We narrowed to 2,368 results for: pcas9

1. SOX17-3'gRNA

   Plasmid

   #210465

   Purpose

   gRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-in

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   SOX17 (SOX17 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMonAID_nCas9-PmCDA_Hyg_ALS

   Plasmid

   #91693

   Purpose

   Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A for nickase Cas9

   Promoter

   2x35S

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

   Plasmid

   #113039

   Purpose

   AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDicAID_nCas9-PmCDA-2A-NptII_ETR

   Plasmid

   #91695

   Purpose

   Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A for nickase Cas9

   Promoter

   PcUbi

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc-y1sgRNA-Cas9

   Plasmid

   #49331

   Purpose

   Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

   Depositor

   Ji-Long Liu

   Article

   Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

   Inserts

   Cas9
   dU6-sgRNA
   y1 sgRNA (y Fly)
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Insect

   Mutation

   Human codon optimised

   Promoter

   Actin-5c and Drosophila U6

   Available Since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. Polymerase expression - pCMV-Phi29(+exo) (LM2759)

   Plasmid

   #208964

   Purpose

   Unfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29(+exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330 LC-FKBP-Cas9

   Plasmid

   #162724

   Purpose

   Mammalian expression

   Depositor

   Amit Choudhary

   Article

   Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

   Insert

   FKBP F36V
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA

   Plasmid

   #176458

   Purpose

   Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoter

   Depositor

   Hui Yang

   Article

   Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

   Insert

   humanized S. pyogenes Cas9-XTEN-Ec73RT fusion
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMonAID_dCas9-PmCDA_Hyg_ALS

   Plasmid

   #91692

   Purpose

   Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A and H840A for dead Cas9

   Promoter

   2x35S

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-ACBEmax

    Plasmid

    #139106

    Purpose

    Mammalian ACBEmax expression vector (pSI-632)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpyCas9 PE2*-3HA

    Plasmid

    #169854

    Purpose

    Mammalian Expression, SpyCas9 PE2* prime editor with 3HA-tag

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SpCas9-H840A-star-M-MLV-3HA
    

    Use

    Tags

    3XHA tag, bpSV40 NLS and SV40 NLS, and cmyc-NLS a…

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Polymerase expression - pCMV-Phi29 (LM2708)

    Plasmid

    #208965

    Purpose

    Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Phi29(-exo;D169A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330 NL-FKBP-Cas9

    Plasmid

    #162723

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUDP012

    Plasmid

    #101167

    Purpose

    E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Insert

    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. VRQR-HF1 variant

    Plasmid

    #138563

    Purpose

    Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRQR-HF1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. FUG-T2A-Cas9

    Plasmid

    #75346

    Purpose

    Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP (via t2a)

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330 N-FKBP-Cas9

    Plasmid

    #162727

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. L-CRISPR-CTN (MLL-ctrl)

    Plasmid

    #69215

    Purpose

    Advanced lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; control, MLL-sgRNA + luciferase-sgRNA

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV promoter
    P2A-mNeonGreen
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRI018-pKW20088-PelcA-mCherry-TtrpC-PU3-E1-Cas9Scaffold-8xT

    Plasmid

    #140205

    Purpose

    Proof-of-concept of CRISPR/dSpCas9-VPR activation, encoding PelcA-mCherry along with the sgRNA E1 expression cassette, targeting PelcA.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Inserts

    mCherry
    sgRNA E1
    

    Use

    CRISPR and Synthetic Biology; Proof-of-concept te…

    Tags

    Expression

    Mutation

    G174D

    Promoter

    PelcA and U3

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGMC00034 (aka pMC0235)

    Plasmid

    #210751

    Purpose

    Non-targeting guide RNA

    Depositor

    Raj Chari

    Article

    Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

    Insert

    Non-targeting guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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