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YIp128-ADH-myc-E3(1)-CUbo-VSV

Plasmid

#212735

Purpose

Consitutive expression of myc-E3(1)-CUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-VSV and myc

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-ADH-CUbo-mCherry-VSV

Plasmid

#212734

Purpose

Consitutive expression of CUbo-mCherry-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

CUbo

Use

Integrative vector

Tags

mCherry-VSV

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-ADH-NUbo-mCherry-VSV

Plasmid

#212731

Purpose

Consitutive expression of NUbo-mCherry-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Use

Integrative vector

Tags

mCherry-VSV

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-H2B-His8

Plasmid

#212810

Purpose

Constitutive or doxycycline-inducible expression of NUbo-H2B-His8 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

H2B

Tags

8xHis and NUbo

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-His6-NUbo-H2B-His8

Plasmid

#212811

Purpose

Constitutive or doxycycline-inducible expression of His6-NUbo-H2B-His8 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

H2B

Tags

6xHis-NUbo and 8xHis

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-His8-H2B-CUbo

Plasmid

#212812

Purpose

Constitutive or doxycycline-inducible expression of His8-H2B-CUbo in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

H2B

Tags

8xHis and CUbo

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-His8-H2B-CUbo(K48R)

Plasmid

#212813

Purpose

Constitutive or doxycycline-inducible expression of His8-H2B-CUbo(K48R) in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

H2B

Tags

8xHis and CUbo(K48R)

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.U6gT28.3.hSyn.H2B.RFP

Plasmid

#170370

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B RFP

Use

AAV

Promoter

human Synapsin

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.U6gT28.13.hSyn.H2B.RFP

Plasmid

#170372

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B RFP

Use

AAV

Promoter

human Synapsin

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLV.U6gT28.4.EFS-NS.H2B-RFP

Plasmid

#170365

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B-RFP

Use

Lentiviral

Promoter

EFS-NS

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLV.U6gT28.3.EFS-NS.H2B-RFP

Plasmid

#170364

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B-RFP

Use

Lentiviral

Promoter

EFS-NS

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti6.3delta4 GPR3-V5

Plasmid

#172601

Purpose

Lentiviral plasmid expressing c-terminal V5-tagged GPR3

Depositor

Robert Screaton

Article

Iorio et al Commun Biol. 2021 Jul 23;4(1):907. doi:

Insert

G protein-Coupled Receptor 3 (GPR3 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSC51

Plasmid

#104825

Purpose

CRISPR/Cas9 2xplex gRNA targeting Glyma.12g075700, Glyma.11g145900 (Drb2ab). Also expresses Cas9 from rolD promoter from Gmubi promoter

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Insert

Glyma.12g075700, Glyma.11g145900

Use

CRISPR

Expression

Plant

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGMC00008

Plasmid

#166724

Purpose

sgRNA cloning backbone

Depositor

Raj Chari

Article

Choi et al Nat Commun. 2020 Jun 1;11(1):2718. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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Twitch-2C pRSETB

Plasmid

#104500

Purpose

fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2C

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only
mIFP-C-Src-7

Plasmid

#56213

Purpose

Localization: Membrane/Vesicles, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

C-Src (SRC Chicken)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mIFP12-Myopalladin-C-14

Plasmid

#56258

Purpose

Localization: Cytoskeleton , Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Myopalladin (MYPN Human)

Tags

mIFP12

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP12-Myotilin-C-14

Plasmid

#56260

Purpose

Localization: Cytoskeleton, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Myotilin (MYOT Human)

Tags

mIFP12

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pFastBac-GST-TEV-mCherry-TBK1

Plasmid

#198033

Purpose

Used for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Tags

GST-TEV and mCherry

Expression

Insect

Mutation

E696K

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pML104

Plasmid

#67638

Purpose

Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

Depositor

John Wyrick

Article

Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

Inserts

Cas9
single guide RNA expression cassette

Use

CRISPR

Expression

Yeast

Promoter

pSNR52 and pTDH3

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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