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We narrowed to 2,182 results for: Xpa

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Showing: 2161 - 2180 of 2182 results

  2. pCMV_FLAG-NFL(K363TAG)

    Plasmid
    #182661
    Purpose
    Expresses mouse neurofilament light chain with a TAG codon at the position K363 & an N-terminal FLAG tag (DYKDDDDK) in mammalian cells. Can be used for genetic code expansion & click labeling of NFL.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse neurofilament light chain with a K363TAG mutation (Nefl Mouse)
    Tags
    FLAG tag (DYKDDDDK)
    Expression
    Mammalian
    Mutation
    K363TAG
    Promoter
    CMV
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182661-plasmid-map-sequence-id-363012

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  3. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid
    #182287
    Purpose
    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182287-plasmid-map-sequence-id-363047

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  4. pAcBac2.tR4-OMeYRS/GFP*

    Plasmid
    #50831
    Purpose
    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells
    Depositor
    Peter Schultz
    Article
    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11
    Inserts
    two-copy Tyr tRNA cassette
    EGFP*
    OMeYRS
    two copy Tyr tRNA cassette
    Use
    Baculoviral
    Tags
    His, Myc-6xHis, and WPRE
    Expression
    Insect
    Mutation
    Y39TAG and able to charge various unnatural amino…
    Promoter
    CAG, CMV, and U6 and H1
    Available Since
    June 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    50831-plasmid-map-sequence-id-193569

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  5. pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A

    Plasmid
    #154773
    Purpose
    plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Y125A in G1PylS, hybrid PylT with A41AA and C55A …
    Promoter
    EF1
    Available Since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154773-plasmid-map-sequence-id-310428

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  6. pCDF-Mm2

    Plasmid
    #197100
    Purpose
    To express the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA and allow UAG codon to be translated into these derivati
    Depositor
    Kensaku Sakamoto
    Article
    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20
    Insert
    PylRS variant, Pyl tRNA, kanamycin resistance gene
    Expression
    Bacterial
    Mutation
    Lys61, Glu131, Ala306, Phe384, Glu444 (PylRS)
    Available Since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    197100-plasmid-map-sequence-id-393189

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  9. pAcBac1-Mb-Fluoro-Phe D6

    Plasmid
    #197577
    Purpose
    Expression of Methanosarcina barkeri (Mb) Fluoro-Phe D6 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10
    Inserts
    Mb Pyl Fluoro-Phe B5 synthetase
    Pyl-tRNA (4 copies)
    Tags
    Nuclear export sequence + FLAG
    Expression
    Mammalian
    Mutation
    N311A, C313M, V366G, W382T
    Promoter
    CMV and U6/H1
    Available Since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197577-plasmid-map-sequence-id-388240

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  10. pAcBac1-Mb-Fluoro-Phe B5

    Plasmid
    #197576
    Purpose
    Expression of Methanosarcina barkeri (Mb) Fluoro-Phe B5 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10
    Inserts
    Mb Pyl Fluoro-Phe B5 synthetase
    Pyl-tRNA (4 copies)
    Tags
    Nuclear export sequence + FLAG
    Expression
    Mammalian
    Mutation
    N311G, C313A
    Promoter
    CMV and U6/H1
    Available Since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197576-plasmid-map-sequence-id-388235

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  11. pERM2-nhpSer

    Plasmid
    #201922
    Purpose
    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.
    Depositor
    Richard Cooley, Ryan Mehl
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    Tags
    None
    Expression
    Bacterial
    Mutation
    E412P, E414F, T417K, P495M, I496W, F529S and H67R…
    Promoter
    GlnS (constitutive), OXB20 (constitutive), lpp (c…
    Available Since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201922-plasmid-map-sequence-id-395413

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  12. pEVOL-pAzF[TAG]

    Plasmid
    #164579
    Purpose
    Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons
    Depositor
    Ryan Mehl
    Article
    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2
    Inserts
    Mj pCNFRS[TAG] (aaRS1)
    Mj pCNFRS[TAG] (aaRS2)
    Mj tRNA[TAG]
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    araBAD, glnS, and proK
    Available Since
    May 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    164579-plasmid-map-sequence-id-392918

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  13. pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF)

    Plasmid
    #182652
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & 4x M15 tRNACUA used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    4xU6-tRNAM15
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available Since
    May 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182652-plasmid-map-sequence-id-440860

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  14. pDule2-3-nitroTyrosine (A7)

    Plasmid
    #174079
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32H H70T D158H I159A L162R
    Promoter
    GlnRS (constitutive)
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174079-plasmid-map-sequence-id-341130

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  15. pAcBac1-Ma-Acridone-RS1 (A7)

    Plasmid
    #197566
    Purpose
    Expression of Methanomethylophilus alvus (Ma) Acridone RS1 (A7) tRNA synthetase/Pyl-tRNA pair for the encoding of Acridone at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    M. alvus PylRS - Acd RS1 (A7)
    M. alvus Pyl-tRNA (6) (4x copies)
    Tags
    None
    Expression
    Mammalian
    Mutation
    N166A, V168G, W239C
    Promoter
    CMV and U6/H1
    Available Since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197566-plasmid-map-sequence-id-388228

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  16. pDule-3-nitroTyrosine (A7)

    Plasmid
    #174078
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32H H70T D158H I159A L162R
    Promoter
    GlnRS (constitutive)
    Available Since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174078-plasmid-map-sequence-id-345712

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  17. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid
    #182653
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    Tags
    HA tag and nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    E55D and Y306A/Y384F (AF) double mutant of Methan…
    Promoter
    CMV and U6
    Available Since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    182653-plasmid-map-sequence-id-363026

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  18. EF1a-L274GMmMetRS-T2A-mCherry

    Plasmid
    #220800
    Purpose
    Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance
    Depositor
    Elizabeth Kirby
    Article
    Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18
    Insert
    Mars1 (Mars1 Mouse)
    Use
    Lentiviral
    Tags
    2xFLAG and T2A-mCherry
    Mutation
    mutation in MetRS to change aa 274 from L to G
    Promoter
    EF1a
    Available Since
    June 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220800-plasmid-map-sequence-id-437285

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  19. pPaGE Pyl TAG FliC T248TAG

    Plasmid
    #160089
    Purpose
    Encodes for MmPyl tRNA synthetase/tRNA pair and for flagellin protein (FliC) with TAG mutation at site 248, for unnatural amino acid incorporation into the flagellin protein in Pseudomonas aeruginosa.
    Depositor
    Lital Alfonta
    Article
    Ozer et al Sci Adv. 2021 Jun 11;7(24). pii: 7/24/ea
    Inserts
    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Flagellin
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Changed Threonine 248 to TAG stop-codon.
    Promoter
    Pseudomonas aeruginosa Leu-tRNA native promoter a…
    Available Since
    June 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160089-plasmid-map-sequence-id-314779

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Showing: 2161 - 2180 of 2182 results