We narrowed to 16,534 results for: por

1. pEGFP-C1X-Nup93

   Plasmid

   #87336

   Purpose

   To express human Nup93 in mammalian cells

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   NUP93 (NUP93 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   shRNA resistant changes

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMC-6-Ruby-9

   Plasmid

   #197740

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   Ruby reporter tricistronic CDS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. YFP-HA-DAT

   Plasmid

   #90244

   Purpose

   Expresses YFP-HA-DAT in mammalian cells

   Depositor

   Alexander Sorkin

   Article

   Sorkina et al J Neurosci. 2006 Aug 2;26(31):8195-205.

   Insert

   DAT (dopamine transporter) (SLC6A3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   residues 193–203 of human DAT were replaced in YF…

   Promoter

   CMV

   Available since

   June 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. hSyn1-sypHy-RGECO

   Plasmid

   #84078

   Purpose

   Combined fluorescent reporter for both presynaptic calcium influx and vesicle excoytosis

   Depositor

   Juan Burrone

   Article

   Jackson et al Front Synaptic Neurosci. 2016 Jul 26;8:2

   Insert

   sypHy-RGECO
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Donor_eGP2AP_RC

   Plasmid

   #133784

   Purpose

   Library reporter vector/integration vector. Contains library cloning sites MluI and SpeI upstream of a terminator and downstream of a BxB1 attB site. Library integration occurs through BxB1 site.

   Depositor

   Sriram Kosuri

   Article

   Davis et al Cell Syst. 2020 Jul 22;11(1):75-85.e7. d

   Type

   Empty backbone

   Use

   Tags

   eGFP-P2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMuLE_ENTR_TOP-iRFP_L5-L4

   Plasmid

   #113866

   Purpose

   Single pathway reporter; wnt promoter-driven expression of iRFP; MultiSite Gateway entry vector.

   Depositor

   Manfred Ogris

   Article

   Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

   Inserts

   TOP (wnt dependent promoter)
   iRFP713
   

   Use

   Mule gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET30b_OmpAT7Sav

   Plasmid

   #138589

   Purpose

   T7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 ori

   Depositor

   Markus Jeschek

   Article

   Jeschek et al Nature. 2016 Sep 29;537(7622):661-665. d

   Insert

   T7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export
   

   Use

   Synthetic Biology

   Tags

   OmpA export signal and T7 tag

   Expression

   Bacterial

   Mutation

   Promoter

   PT7

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR iRFP-ZtSH2

   Plasmid

   #188627

   Purpose

   Fluorescent reporter for CD3ε/ZtSH2 pYtag biosensor

   Depositor

   Jared Toettcher

   Article

   Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

   Insert

   ZtSH2
   

   Use

   Lentiviral

   Tags

   iRFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1X-Nup88

   Plasmid

   #87338

   Purpose

   To express human Nup88 in mammalian cells

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   NUP88 (NUP88 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLHDR03_mCherry_tCD19_unique guide

    Plasmid

    #124062

    Purpose

    Construction of 1st donor for scarless genome editing with unique guide

    Depositor

    Matthew Porteus

    Article

    Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

    Inserts

    mCherry
    tCD19
    

    Use

    Mouse Targeting; Human targeting

    Tags

    Expression

    Mutation

    Promoter

    Human UbC modified

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNMT

    Plasmid

    #42578

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    N-myristoyl tranferase (NMT1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    two silent mutations T690C and T942C

    Promoter

    T7

    Available since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pGilesSHdI_redgreen_2395A_His6_triple red_pLeft1806_His6_double green

    Plasmid

    #170048

    Purpose

    Tri-Responsive Reporter Gene Construct for redgreen, triple red, and double green

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mScarlet
    COmScarlet
    mRuby3
    mWasabi dN
    mWasabi CO
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. AMPK-SPARK

    Plasmid

    #226255

    Purpose

    phases-of-separation based activity reporter of AMPK

    Depositor

    Roland Malli

    Article

    Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

    Insert

    AMPK-SPARK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCEP4-SERT-myc

    Plasmid

    #107456

    Purpose

    Mammalian expression plasmid for codon optimized hSERT with internal MYC tag inserted in ECL2

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. single cut NH-donor

    Plasmid

    #83575

    Purpose

    eGFP reporter donor containing single SA cutting site for NHEJ knock-in

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    eGFP
    

    Use

    Nhej donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. 8xGliBS-IVS2-mCherry-NLS-Odc1-polyA-Tol2

    Plasmid

    #84603

    Purpose

    Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

    Depositor

    James Chen

    Article

    Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

    Insert

    mCherry
    

    Use

    Zebrafish transgenesis

    Tags

    ODC1 destabilizing element

    Expression

    Mutation

    Promoter

    8xGliBS-delta-crystallin minimal promoter

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. double cut NH-donor

    Plasmid

    #83576

    Purpose

    eGFP reporter donor containing double SA cutting sites for NHEJ knock-in

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    eGFP
    

    Use

    Nhej donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. psiCHECK2-2A-3xFLAG-SBP-2A

    Plasmid

    #159584

    Purpose

    Luciferase reporter for ribosome pausing control

    Depositor

    Shintaro Iwasaki

    Article

    Han et al Cell Rep. 2020 May 5;31(5):107610. doi:

    Inserts

    2A-3xFLAG-SBP-2A
    EIF2S3 5UTR
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP(C3)-Nup153-N

    Plasmid

    #64318

    Purpose

    GFP-tagged mammalian expression of N-terminal domain of Nup153

    Depositor

    Birthe Fahrenkrog

    Article

    Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/

    Insert

    Nup153 (NUP153 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N-terminal domain

    Promoter

    CMV

    Available since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pCFD2-dU6:2gRNA

    Plasmid

    #49409

    Purpose

    expression of gRNA under control of the Drosophila U6:2 promoter

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    dU6-2:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6-2

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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