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pBS Coupler 1

Plasmid

#185824

Purpose

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Depositor

Atsushi Miyawaki

Article

Shimozono et al Methods Cell Biol. 2008;85:381-93. doi:

Insert

(GlyGlyGlyGlySer)x3 linker

Use

Cloning vector

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCDW114

Plasmid

#70061

Purpose

artificial transcription template "wild-type"

Depositor

Jeff Gelles

Article

Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

Insert

lambda PR' - repeat cassette - E. coli rpoB - lambda TR'

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only
HIV-1 integrase W235F in pET-15b

Plasmid

#61681

Purpose

HIV-1 integrase W235F in pET-15b

Depositor

Robert Craigie

Article

Li et al EMBO J. 2006 Mar 22;25(6):1295-304. Epub

Insert

HIV-1 integrase W235F

Tags

His

Mutation

W235F

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
pKG326

Plasmid

#69753

Purpose

Retroviral expression of CENP-T aa 1-531

Depositor

Iain Cheeseman

Insert

CENP-T aa 1-531 (CENPT Human)

Use

Retroviral

Tags

eGFP-TEV-S

Expression

Mammalian

Mutation

E.coli optimized sequence; A216T and G459R mutati…

Available Since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only
pCDW115

Plasmid

#70062

Purpose

artificial transcription template "pause ablation"

Depositor

Jeff Gelles

Article

Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

Insert

lambda PR' A+2G T+6G - repeat cassette - E. coli rpoB - lambda TR'

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only
pDONR221 Spb1

Plasmid

#63777

Purpose

yeast ORF Spb1 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Spb1

Use

Gateway donor vector

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221 Rrp8

Plasmid

#63775

Purpose

yeast ORF Rrp8 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Rrp8

Use

Gateway donor vector

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pKeratin-PSmOrange2

Plasmid

#34962

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2012 Aug 17.

Insert

PSmOrange2

Tags

Keratin

Expression

Mammalian

Mutation

R17H, R36H, F64I, Q194L, A224S, G226A (mutations …

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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peZinCh-1

Plasmid

#24172

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eZinCh-1

Expression

Mammalian

Mutation

Cerulean and Citrine both contain mutations Y39H …

Promoter

CMV

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pIC145

Plasmid

#63068

Purpose

Retroviral expression of EGFP-TEV-S-tag CENP-O for protein localization and affinity purification (LAP)

Depositor

Iain Cheeseman

Insert

CENP-O, IMAGE clone 3942844 (CENPO Human)

Use

Retroviral

Tags

EGFP and TEV-S

Expression

Mammalian

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pAV2021

Plasmid

#37842

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

Phsp-4GFP

Expression

Worm

Promoter

hsp-4

Available Since

Dec. 7, 2012

Availability

Academic Institutions and Nonprofits only
pMpGWB306-MpRTN1

Plasmid

#228476

Purpose

Binary vector for the expression of MpRTN1 in plants

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

MpRTN1

Expression

Bacterial and Plant

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.SLC17A1-flag

Plasmid

#224418

Purpose

Express SLC17A1 in mammalian cells

Depositor

Jonathan Long

Insert

Solute carrier family 17 (sodium phosphate), member 1 (SLC17A1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCEF-FKBP12-Bla2

Plasmid

#190946

Purpose

Protein fragment complementation

Depositor

Jakob Reiser

Article

Ou et al Gene Ther. 2013 Jan;20(1):43-50. doi: 10

Insert

C-terminal fragment of BLAK fused to FKBP12

Expression

Mammalian

Promoter

CEF

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pMA5096

Plasmid

#184850

Purpose

General purpose retroviral cloning vector, hygromycin resistance

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Type

Empty backbone

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBS Coupler 6

Plasmid

#185829

Purpose

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Depositor

Atsushi Miyawaki

Article

Shimozono et al Methods Cell Biol. 2008;85:381-93. doi:

Insert

(GlyGlyGlyGlySer)x3 linker

Use

Cloning vector

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS Coupler 5

Plasmid

#185828

Purpose

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Depositor

Atsushi Miyawaki

Article

Shimozono et al Methods Cell Biol. 2008;85:381-93. doi:

Insert

(GlyGlyGlyGlySer)x3 linker

Use

Cloning vector

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS Coupler 4

Plasmid

#185827

Purpose

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Depositor

Atsushi Miyawaki

Article

Shimozono et al Methods Cell Biol. 2008;85:381-93. doi:

Insert

(GlyGlyGlyGlySer)x3 linker

Use

Cloning vector

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS Coupler 2

Plasmid

#185825

Purpose

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Depositor

Atsushi Miyawaki

Article

Shimozono et al Methods Cell Biol. 2008;85:381-93. doi:

Insert

(GlyGlyGlyGlySer)x3 linker

Use

Cloning vector

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS Coupler 3

Plasmid

#185826

Purpose

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Depositor

Atsushi Miyawaki

Article

Shimozono et al Methods Cell Biol. 2008;85:381-93. doi:

Insert

(GlyGlyGlyGlySer)x3 linker

Use

Cloning vector

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDK365

Plasmid

#134195

Purpose

Retroviral plasmid with SKAP insert

Depositor

Iain Cheeseman

Insert

SKAP Alternate Start- LAP (with G5 hardeneded) (KNSTRN Human)

Use

Retroviral

Tags

LAP (GFP)

Expression

Mammalian

Mutation

Alternate Start, G5 siRNA hardened

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221 Rpf1

Plasmid

#63771

Purpose

yeast ORF Rpf1 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Rpf1

Use

Gateway donor vector

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 Rrp1

Plasmid

#63773

Purpose

yeast ORF Rrp1 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Rrp1

Use

Gateway donor vector

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 Rsa4

Plasmid

#63776

Purpose

yeast ORF Rsa4 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Rsa4

Use

Gateway donor vector

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 Ssf1

Plasmid

#63778

Purpose

yeast ORF Ssf1 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Ssf1

Use

Gateway donor vector

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 Drs1

Plasmid

#63744

Purpose

yeast ORF Drs1 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Drs1

Use

Gateway donor vector

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 Fpr3

Plasmid

#63745

Purpose

yeast ORF Fpr3 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Fpr3

Use

Gateway donor vector

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 Mak11

Plasmid

#63761

Purpose

yeast ORF Mak11 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Mak11

Use

Gateway donor vector

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 Mak16

Plasmid

#63762

Purpose

yeast ORF Mak16 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Mak16

Use

Gateway donor vector

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 Nip7

Plasmid

#63763

Purpose

yeast ORF Nip7 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Nip7

Use

Gateway donor vector

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge