2,384 results

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pLZRS-TrkC-mCherry

Plasmid

#182949

Purpose

To express the rat TrkC fused in the C-terminus with mCherry

Depositor

Pantelis Tsoulfas

Insert

TRk-C-mCherry (Ntrk3 Rat, Synthetic)

Use

Retroviral and Synthetic Biology

Tags

mCherry

Expression

Mammalian

Promoter

MMLV

Available since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK54 pHomL-RPL18Bp-Neon(gfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)

Plasmid

#179038

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-2xPH(PLCd), a marker for the plasma membrane

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-2xPH(PLCd)

Expression

Yeast

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE Tubb3-2xGFP KI

Plasmid

#183443

Purpose

GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and 2xGFP donor

Use

CRISPR

Expression

Mammalian

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC4 Kcnn2-smFP-myc KI

Plasmid

#183438

Purpose

FlpON knock-in for SK2-smFP-myc (amino acid position: T571)

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP myc donor

Use

CRISPR

Expression

Mammalian

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 GFP-Mpp2 KI

Plasmid

#183434

Purpose

CreOFF knock-in for GFP-MPP2 (amino acid position: A4)

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Expression

Mammalian

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC5 smFP-HA-Cacna1e KI

Plasmid

#183441

Purpose

FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP HA donor

Use

CRISPR

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 Gria1-Halo KI

Plasmid

#183433

Purpose

CreOFF knock-in for GluA1-Halo (amino acid position: stop codon)

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and Halo donor

Use

CRISPR

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE Tubb3-3xGFP KI

Plasmid

#183444

Purpose

GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and 3xGFP donor

Use

CRISPR

Expression

Mammalian

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 Tubb3 no donor

Plasmid

#183431

Purpose

CreOFF gRNA expression for beta3-tubulin (amino acid position: stop codon)

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA

Use

CRISPR

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 GFP-Actb KI

Plasmid

#183429

Purpose

CreOFF knock-in for GFP-beta-actin (amino acid position: before start codon)

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOC4 Grin1-smFP-V5 KI

Plasmid

#183439

Purpose

FlpON knock-in for GluN1-smFP-V5 (amino acid position: A20)

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP V5 donor

Use

CRISPR

Expression

Mammalian

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 Gria1-GFP KI

Plasmid

#183430

Purpose

CreOFF knock-in for GluA1-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pRB131

Plasmid

#180174

Purpose

pAAV construct GluN1A714L (shRNA resistant)

Depositor

Richard Bergeron

Publication

Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

Insert

GluN1 (Grin1 Rat)

Use

AAV

Tags

Phluorin

Expression

Mammalian

Mutation

A714L

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLIC-His-GBAi W211A

Plasmid

#171756

Purpose

For the expression of His-GBAi W211A in bacteria

Depositor

Mikel Garcia-Marcos

Publication

Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

Insert

GBAi

Tags

His

Expression

Bacterial

Available since

Nov. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-4T Doc2beta C2AB(125) H158C

Plasmid

#134691

Purpose

Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Publication

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Double C2 protein beta (Doc2b Rat)

Tags

GST

Expression

Bacterial

Mutation

H158C, C145A, C217A, C249A, C290A, C338A, C387A

Available since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Doc2beta C2AB(125) F222C

Plasmid

#134692

Purpose

Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Publication

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Double C2 protein beta (Doc2b Rat)

Tags

GST

Expression

Bacterial

Mutation

F222C, C145A, C217A, C249A, C290A, C338A, C387A

Available since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Doc2beta C2AB(125) A298C

Plasmid

#134693

Purpose

Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Publication

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Double C2 protein beta (Doc2b Rat)

Tags

GST

Expression

Bacterial

Mutation

A298C, C145A, C217A, C249A, C290A, C338A, C387A

Available since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Syt1 C2AB(140) M173C

Plasmid

#134696

Purpose

Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Publication

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Synaptotagmin 1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

M173C, C277A

Available since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Syt1 C2AB(140) F234C

Plasmid

#134697

Purpose

Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Publication

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Synaptotagmin 1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

F234C, C277A

Available since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Syt1 C2AB(140) V304C

Plasmid

#134698

Purpose

Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Publication

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Synaptotagmin 1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

V304C, C277A

Available since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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