Addgene

We narrowed to 8,185 results for: FIE

Showing: 2121 - 2140 of 8185 results

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pC-(loxP2-attB2-SA(0)-T2A-Gal4-Hsp70)

Plasmid

#62954

Purpose

Singlet donor (phase 0) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pC-(loxP2-attB2-SA(1)-T2A-Gal4-Hsp70)

Plasmid

#62955

Purpose

Singlet donor (phase 1) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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p306-BrdU-Inc

Plasmid

#71792

Purpose

BrdU-incorporating plasmid with selectable marker URA3

Depositor

Oscar Aparicio

Article

Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

Inserts

Thymidine Kinase
ENT1 (SLC29A1 Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1 and GPD

Available since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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p405-BrdU-Inc

Plasmid

#71791

Purpose

BrdU-incorporating plasmid with selectable marker LEU2

Depositor

Oscar Aparicio

Article

Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

Inserts

Thymidine Kinase
ENT1 (SLC29A1 Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1 and GPD

Available since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-TRE3G-LbNOX-Flag

Plasmid

#234980

Purpose

Expresses LbNOX in mouse cell lines

Depositor

Denis Titov

Article

Choe et al Cell Chem Biol. 2025 Apr 17;32(4):620-63

Insert

LbNOX

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-TRE3G-mitoLbNOX-Flag

Plasmid

#234981

Purpose

Expresses mitochondrial LbNOX in mouse cell lines

Depositor

Denis Titov

Article

Choe et al Cell Chem Biol. 2025 Apr 17;32(4):620-63

Insert

mito-LbNOX

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV MP-84 mRFP

Plasmid

#206926

Purpose

The micro promoter MP-84 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (84 bp) allows delivery of large genes in AAV.

Depositor

Markus Grompe

Article

Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2

Insert

mRFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

MP-84

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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cfSGFP2-anti-AlfaTag nanobody

Plasmid

#171818

Purpose

Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

Depositor

Ikuo Wada

Article

Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

Insert

cfSGFP2-anti-AlfaTag nanobody

Use

Tags

6xHis and SEP tag (6xD)

Expression

Bacterial

Mutation

Promoter

Available since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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lenti-25xUPRE-minP-EGFP

Plasmid

#159668

Purpose

EGFP reporter plasmid containing 25 tandem repeats of the the unfolded protein response element (UPRE)

Depositor

Seiichi Oyadomari

Article

Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

Insert

25xUPRE-minP-EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET29b-AK1-His

Plasmid

#124135

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Takuya Ueda, Sebastian Maerkl

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Adenylate kinase (AK1 Chicken)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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tdTomato-anti-AlfaTag nanobody

Plasmid

#171819

Purpose

Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

Depositor

Ikuo Wada

Article

Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

Insert

tdTomato-anti-AlfaTag nanobody

Use

Tags

6xHis and SEP tag (6xD)

Expression

Bacterial

Mutation

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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Cerulean-2xFv-hRIPK3

Plasmid

#106079

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

Cerulean-2xFv-hRIPK3 (RIPK3 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only
pBAD02_CBASS_Escherichia_coli_SLK172

Plasmid

#224395

Purpose

For expression of the type I CBASS system encoded by Escherichia coli SLK172

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

EcCdnD_4TM

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MAP4K7

Plasmid

#229402

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MAP4K7 (TNIK Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBITE-EGFRvIII-F269-OKT3

Plasmid

#190681

Purpose

Encodes an EGFRvIII-CD3 BITE protein (F269 clone)

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Anti-EGFRvIII-scFv-F269-modularized-OKT3-HisTag

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pv6_CBX1_2xChromo

Plasmid

#179393

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX1_2xChromo

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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lenti-25xERSE2-minP-EGFP

Plasmid

#159667

Purpose

EGFP reporter plasmid containing 25 tandem repeats of the ER stress response element-2 (ERSE2)

Depositor

Seiichi Oyadomari

Article

Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

Insert

25xERSE2-minP-EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 5, 2021

Availability

Academic Institutions and Nonprofits only

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pPEPY-PF6-lacI

Plasmid

#85589

Purpose

Work as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae

Depositor

Jan-Willem Veening

Article

Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

Insert

lacI

Use

Tags

Expression

Bacterial

Mutation

Promoter

PF6

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pJWV102-PL-dCas9

Plasmid

#85588

Purpose

Integrate plasmid of Streptococcus pneumoniae, for chromosome integration of IPTG-inducible dCas9sp

Depositor

Jan-Willem Veening

Article

Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

Insert

dCas9sp

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PLsp

Available since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pSprayerHE

Plasmid

#165150

Purpose

Sprayer plasmid for PROBER encoding BASU-GAL4 fusion (High Expression)

Depositor

Paul Khavari

Article

Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

Insert

BASU-GAL4

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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