 (RXFP2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66493-plasmid-map-sequence-id-310431

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  3. LPAR4-Tango

    Plasmid
    #66420
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LPA4 (LPAR4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  4. CCR3-Tango

    Plasmid
    #66240
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CCR3 (CCR3 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66240-plasmid-map-sequence-id-314158

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  5. HRH4-Tango

    Plasmid
    #66403
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HRH4 (HRH4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. Cer(1-158)-beta-5 in pcDNAI/Amp

    Plasmid
    #55708
    Purpose
    An amino-terminal fragment of mCerulean was fused to Gbeta5. When co-expressed with a carboxyl terminal CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Publication
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCer(1-158)-beta5 (GNB5 Aequorea victoria, Human)
    Tags
    Cer(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    GBeta-5 was amplified via PCR, which added an N-t…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55708-plasmid-map-sequence-id-114838

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  7. YFP(1-158)-gamma-2 in pcDNAI/Amp

    Plasmid
    #55762
    Purpose
    An amino-terminal YFP fragment was fused to Ggamma2. When co-expressed with a carboxyl terminal YFP or CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Publication
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    YFP(1-158)-gamma-2 (GNG2 Aequorea victoria, Human)
    Tags
    YFP(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    YFP (1-158) includes a substitution of Met for Gl…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55762-plasmid-map-sequence-id-115200

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  8. YFP(159-238)-gamma-10 in pcDNAI/Amp

    Plasmid
    #55192
    Purpose
    A carboxyl-terminal YFP fragment was fused to Ggamma-10. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Publication
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    YFP-(159-238)-gamma-10 (GNG10 Aequorea victoria, Human)
    Tags
    YFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    Ggamma-10 was amplified by PCR, which added a Bam…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55192-plasmid-map-sequence-id-115049

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  9. YFP(159-238)-gamma-12 in pcDNAI/Amp

    Plasmid
    #55194
    Purpose
    A carboxyl-terminal YFP fragment was fused to Ggamma-12. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Publication
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    YFP(159-238)-gamma-12 (GNG12 Aequorea victoria, Human)
    Tags
    YFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    Ggamma-12 was amplified by PCR, which added a Bam…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55194-plasmid-map-sequence-id-115063

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  12. pCDNA_B2AR-q-pep

    Plasmid
    #47437
    Purpose
    FRET sensor for beta2-adrenergic receptor
    Depositor
    Sivaraj Sivaramakrishnan
    Publication
    Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    beta2-adrenergic receptor (ADRB2 Human)
    Tags
    10 nm ER/K helix, HA, mCerulean, mCitrine, and q-…
    Expression
    Mammalian
    Promoter
    pCMV
    Available since
    Sept. 11, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. VIPR1-Tango

    Plasmid
    #66520
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VIPR1 (VIPR1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. CCR6-Tango

    Plasmid
    #66243
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CCR6 (CCR6 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. UKNP4.2.2

    Plasmid
    #98268
    Purpose
    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)
    Depositor
    Jonathan Ball
    Publication
    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HCV E1E2
    Expression
    Mammalian
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98268-plasmid-map-sequence-id-190091

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  18. pCMV-Sport6-CD9-pHuji

    Plasmid
    #130906
    Purpose
    Expression of CD9-pHuji for visualization of multivesicular body-plasma membrane fusion.
    Depositor
    DM Pegtel
    Publication
    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD9-pHuji (CD9 Synthetic, Human)
    Tags
    pHuji
    Expression
    Mammalian
    Mutation
    pHuji inserted between Asn50 and Asn51 in extrace…
    Promoter
    CMV
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    130906-plasmid-map-sequence-id-272543

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  20. UKNP5.2.1

    Plasmid
    #98385
    Purpose
    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)
    Depositor
    Jonathan Ball
    Publication
    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HCV E1E2
    Expression
    Mammalian
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    98385-plasmid-map-sequence-id-190105

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Showing: 2081 - 2100 of 4673 results