We narrowed to 1,112 results for: ENDO 2

1. JDW 858 (pTol2-Kdrl-V5-mScarlet-KRAS4A-WT--AC-Cerulean)

   Plasmid

   #180468

   Purpose

   kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A with an alpha-crystallin mCerulean reporter flanked by Tol2 sites

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   KRAS4A (KRAS Human)
   

   Use

   Zebrafish transgenesis

   Tags

   V5-mScarlet-I

   Expression

   Mutation

   Promoter

   kdrl

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)

   Plasmid

   #156414

   Purpose

   kdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4B-G12V (KRAS Human)
   

   Use

   Zebrafish transgenesis

   Tags

   EGFP

   Expression

   Mutation

   mutant KRAS4B-G12V

   Promoter

   kdrl

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-nSpRY(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES395)

   Plasmid

   #185489

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage nSpRY(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

   Depositor

   Raul Perez-Jimenez, Benjamin Kleinstiver

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage nSpRY(H840A)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-eGFP

   Expression

   Mammalian

   Mutation

   H840A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N13…

   Promoter

   CMV and T7

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

   Plasmid

   #86843

   Purpose

   HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.

   Depositor

   Thomas Cech

   Article

   Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d

   Insert

   FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
   

   Use

   Tags

   FLAG-HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous TERT promoter

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. p.UTA.2.0 miR-27a-3p

   Plasmid

   #82438

   Purpose

   Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p

   Depositor

   Jens Gruber

   Article

   Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

   Insert

   hsa-miR-27a-3p (MIR27A Human)
   

   Use

   Tags

   Expression

   Mutation

   Three copies hsa-miR-27a-3p Complementary region …

   Promoter

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ8666 pHR-RRM2p-BFP-T2A-N term (1-406) dLbCpf1-EE12RR345L-EFS-Zeo-WPRE

   Plasmid

   #140228

   Purpose

   RRM2p-driven expression of BFP and N terminal dCpf1 (Cas12a) 406-split half with leucine zipper. Constitutive EFS-driven zeocin resistance.

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   dLbCpf1 (Cas12a) N terminal half (406) with zipper
   

   Use

   Lentiviral

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   RRM2 endogenous promoter

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)

   Plasmid

   #229819

   Purpose

   A gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Type

   Empty backbone

   Use

   Tol2 destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. ER-CanlonicSF

   Plasmid

   #178343

   Purpose

   To explore endoplasmic reticulum lactate dynamics

   Depositor

   Alejandro San Martín

   Article

   Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

   Insert

   ER-CanlonicSF
   

   Use

   Tags

   ER Retention Signal, ER Signal Exon 1, and ER Sig…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. p.UTA.2.0 miR-27a-3p 3 perfect copies.

   Plasmid

   #82439

   Purpose

   Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p in three copies

   Depositor

   Jens Gruber

   Article

   Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

   Insert

   hsa-miR-27a-3p (MIR27A Human)
   

   Use

   Tags

   Expression

   Mutation

   Three copies hsa-miR-27a-3p Complementary region …

   Promoter

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTol2-DEST-hsp70-zCreI-BFP (JDW 1184)

    Plasmid

    #229831

    Purpose

    A gateway compatible Tol2 destination vector containing the hsp70 promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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