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We narrowed to 3,518 results for: mak

Showing: 2121 - 2140 of 3518 results

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pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry

Plasmid

#35510

Purpose

AAV expression of Ef1a-driven, cre-dependent, hChR2 variant for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123T and T159C

Promoter

Ef1a

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

Plasmid

#131003

Purpose

bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

Kv2.1-HA

Expression

Mutation

Promoter

CaMKIIa

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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KIF1A(1-393)-GCN4-2xmCherry-EF(C)

Plasmid

#61665

Purpose

Tandem mCherry-labeled, forced-dimeric, truncated kinesin-3 fused to EF Hand linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF1A(1-393)-GCN4-2xmCherry-EF(C) (KIF1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pHS0810 pcDNA3.1(+) CMV-SV40 NLS-OgeuIscB-nucleoplasmin NLS-3xHA

Plasmid

#176540

Purpose

Mammalian expression of nuclear localized OgeuIscB

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

OgeuIscB

Use

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-eArch 3.0-EYFP

Plasmid

#35516

Purpose

AAV expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

Arch3.0-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Enhanced trafficking signal and ER export signal …

Promoter

CaMKIIa

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pDEST-DHFR F[3]-N (LEU2)

Plasmid

#177798

Purpose

Gateway destination vector to insert genes of interest having a N-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRmine-eYFP-WPRE

Plasmid

#130988

Purpose

AAV vector to drive the expression of ChRmine-eYFP under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

eYFP

Expression

Mutation

Promoter

CaMKIIa

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-FLAG-SNAP

Plasmid

#175295

Purpose

Track mature FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with SNAP-tag

Use

Tags

SNAP-tag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-CaMKIIa-hChR2(C128S/D156A)-EYFP

Plasmid

#35501

Purpose

AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

C128S and D156A

Promoter

CaMKIIa

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pCXLE-hGLIS1

Plasmid

#37625

Purpose

Non-integrating (episomal) expression of human GLIS1

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

human GLIS1 (GLIS1 Human)

Use

Tags

Expression

Mammalian

Mutation

T110A, A187G, I221T

Promoter

CAG

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE

Plasmid

#130991

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

mScarlet-Kv2.1

Expression

Mutation

Promoter

CaMKIIa

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry

Plasmid

#35512

Purpose

AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123T and T159C

Promoter

CaMKIIa

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRmine-eYFP-WPRE

Plasmid

#130992

Purpose

AAV vector to drive the expression of ChRmine-eYFP under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

eYFP

Expression

Mutation

Promoter

hSyn

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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mIFP-H2B-C-10

Plasmid

#56224

Purpose

Localization: Nucleus/Histones, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

H2B (H2BC11 Human)

Use

Tags

mIFP

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP

Plasmid

#35511

Purpose

AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123T and T159C

Promoter

CaMKIIa

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-GBF1(E794K)

Plasmid

#206416

Purpose

Mammalian expression of hGBF1(E794K)

Depositor

Kazuhisa Nakayama

Article

Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

Insert

GBF1 (GBF1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E794K

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pMRXIP 3xFLAG-VMP1

Plasmid

#117416

Purpose

Expresses VMP1 tagged with 3xFLAG in mammalian cells

Depositor

Noboru Mizushima

Article

Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

Insert

VMP1 (VMP1 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pBMN mEGFP-NDP52

Plasmid

#188785

Purpose

For mammalian expression of NDP52 tagged with mEGFP

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NDP52 (CALCOCO2 Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-IZU-LAMP1-mRuby3

Plasmid

#222586

Purpose

Stable expression of LAMP1-mRuby3 in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

LAMP1 (LAMP1 Human)

Use

Retroviral

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-eArchT 3.0-EYFP

Plasmid

#35513

Purpose

Lentiviral expression of CaMKIIa-driven ArchT 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

eArchT 3.0-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Enhanced trafficking signal and ER export signal …

Promoter

CaMKIIa

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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