We narrowed to 4,110 results for: LYC;

1. pGL3-sgRab18.N-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #129418

   Purpose

   Encoding Cas9 and sgRAB18.N for CRISPR/Cas9 mediated HDR tagging of endogenous human RAB18 N-terminus

   Depositor

   Elina Ikonen

   Article

   Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

   Insert

   SpCas9 and sgRAB18.N (RAB18 Human)
   

   Use

   CRISPR

   Tags

   T2A-mCherry-P2A-Puro

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.3 N-MYC TIP60

   Plasmid

   #213003

   Purpose

   expresses human TIP60

   Depositor

   Sheng-cai Lin

   Article

   Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

   Insert

   KAT5 (KAT5 Human)
   

   Tags

   MYC

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. SF-SON IRES Tomato

   Plasmid

   #214403

   Purpose

   Expresses human SOX10, OLIG2, NKX6.2 and tdTomato

   Depositor

   Tanja Kuhlmann

   Article

   Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5

   Plasmid

   #111818

   Purpose

   Quadcistronic construct: Co-express four genes by three 2A peptides -- T2A, P2A and E2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   Mef2c (Mef2c Mouse)
   Tdtomato
   Gata4 (Gata4 Mouse)
   Tbx5
   

   Use

   Cloning intermediate

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE

   Plasmid

   #51509

   Purpose

   Can be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Has Service

   AAV1

   Insert

   tdTomato-T2A-SypEGFP
   

   Use

   AAV

   Promoter

   phSyn1

   Available Since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUW-SOK

   Plasmid

   #20327

   Depositor

   Rudolf Jaenisch

   Article

   Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

   Insert

   Sox2-F2A-Oct4-T2A-Klf4 (Sox2, Pou5f1, Klf4 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBOBI TP53 S46D

   Plasmid

   #213002

   Purpose

   expresses human P53

   Depositor

   Sheng-cai Lin

   Article

   Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

   Insert

   P53 (TP53 Human)
   

   Expression

   Mammalian

   Mutation

   S46D

   Promoter

   cmv

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQCXIZ GFP PODXL delta PBM + Ezrin

   Plasmid

   #202418

   Purpose

   Expression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; doesn't bind NHERF1/2) fused to EZRIN

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   PODXL (PODXL Human)
   

   Use

   Lentiviral

   Tags

   Ezrin and GFP

   Mutation

   delete PDZ binding motif

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSF-DUET1-DHDDS/Sumo-NgBR

   Plasmid

   #203162

   Purpose

   Expression of human cis-prenyltransferase complex for purification

   Depositor

   William Sessa

   Article

   Edani et al Proc Natl Acad Sci U S A. 2020 Aug 25;11

   Inserts

   DHDDS (DHDDS Human)
   NUS1 dehydrodolichyl diphosphate synthase subunit (NUS1 Human)
   

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   truncation

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti PGK mKate2-OGT D554N (Puro)

    Plasmid

    #154292

    Purpose

    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, D554N variant, no Ser/Thr glycosylation)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    2x FLAG and mKate2

    Expression

    Mammalian

    Mutation

    D554N mutation (Ser/Thr glycosylation-incompetent)

    Promoter

    PGK

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBOBI N-HA AXIN1

    Plasmid

    #213000

    Purpose

    expresses mouse AXIN1

    Depositor

    Sheng-cai Lin

    Article

    Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

    Insert

    AXIN1 (Axin1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223-TGFBR3

    Plasmid

    #23478

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    TGFBR3 (TGFBR3 Human)
    

    Use

    Gateway donor vector

    Mutation

    C44T, 1MM

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pBOBI C-FLAG PHGDH V261M

    Plasmid

    #212999

    Purpose

    expresses human PHGDH

    Depositor

    Sheng-cai Lin

    Article

    Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

    Insert

    PHGDH (PHGDH Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    V261M

    Promoter

    cmv

    Available Since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL3-sgACSL3.C-Cas9-P2A-Puro

    Plasmid

    #129412

    Purpose

    Encoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminus

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    SpCas9 and sgACSL3.C (ACSL3 Human)
    

    Use

    CRISPR

    Tags

    P2A-Puro

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQCXIZ GFP PODXL delta PBM + FBM

    Plasmid

    #202416

    Purpose

    Expression of GFP-tagged PODXL PDZ-binding and FERM-binding motif mutation

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Mutation

    Lacking DTHL, and H477/R479/S481>AAA mutation

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3-Basic-ACSL3-sfGFP(C) HDR template

    Plasmid

    #129413

    Purpose

    HDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFP

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    ACSL3 HDR template (ACSL3 Human)
    

    Use

    CRISPR and TALEN; Endogenous tagging hdr template

    Tags

    sfGFP

    Expression

    Mammalian

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQCXIZ GFP PODXL FKBP 4K>R

    Plasmid

    #202421

    Purpose

    Expression of ubiquitination-deficient GFP-tagged PODXL with FKBP-rapamycin binding (FRB) dimerization domain

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    FKBP and GFP

    Mutation

    K431/525/526/547R

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. str-KDEL_Stx5LdeltaER-SBP-mCitrine

    Plasmid

    #154846

    Purpose

    Synchronize trafficking of Stx5LdeltaER (M55V) from the ER, for use with FLIM (RUSH system)

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    Stx5 (Long isoform) (STX5 Human)
    

    Tags

    Streptavidin Binding Protein (SBP) and mCitrine

    Expression

    Mammalian

    Mutation

    Changed Methionine 55 to Valine, causes loss of S…

    Promoter

    CMV

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUDP123

    Plasmid

    #107269

    Purpose

    pUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
    

    Expression

    Yeast

    Promoter

    ScTDH3

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTM-RBDv2

    Plasmid

    #162785

    Purpose

    SARS-CoV-2 S protein receptor binding domain (RBD) expression in mammalian cells

    Depositor

    Ivan Vorobiev

    Article

    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi

    Insert

    SARS-CoV-2 S protein receptor binding domain (RBD) (S Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
    

    Tags

    10xHis, c-myc, and hTPA leader

    Expression

    Mammalian

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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