We narrowed to 5,416 results for: KRAS

1. pLVPRT-tTR-KRAB

   Plasmid

   #11648

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hPrion promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   hPrion, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. 2_T7-mScarlet3-KRas

   Plasmid

   #225929

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras membrane localisation signal

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   mScarlet3-KRas
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#2

   Plasmid

   #172984

   Purpose

   CRISPRi for TAOK2

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   sgTAOK2i (TAOK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCX-SpiCee-Kras

   Plasmid

   #140838

   Purpose

   mammalian expression of SpiCee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   SpiCee-Kras
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1+FLAG-KrasnatG12D

   Plasmid

   #206842

   Purpose

   To express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag.

   Depositor

   Christopher Counter

   Article

   Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

   Insert

   Kras (Kras Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Glycine 12 to Aspartic Acid

   Promoter

   Available since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-YFP-KRAS4B

   Plasmid

   #112718

   Purpose

   Expresses YFP-KRAS4B fusion protein used for FRET studies in eukaryotic cells.

   Depositor

   Kenneth Westover

   Article

   Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

   Insert

   YFP-KRAS4B (KRAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVPT-rtTR-KRAB

   Plasmid

   #11777

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   mPGK, GFP, rtTR-KRAB, Tet-off
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVUTHshGATA1-tTR-KRAB

   Plasmid

   #11650

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   hUbiquitin C, GFP, tTR-KRAB, shRNA against GATA1, Tet-on (GATA1 Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. (364) SB KRAB-SadCas9

   Plasmid

   #163022

   Purpose

   Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   Transposon

   Tags

   KRAB and myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   TET-On

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR2.1-KRAS-G12V

    Plasmid

    #129091

    Purpose

    KI KRAS G12V mutation into human KRAS locus

    Depositor

    Toshiro Sato

    Article

    Matano et al Nat Med. 2015 Mar;21(3):256-62. doi: 10.

    Insert

    KRAS(G12V) (KRAS Human)
    

    Use

    Homologous recombination donor plasmid

    Tags

    Expression

    Mutation

    G12V

    Promoter

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCLXEBR-pTF-kRasV12

    Plasmid

    #114318

    Purpose

    TET-inducible expression of the V12-mutant form of the ki-Ras protein

    Depositor

    Patrick Salmon

    Insert

    Ras (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G12V oncogenic mutation. Constitutively active K-…

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpRY dCas9 KRAB

    Plasmid

    #188501

    Purpose

    Expresses FLAG tagged SpRY dCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBAD-HisD-KRaION2

    Plasmid

    #177800

    Purpose

    Bacterial expression of green fluorescent potassium indicator KRaION2

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    KRaION2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. NLS-HA-2xMCP-KRAB

    Plasmid

    #126589

    Purpose

    Expresses MCP (MS2 Coat Protein) fusion to KRAB in mammalian cells, lentiviral backbone

    Depositor

    David Segal

    Article

    O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

    Insert

    2XMCP-KRAB
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C promoter

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Dronpa-Kras

    Plasmid

    #182329

    Purpose

    Plasma membrane-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    Dronpa-Kras
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-AktAR-Kras

    Plasmid

    #123357

    Purpose

    FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.

    Depositor

    Jin Zhang

    Article

    Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

    Insert

    AktAR-Kras
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMVTO-KRAS-G12V

    Plasmid

    #179654

    Purpose

    KRAS active mutant (G12V mutation)

    Depositor

    Xiaojing Gao

    Article

    Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

    Insert

    KRAS G12V
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. 3_T7-mScarlet3-KRas6R

    Plasmid

    #225930

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras6R membrane localisation signal

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    mScarlet3-KRas6R
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMVTO-KRAS-G12C

    Plasmid

    #179655

    Purpose

    KRAS active mutant (G12C mutation)

    Depositor

    Xiaojing Gao

    Article

    Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

    Insert

    KRAS G12C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G12C

    Promoter

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. HiFi dCas9 KRAB

    Plasmid

    #188500

    Purpose

    Expresses FLAG tagged HiFi dCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/R691A/H840A

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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