165,971 results

1. pSEVA63-GG

   Plasmid

   #208015

   Purpose

   for cloning homology arms, with GFP as counter selection marker

   Depositor

   Pablo Ivan Nikel

   Article

   Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

   Insert

   msfGFP
   

   Promoter

   14G

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBMN mCherry-OPTN(D474N)

   Plasmid

   #119683

   Purpose

   For mammalian expression of UBD mutant OPTN

   Depositor

   Michael Lazarou

   Article

   Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

   Insert

   OPTN (OPTN Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   D474N

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgOpti

   Plasmid

   #85681

   Purpose

   Lentiviral vector to express sgRNAs. The cloning site is BsmBI.

   Depositor

   Eric Lander, David Sabatini

   Article

   Fulco et al Science. 2016 Sep 29. pii: aag2445.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)

   Plasmid

   #225755

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-UbC-VP64-dCas9-VP64

   Plasmid

   #232179

   Purpose

   Lentiviral cassette that expresses 2xVP64-dCas9 fusion in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

   Insert

   VP64-dCas9-VP64
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   hUbC

   Available Since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. AbVec2.0-mIgkc

   Plasmid

   #127157

   Purpose

   Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), kappa constant region

   Depositor

   Hedda Wardemann

   Article

   Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

   Insert

   Igkc (Igkc Mouse)
   

   Expression

   Mammalian

   Promoter

   hCMV IE1 gene promoter

   Available Since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3puro-mGFP-ROCK2

   Plasmid

   #225472

   Purpose

   Express mEGFP-fusion protein of ROCK2

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   ROCK2 (ROCK2 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1/Puro-CAG-ASAP2s

   Plasmid

   #101274

   Purpose

   Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

   Depositor

   Francois St-Pierre

   Article

   Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

   Insert

   ASAP2s
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSETA_crGE

   Plasmid

   #172927

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.

   Depositor

   Arnold Boersma

   Article

   Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

   Insert

   CrowdingSensorGE
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXS-IRES-BLAST flag-CAD

    Plasmid

    #169818

    Purpose

    Expresses C-terminal flag-tagged CAD in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCCC -> AGTC silent mutations at nt527-530 eli…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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