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pFR-mTq2-antiFRET-P2A-K-GECO1

Plasmid

#191469

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) K-GECO1 to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

K-GECO1

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-R-GECO1

Plasmid

#191471

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) R-GECO1 to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

R-GECO1

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 sf:bleach.3

Plasmid

#191925

Purpose

Encodes a GFP design, sf:bleach.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:bleach.3

Tags

His Tag

Expression

Bacterial

Mutation

S72A V112I Y145M T167V H181I V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 bleach.3

Plasmid

#191914

Purpose

Encodes a GFP design, bleach.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

bleach.3

Tags

His Tag

Expression

Bacterial

Mutation

S72A V112I Y145M T167V H181I V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 mid.12

Plasmid

#191913

Purpose

Encodes a GFP design, mid.12

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.12

Tags

His Tag

Expression

Bacterial

Mutation

T65S S72V Y145M T167V H181I V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 em-blue-shift.2

Plasmid

#191901

Purpose

Encodes a GFP design, em-blue-shift.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

em-blue-shift.2

Tags

His Tag

Expression

Bacterial

Mutation

T65S S72A T108I Y145F V150I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 acid.5

Plasmid

#191903

Purpose

Encodes a GFP design, acid.5

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

acid.5

Tags

His Tag

Expression

Bacterial

Mutation

S72T Y145M T167V H181V

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 acid.4

Plasmid

#191898

Purpose

Encodes a GFP design, acid.4

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

acid.4

Tags

His Tag

Expression

Bacterial

Mutation

V61L Q69L Y145M L220V

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 acid.3

Plasmid

#191897

Purpose

Encodes a GFP design, acid.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

acid.3

Tags

His Tag

Expression

Bacterial

Mutation

T65S Q69L S72A T108V Y145M V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 slow.2

Plasmid

#191894

Purpose

Encodes a GFP design, slow.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

slow.2

Tags

His Tag

Expression

Bacterial

Mutation

V68M Q69L S72T V112I Y145M T167V L220V V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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