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pOC4 Grin1-smFP-V5 KI

Plasmid

#183439

Purpose

FlpON knock-in for GluN1-smFP-V5 (amino acid position: A20)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP V5 donor

Use

CRISPR

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 Gria1-GFP KI

Plasmid

#183430

Purpose

CreOFF knock-in for GluA1-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pRB131

Plasmid

#180174

Purpose

pAAV construct GluN1A714L (shRNA resistant)

Depositor

Richard Bergeron

Article

Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

Insert

GluN1 (Grin1 Rat)

Use

AAV

Tags

Phluorin

Expression

Mammalian

Mutation

A714L

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLIC-His-GBAi W211A

Plasmid

#171756

Purpose

For the expression of His-GBAi W211A in bacteria

Depositor

Mikel Garcia-Marcos

Article

Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

Insert

GBAi

Tags

His

Expression

Bacterial

Available Since

Nov. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-4T Doc2beta C2AB(125) H158C

Plasmid

#134691

Purpose

Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Double C2 protein beta (Doc2b Rat)

Tags

GST

Expression

Bacterial

Mutation

H158C, C145A, C217A, C249A, C290A, C338A, C387A

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Doc2beta C2AB(125) F222C

Plasmid

#134692

Purpose

Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Double C2 protein beta (Doc2b Rat)

Tags

GST

Expression

Bacterial

Mutation

F222C, C145A, C217A, C249A, C290A, C338A, C387A

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Doc2beta C2AB(125) A298C

Plasmid

#134693

Purpose

Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Double C2 protein beta (Doc2b Rat)

Tags

GST

Expression

Bacterial

Mutation

A298C, C145A, C217A, C249A, C290A, C338A, C387A

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Syt1 C2AB(140) M173C

Plasmid

#134696

Purpose

Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Synaptotagmin 1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

M173C, C277A

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Syt1 C2AB(140) F234C

Plasmid

#134697

Purpose

Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Synaptotagmin 1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

F234C, C277A

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Syt1 C2AB(140) V304C

Plasmid

#134698

Purpose

Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Synaptotagmin 1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

V304C, C277A

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Syt1 C2AB(140) I367C

Plasmid

#134699

Purpose

Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Synaptotagmin 1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

I367C, C277A

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pIVT-EGFP-ratCENP-C

Plasmid

#174445

Purpose

in vitro transcription template for rat CENP-C (N-ter EGFP tagged)

Depositor

Michael Lampson

Article

Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

Insert

Centromere protein C

Use

In vitro transcription template

Tags

EGFP

Promoter

T7 SP6

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC1535 pscAAV mU6 shRNA(scram) CMV-IE Nuc-EYFP

Plasmid

#135563

Purpose

An AAV vector expressing scrambled shRNA and a nuclear EYFP reporter

Depositor

Christopher Richie

Article

Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

Inserts

shRNA (scrambled)
Nuc-EYFP

Use

AAV and RNAi

Tags

3xNLS

Expression

Mammalian

Promoter

CMV-IE and mU6

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLIC-His-GBAi

Plasmid

#171755

Purpose

For the expression of His-GBAi in bacteria

Depositor

Mikel Garcia-Marcos

Article

Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

Insert

GBAi

Tags

His

Expression

Bacterial

Available Since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CMV-mEGFP(A206K)-paCaMKII(I539E)-ShadowG

Plasmid

#167478

Purpose

Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(I539E)-ShadowG

Tags

ShadowG and mEGFP(A206K)

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP(A206K)-paCaMKII(C450A)-ShadowG

Plasmid

#167479

Purpose

Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(C450A)-ShadowG

Tags

ShadowG and mEGFP(A206K)

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pETM10 with rat Δ192Annexin A11

Plasmid

#164498

Purpose

For bacterial expression of rat His-tagged Δ192Annexin A11

Depositor

Anni Vedeler

Article

Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

Insert

rat Δ192Annexin A11 (Anxa11 Rat)

Tags

His

Expression

Bacterial

Mutation

N-terminal truncation

Promoter

T7

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pETM10 with rat Δ188Annexin A11

Plasmid

#164497

Purpose

For bacterial expression of rat His-tagged Δ188Annexin A11

Depositor

Anni Vedeler

Article

Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

Insert

rat Δ188Annexin A11 (Anxa11 Rat)

Tags

His

Expression

Bacterial

Mutation

N-terminal truncation

Promoter

T7

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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Ad-HisFlag-USP19ER

Plasmid

#155244

Purpose

for generating adenoviruses expressing the ER isoform of rat USP19

Depositor

Simon Wing

Article

Wiles et al Mol Biol Cell. 2015 Mar 1;26(5):913-23.

Insert

USP19 (ER isoform) (Usp19 Rat)

Use

Adenoviral

Tags

6XHis-3XFlag

Mutation

H118R- please see dep. comments

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCIS-rPAM3

Plasmid

#145803

Purpose

expresses rat PAM3 in mammalian cells

Depositor

Betty Eipper, Richard Mains

Article

Milgram et al J Cell Biol. 1994 Jan;124(1-2):33-41. do

Insert

rat PAM3

Expression

Mammalian

Mutation

See depositor comments below

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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