We narrowed to 1,112 results for: ENDO 2

1. pACYC-TtCas6-4xcrRNA4.5

   Plasmid

   #127764

   Purpose

   For expression of T. thermophilus Csm complex in E. coli (crRNA)

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

   Inserts

   Synthetic CRISPR array containing four identical T. thermophilus spacers
   Cas6A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for expression in E. coli

   Promoter

   T7

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. p.UTA.2.0 miR 19b-3p

   Plasmid

   #82442

   Purpose

   Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-19b-3p

   Depositor

   Jens Gruber

   Article

   Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

   Insert

   hsa-miR-19-3p
   

   Use

   Tags

   Expression

   Mutation

   Complementary region for hsa-miR-19-3p as sensor

   Promoter

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTJK511

   Plasmid

   #121468

   Purpose

   2µm HIS3 CNB1mutEF4

   Depositor

   Tami Kingsbury

   Article

   Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

   Insert

   CNB1mutEF4 (CNB1 Budding Yeast)
   

   Use

   Yeast high copy 2 micron vector

   Tags

   Expression

   Mutation

   Changed aspartic acid 144 to alanine

   Promoter

   Endogenous genomic promoter region

   Available since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTJK510

   Plasmid

   #121465

   Purpose

   2µm HIS3 CNB1mutEF3

   Depositor

   Tami Kingsbury

   Article

   Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

   Insert

   CNB1mutEF3 (CNB1 Budding Yeast)
   

   Use

   Yeast high copy 2 micron vector

   Tags

   Expression

   Mutation

   Changed aspartic acid 103 to alanine

   Promoter

   Endogenous genomic promoter region

   Available since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTJK509

   Plasmid

   #121464

   Purpose

   2µm HIS3 CNB1mutEF2

   Depositor

   Tami Kingsbury

   Article

   Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

   Insert

   CNB1mutEF2 (CNB1 Budding Yeast)
   

   Use

   Yeast high copy 2 micron vector

   Tags

   Expression

   Mutation

   Changed aspartic acid 66 to alanine

   Promoter

   Endogenous genomic promoter region

   Available since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTJK508

   Plasmid

   #121463

   Purpose

   2µm HIS3 CNB1mutEF1

   Depositor

   Tami Kingsbury

   Article

   Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

   Insert

   CNB1mutEF1 (CNB1 Budding Yeast)
   

   Use

   Yeast high copy 2 micron vector

   Tags

   Expression

   Mutation

   Changed aspartic acid 34 to alanine

   Promoter

   Endogenous genomic promoter region

   Available since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5-FLAG-p14

   Plasmid

   #42330

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

   Insert

   p14 (LAMTOR2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)

   Plasmid

   #156415

   Purpose

   kdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4A-G12D (KRAS Human)
   

   Use

   Zebrafish transgenesis

   Tags

   mTagBFP2

   Expression

   Mutation

   mutant KRAS4A-G12D

   Promoter

   kdrl

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-nSpRY(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES636)

   Plasmid

   #185491

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage nSpRY(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

   Depositor

   Benjamin Kleinstiver, Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage nSpRY(D10A)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-eGFP

   Expression

   Mammalian

   Mutation

   D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. JDW 859 (pTol2-kdrl:V5-mScarlet-I-hsKRAS4A-G12V-ac/mC)

    Plasmid

    #156416

    Purpose

    kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A-G12V with an alpha-crystallin mCerulean reporter flanked by Tol2 sites

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12V (KRAS Human)
    

    Use

    Zebrafish transgenesis

    Tags

    mScarlet-I

    Expression

    Mutation

    mutant KRAS4A-G12V

    Promoter

    kdrl

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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