We narrowed to 10,632 results for: t7

1. CPH3949_HIS-MBP-TEV-Tom1p-HECT-2850-3268

   Plasmid

   #227089

   Purpose

   Bacterial expression of HIS-MBP-TEV tagged Tom1p HECT domain (2850-3268)

   Depositor

   Chris Hill

   Insert

   tom1p-HECT (TOM1 Budding Yeast)
   

   Tags

   HIS-MBP-TEV_cleavage_site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CPH3747_GST-TEV-Spt6p-tSH2-1223-1452

   Plasmid

   #227086

   Purpose

   Bacterial expression of R1282H single-mutant GST-tagged Spt6 tSH2 domain (1223-1452)

   Depositor

   Chris Hill

   Insert

   spt6-tsh2 (SPT6 Budding Yeast)
   

   Tags

   GST-TEV_cleavage_site

   Expression

   Bacterial

   Mutation

   R1282H

   Promoter

   T7

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pet28a+_Yjjg

   Plasmid

   #220228

   Purpose

   Bacterial expression of Yjjgin E. coli BL21_DE3 (pLys) or similar

   Depositor

   Bernd Nidetzky

   Insert

   Pyrimidine 5'-nucleotidase YjjG (yjjG E.coli)
   

   Tags

   Histag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pet15b _yein

   Plasmid

   #220226

   Purpose

   Bacterial expression of YeiN in E. coli BL21_DE3 (pLys) or similar

   Depositor

   Bernd Nidetzky

   Insert

   psug (psuG E.coli)
   

   Tags

   Histag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. 8VL7

   Plasmid

   #220883

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   TREX1 (TREX1 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 20, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pET28-Flag-RBM24

   Plasmid

   #208621

   Purpose

   for E. coli expression of Flag tagged human RBM24 (ENST00000379052)

   Depositor

   Quanfu Ma

   Insert

   human RBM24 (ENST00000379052) coding region (RBM24 Human)
   

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7CFE1_EVA71_VP12A

   Plasmid

   #212977

   Purpose

   Encodes EV-A71 4643 VP12A optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   Enterovirus A71 4643 VP12A
   

   Use

   Mammalian cell free protein expression

   Tags

   HA tag

   Promoter

   T7

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE80l B-CC-B

   Plasmid

   #196479

   Purpose

   Protein expression of SpyCatcher-ELP-CarHCC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCC

   Depositor

   Fei Sun

   Insert

   B-CC-B
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Talin1-FERM

   Plasmid

   #110563

   Purpose

   bacterial expression of Talin 1

   Depositor

   Tina Izard

   Insert

   Talin 1 (Tln1 Mouse)
   

   Tags

   (His)8

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. TBXTA-c022

    Plasmid

    #139759

    Purpose

    Protein expression/crystallography

    Depositor

    Opher Gileadi

    Insert

    TBXT, DNA-binding domain, G177D (TBXT Human)
    

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    G177D; contains only amino acids E41-A214

    Promoter

    T7

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmSod1-Q42L;Q123A-N’His

    Plasmid

    #117710

    Purpose

    mutation on stability/solubility of SOD1

    Depositor

    Elizabeth Fisher

    Insert

    Sod1 (Sod1 Mouse)
    

    Tags

    His tag with Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Q42L; Q123A

    Promoter

    T7

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACYCduet-mxLOX2-EH

    Plasmid

    #104979

    Purpose

    Biosynthesis of oxylipins by microbial enzymes

    Depositor

    Deokkun Oh

    Inserts

    MXAN_1744 (MXAN_1744 Myxococcus xanthus)
    MXAN_1644 (MXAN_1644 Myxococcus xanthus)
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. 5K29 (PP-BRD3)

    Plasmid

    #101883

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PP-BRD3
    

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. 5TCM (PP-BRD3)

    Plasmid

    #101888

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PP-BRD3
    

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. Mmadhc

    Plasmid

    #74662

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    Mmadhc (MMADHC Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pJK611

    Plasmid

    #72532

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit (AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Expression

    Bacterial

    Promoter

    T7 and n/a

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJK692

    Plasmid

    #71643

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His17>Trp/Ser38>Thr mutant (TdPurE2-H17W,S38T)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Expression

    Bacterial

    Mutation

    changes histidine-17 to tryptophan, serine-38 to …

    Promoter

    T7

    Available Since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJK653

    Plasmid

    #71630

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Thr/Phe79>Trp mutant (TdPurE2-S38T,F79W)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Expression

    Bacterial

    Mutation

    changes serine-38 to threonine, phenylalanine-79 …

    Promoter

    T7

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK654

    Plasmid

    #71631

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Ala/Phe79>Trp mutant (TdPurE2-S38A,F79W)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Expression

    Bacterial

    Mutation

    changes serine-38 to alanine, phenylalanine-79 to…

    Promoter

    T7

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK655

    Plasmid

    #71632

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asp/Phe79>Trp mutant (TdPurE2-S38D,F79W)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Expression

    Bacterial

    Mutation

    changes serine-38 to aspartate, phenylalanine-79 …

    Promoter

    T7

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge