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  1. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...signals when used with the same protein (Hayashi-Takanaka et al., 2014). Other dyes may prove challenging...and reliable imaging and assay results (Hayashi-Takanaka et al., 2014)​​. Conjugates with lower DOL tend...intracellular fluorescence live imaging ​​(Hayashi-Takanaka et al., 2014). Dyes can also vary in their brightness...994. https://doi.org/10.1038/nmeth.4403 Hayashi-Takanaka, Y., Stasevich, T. J., Kurumizaka, H., Nozaki,...

  3. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ... for transformations. Con: Expensive.   Kanamycin Kanamycin, or kan, is an aminoglycoside which inhibits...decorative" title="Check icon" %} Satellite colonies Kanamycin Excellent No Bacteria and eukaryotes (G418) {%...

  4. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...were first expressed in E. coli in the 1980s  (Kanabrocki et al 1986). Much later, it was shown that putting...her on Twitter @jessicapolka.     References 1. Kanabrocki, J. A., Quackenbush, R. L. & Pond, F. R. Organization...

  5. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...NGG) 42875 pCRISPR BsaI E. coli, S. pneumoniae Kanamycin none, need Cas9 plasmid Marraffini 42876 pCas9...

  6. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...serotype can affect spread from the injection site  (Kanaan et al., 2017), axonal transport  (Murlidharan et...5), 1–41. https://doi.org/10.3390/cells12050785 Kanaan, N. M., Sellnow, R. C., Boye, S. L., Coberly, B...

  8. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ... the B. subtilis Single Gene Deletion Library—Kanamycin and the CRISPRi Essential gene Knockdown library...regions (excluding start and stop codons) with kanamycin cassettes and were carefully designed to allow...

  12. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...contains a nonfunctional truncated version of the kanamycin resistance gene (missing the first two codons)...pUCXKT will therefore have resistance to both kanamycin and ampicillin (from the pUC19 backbone). *False...
Showing: 1 - 20 of 26 results