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AAVS1-Puro cTnT CCND2-T2A-ChloC

Plasmid

#179841

Purpose

donor plasmid for cardiac-specific CCND2-T2A-Chloc expression in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR and TALEN; Donor plasmid

Tags

chloride-conducting ChR (ChloC)

Expression

Mammalian

Mutation

Promoter

Troponin T promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT CCND2-T2A-iChloC

Plasmid

#179842

Purpose

donor plasmid for cardiac-specific CCND2-T2A-iChloc expression in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR and TALEN; Donor plasmid

Tags

improved chloride-conducting ChR (iChloC)

Expression

Mammalian

Mutation

Promoter

Troponin T promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT CCND2-T2A-luciferase

Plasmid

#179844

Purpose

donor plasmid for cardiac-specific CCND2-T2A-luciferase expression in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR, Luciferase, and TALEN ; Donor plasmid

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Troponin T promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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mAzGreen-Cyclin D2-NeoR

Plasmid

#215062

Purpose

Expresses mAzGreen-Cyclin D2 in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND2 (CCND2 Human)

Use

Tags

mAzGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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SS-Cyclin D2

Plasmid

#215115

Purpose

Expresses SS-Cyclin D2 in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND2 (CCND2 Human)

Use

Tags

SS (STREP-STREP)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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mAzGreen-Cyclin D2-PuroR

Plasmid

#215058

Purpose

Expresses mAzGreen-Cyclin D2 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND2 (CCND2 Human)

Use

Retroviral

Tags

mAzGreen

Expression

Mammalian

Mutation

Promoter

LTR

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-Cyclin D2

Plasmid

#215051

Purpose

Expresses EGFP-Cyclin D2 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND2 (CCND2 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

LTR

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-AKT3

Plasmid

#116711

Purpose

Lentiviral expression of AKT3

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AKT3 (AKT3 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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AKT3 gRNA (BRDN0001146868)

Plasmid

#76217

Purpose

3rd generation lentiviral gRNA plasmid targeting human AKT3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AKT3 (AKT3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AKT3 gRNA (BRDN0001144935)

Plasmid

#76218

Purpose

3rd generation lentiviral gRNA plasmid targeting human AKT3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AKT3 (AKT3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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AKT3 gRNA (BRDN0001145071)

Plasmid

#76219

Purpose

3rd generation lentiviral gRNA plasmid targeting human AKT3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AKT3 (AKT3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AKT3 gRNA (BRDN0001147841)

Plasmid

#76220

Purpose

3rd generation lentiviral gRNA plasmid targeting human AKT3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AKT3 (AKT3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1-Puro cTnT CCND2-T2A-ChloC-P2A-eGFP

Plasmid

#179840

Purpose

donor plasmid for cardiac-specific CCND2-T2A-Chloc-P2A-eGFP expression in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR and TALEN; Donor plasmid

Tags

chloride-conducting ChR (ChloC) and eGFP

Expression

Mammalian

Mutation

Promoter

Troponin T promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKT3_WT

Plasmid

#82093

Purpose

Gateway Donor vector containing AKT3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT3 (AKT3 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-AKT3-M339I

Plasmid

#116105

Purpose

Lentiviral expression of AKT3 M339I

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AKT3 (AKT3 Human)

Use

Lentiviral

Tags

Expression

Mutation

M339I

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-AKT3-S370P

Plasmid

#116106

Purpose

Lentiviral expression of AKT3 S370P

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AKT3 (AKT3 Human)

Use

Lentiviral

Tags

Expression

Mutation

S370P

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_AKT3_p.E17K

Plasmid

#82172

Purpose

Gateway Donor vector containing AKT3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT3 (AKT3 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E17K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag AKT3

Plasmid

#20423

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

AKT3 (AKT3 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223-AKT3

Plasmid

#23539

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AKT3 (AKT3 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

S2R

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
8061-E14

Plasmid

#234251

Purpose

Gateway ORF Entry clone of human SMAD9 with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

SMAD9 (SMAD9 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

May 29, 2025

Availability

Academic Institutions and Nonprofits only

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