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Plasmid#193374PurposeContains the third TALE repeat harboring RV as RVDDepositorInsertRepeat 3 RV
UseTALENTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p4RV
Plasmid#193375PurposeContains the fourth TALE repeat harboring RV as RVDDepositorInsertRepeat 4 RV
UseTALENTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXZ13lacZalphaKanR
Plasmid#160230PurposeLike pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJL31
Plasmid#227428PurposeKanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)DepositorInsertmWasabi
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL32
Plasmid#227429PurposeKanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)DepositorInserttdTomato
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL33
Plasmid#227430PurposeKanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)DepositorInsertmWasabi
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL34
Plasmid#227431PurposeKanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)DepositorInserttdTomato
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
Plasmid#219687PurposeLevel 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ub-GyrA
Plasmid#128737PurposeExpresses Ub fused to GyrA intein at the C-terminalDepositorInsertUBC
UseTagsMxe GyrAExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYTK077
Plasmid#65184PurposeEncodes KanamycinR as a Type 6 part to be used in the Dueber YTK systemDepositorInsertKanamycinR
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMulti_K_ccdB
Plasmid#223852PurposeGEC-acceptor for 4G-cloning, designed for expression in E. coli (Kanamyicin resistance)DepositorTypeEmpty backboneUseGolden-gate acceptorTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1T11 - T2BP/p35S (BFP, mCherry, NeonGreen)
Plasmid#219688PurposeLevel 1 History-dependent target, switch Bxb1 then PhiC31, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2BP/p35S (BFP, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1T9 - T2PB/p35S (mTagBFP2, mCherry, NeonGreen)
Plasmid#219686PurposeLevel 1 History-dependent target, switch PhiC31 then Bxb1, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PB/p35S (mTagBFP2, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1656)
Plasmid#160618PurposeModule for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).DepositorInsertTnos:NptII:Pnos-SF-P35s:hCas9:tNos
UseCRISPRTagsExpressionMutationBsaI and BsmBI sites removedPromoterPnos, 35SAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGGPK-AG2
Plasmid#222027PurposeEmpty Kanamycin resistant GreenGate destination vector based on pGGP-AG.DepositorTypeEmpty backboneUseSynthetic Biology; Greengate compatible cloning v…TagsExpressionMutationPromoterAvailable sinceDec. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
L1T18 - T2PsB/p35S preswitched (mTagBFP2, mCherry, NeonGreen)
Plasmid#219690PurposeLevel 1 preswitched History-dependent target, switch with Bxb1, p35S promoter, and output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PsB/p35S (mTagBFP2, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
nTALECD for_TUB4S351F_1397NC
Plasmid#193382PurposenTALECD (1397NC) expression vectorDepositorInsertFor specific C-to-T base editing to introduce an amino acid mutation in the coding sequence of AtTUB4.
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA BfuAI large stuffer
Plasmid#52628Purposelentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiencyDepositorInsertKanamycin cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEPkan-S2
Plasmid#61601PurposePCR template for en passant (two-step Red-mediated) mutagenesisDepositorInsertKana_I-SceI
UseTagsExpressionMutationPromoterAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJV62
Plasmid#26910DepositorInsertChe9c gene 61
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEPkan-S
Plasmid#41017PurposePCR template for en passant (two-step Red-mediated) mutagenesisDepositorInsertKana_I-SceI
UseTagsExpressionMutationPromoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTK204
Plasmid#13534DepositorInsertFLIPW-CTYT
UseTagsSee mapExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSC27
Plasmid#117478Purposemobilizable, SRB-stable, kanamycin-resistant cloning vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK1056
Plasmid#209546PurposeBTK Type 4 antibiotic resistance cassette for Golden Gate assemblyDepositorInsertType 4 Kanamycin resistance cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 12, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTK164
Plasmid#13533DepositorInsertFLIPW-CTY
UseTagsECFP and VenusExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTK203
Plasmid#13536DepositorInsertFLIPW-TCTY
UseTagsSee mapExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
GB1-GyrA
Plasmid#128738PurposeExpresses GB1 fused to GyrA intein at the C-terminalDepositorInsertGB1
UseTagsGyrAExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVP311
Plasmid#222070PurposeMultiGreen 2.0 AB level 1 acceptor vector, kanamycin selection.DepositorTypeEmpty backboneUseSynthetic Biology; Greengate compatible cloning v…TagsExpressionMutationAll unused Esp3i sites are domesticatedPromoterAvailable sinceDec. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVP312
Plasmid#222071PurposeMultiGreen 2.0 BC level 1 acceptor vector, kanamycin selection.DepositorTypeEmpty backboneUseSynthetic Biology; Greengate compatible cloning v…TagsExpressionMutationAll unused Esp3i sites are domesticatedPromoterAvailable sinceDec. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVP313
Plasmid#222072PurposeMultiGreen 2.0 CD level 1 acceptor vector, kanamycin selection.DepositorTypeEmpty backboneUseSynthetic Biology; Greengate compatible cloning v…TagsExpressionMutationAll unused Esp3i sites are domesticatedPromoterAvailable sinceDec. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits