We narrowed to 28 results for: kana
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TypeBlog Post...signals when used with the same protein (Hayashi-Takanaka et al., 2014). Other dyes may prove challenging...and reliable imaging and assay results (Hayashi-Takanaka et al., 2014). Conjugates with lower DOL tend...intracellular fluorescence live imaging (Hayashi-Takanaka et al., 2014). Dyes can also vary in their brightness...994. https://doi.org/10.1038/nmeth.4403 Hayashi-Takanaka, Y., Stasevich, T. J., Kurumizaka, H., Nozaki,...
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Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog Post...backbone contains a kanamycin resistance cassette, you would plate the cells on kanamycin-containing agar..... Only cells carrying plasmids that contain a kanamycin resistance cassette will grow. However, often ... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog Post... for transformations. Con: Expensive. Kanamycin Kanamycin, or kan, is an aminoglycoside which inhibits...decorative" title="Check icon" %} Satellite colonies Kanamycin Excellent No Bacteria and eukaryotes (G418) {%... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog Post...were first expressed in E. coli in the 1980s (Kanabrocki et al 1986). Much later, it was shown that putting...her on Twitter @jessicapolka. References 1. Kanabrocki, J. A., Quackenbush, R. L. & Pond, F. R. Organization... -
CRISPR Plasmids - Bacteria
TypeCollection...NGG) 42875 pCRISPR BsaI E. coli, S. pneumoniae Kanamycin none, need Cas9 plasmid Marraffini 42876 pCas9... -
University of Florida Serotype Testing Panel for the Eye and Brain
TypeCollection...4215-4231. PMID: 26865709 Other citations include: Kanaan, et al. 2017. Rationally Engineered AAV Capsids... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog Post...serotype can affect spread from the injection site (Kanaan et al., 2017), axonal transport (Murlidharan et...5), 1–41. https://doi.org/10.3390/cells12050785 Kanaan, N. M., Sellnow, R. C., Boye, S. L., Coberly, B... -
15 Hot Plasmids from 2017
TypeBlog Post... the B. subtilis Single Gene Deletion Library—Kanamycin and the CRISPRi Essential gene Knockdown library...regions (excluding start and stop codons) with kanamycin cassettes and were carefully designed to allow... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog Post...functional in a variety of plant species. The kanamycin antibiotic resistance gene is controlled by a ... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post...Voojis M, Beverloo HB, Al Allak B, van Drunen E, Kanaar R, Berns A, Jonkers J. Proc Natl Acad Sci USA. ... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post...contains a nonfunctional truncated version of the kanamycin resistance gene (missing the first two codons)...pUCXKT will therefore have resistance to both kanamycin and ampicillin (from the pUC19 backbone). *False... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post...Class Mode of Action* Working Concentration** Kanamycin aminoglycoside Binds 30S ribosomal subunit; ... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog Post...resistant plasmid, pSC101, and a newly developed kanamycin resistant plasmid, pSC102, with EcoRI and selected... -
The Importance of High Titer for AAV Transductions
TypeBlog Post...remeasure your titer after diluting to confirm. Luke Makana Hanley is a Research Team Lab Tech at Addgene working... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post...chloramphenicol, erythromicin, gentamicin, hygromycin, kanamycin, nourseothricin (clonNat), spectinomycin, streptomycin... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post...K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, Fan M (2020... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog Post...K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, Fan M (2020... -
Tips for arabidopsis transformation
TypeBlog Post... plant selection markers (basta, hygromycin, kananamycin, tunicamycin) (Nakagawa et al., 2007). Alternatively... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...majority of plasmids contain either an ampicillin or kanamycin resistance gene. Every step along the way involves... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog Post...resistance gene as the plant-selectable marker and a kanamycin resistance gene for bacterial selection. The process...