We narrowed to 5,869 results for: nls

1. pY130 (pcDNA3.1-U6-huBsCpf1)

   Plasmid

   #92306

   Purpose

   Expression of humanized BsCpf1 and crRNA

   Depositor

   Feng Zhang

   Insert

   huBsCpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mEmerald-Nucleus-7

   Plasmid

   #54206

   Purpose

   Localization: Nucleus, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   SV40 T-Antigen Nuclear Localization Signal (NLS)
   

   Use

   Tags

   mEmerald-Nucleus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pY128 (pcDNA3.1-U6-huMb2Cpf1)

   Plasmid

   #92304

   Purpose

   Expression of humanized Mb2Cpf1 and crRNA

   Depositor

   Feng Zhang

   Insert

   huMb2Cpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pY129 (pcDNA3.1-U6-huMb3Cpf1)

   Plasmid

   #92305

   Purpose

   Expression of humanized Mb3Cpf1 and crRNA

   Depositor

   Feng Zhang

   Insert

   huMb3Cpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pY127 (pcDNA3.1-U6-huTsCpf1)

   Plasmid

   #92303

   Purpose

   Expression of humanized TsCpf1 and crRNA

   Depositor

   Feng Zhang

   Insert

   huTsCpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMG005_mNeonGreen_P2A

   Plasmid

   #237215

   Purpose

   Fluorescent reporter codon optimized for Dictyostelid species

   Depositor

   Jordi van Gestel

   Insert

   mNeonGreen-P2A
   

   Use

   Cloning

   Tags

   3xFLAG-tag, HA-tag, SV40-NLS, and nuleoplasmin NL…

   Expression

   Mutation

   Promoter

   Available since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMG008_mCherry_P2A

   Plasmid

   #237216

   Purpose

   Fluorescent reporter codon optimized for Dictyostelid species

   Depositor

   Jordi van Gestel

   Insert

   mCherry-P2A
   

   Use

   Cloning

   Tags

   3xFLAG-tag, HA-tag, SV40-NLS, and nuleoplasmin NL…

   Expression

   Mutation

   Promoter

   Available since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG-T3-hCAS-pA

   Plasmid

   #48625

   Purpose

   Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Kunihiko Naito, Wataru Fujii

   Article

   Fujii et al Nucleic Acids Res. 2013 Aug 30.

   Insert

   Codon optimized Cas9
   

   Use

   CRISPR

   Tags

   FLAG, NLS, and polyA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TC512

   Plasmid

   #122615

   Purpose

   Pmig-13::Zip(-)::QFAD::NLS::SL2::mCherry

   Depositor

   Tian Chi

   Article

   Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

   Insert

   Zip(-)::QFAD::NLS::SL2::mCherry
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNB695

    Plasmid

    #60144

    Purpose

    Disintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    GrLuc
    

    Use

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    Dec. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNB697

    Plasmid

    #60146

    Purpose

    Disintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    RdLuc
    

    Use

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    Dec. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNB693

    Plasmid

    #60145

    Purpose

    Disintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    YeLuc
    

    Use

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    Dec. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pROF346

    Plasmid

    #155331

    Purpose

    Binary vector for expression of PULSE system (under the control of AtUbi10 promoter) controlling Venus-H2B. It constitutively expresses Cerulean-NLS and a plant selection cassette.

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    RB_KanR_Tnos-NLS-PIF6-E-PUbi10_Tnos-nCerulean-PUbi10_Tnos-NLS-VP16-PhyB-PUbi10_Tnos-EL222-NLS-SRDX-PUbi10_T35S-nVenus-POpto_LB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRD443

    Plasmid

    #168469

    Purpose

    For the insertion pf NLS-mScarlet-I-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS-mScarlet-I-H-NSdbd-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-PE7-SB2

    Plasmid

    #228868

    Purpose

    PE7 connected to MLH1-SB and MLH1-SB-NLS via 2A sequence

    Depositor

    Sangsu Bae

    Insert

    PE7-2A-MLH1-SB-NLS-2A-MLH1-SB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PX404 Campylobacter jejuni Cas9

    Plasmid

    #68338

    Purpose

    Campylobacter jejuni Cas9

    Depositor

    Feng Zhang

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    HA-NLS and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDSP33

    Plasmid

    #199375

    Purpose

    Encodes dual cistronic nuclear localized jGCaMP7f and mScarlet-I optimized for C. elegans expression

    Depositor

    Hang Lu

    Insert

    NLS::jGCaMP7f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    no mutations, except added subcellular localizati…

    Promoter

    Available since

    April 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pDSP32

    Plasmid

    #199374

    Purpose

    Encodes dual cistronic nuclear localized jGCaMP7s and mScarlet-I optimized for C. elegans expression

    Depositor

    Hang Lu

    Insert

    NLS::jGCaMP7s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    no mutations, except added subcellular localizati…

    Promoter

    Available since

    April 25, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. BII-gR-PnTW

    Plasmid

    #133356

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS

    Depositor

    Blair Madison

    Insert

    tdTomato-NLS
    

    Use

    Tags

    HA-tagged tdTomato

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDSP31

    Plasmid

    #199373

    Purpose

    Encodes dual cistronic nuclear localized GCaMP6f and mScarlet-I optimized for C. elegans expression

    Depositor

    Hang Lu

    Insert

    NLS::GCaMP6f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    no mutations, except added subcellular localizati…

    Promoter

    Available since

    April 25, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge