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Addgene
Showing: 201 - 220 of 4531 results

  1. R777-E111 Hs.IRS2

    Plasmid
    #70395
    Purpose
    Gateway ORF clone of codon optimized human IRS2 [NM_003749] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    IRS2 (IRS2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    70395-plasmid-map-sequence-id-125844

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  2. R777-E139 Hs.NF1

    Plasmid
    #70423
    Purpose
    Gateway ORF clone of codon optimized human NF1 [NM_000267.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    NF1 (NF1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    70423-plasmid-map-sequence-id-125872

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  3. R777-E237 Hs.RASAL3

    Plasmid
    #70521
    Purpose
    Gateway ORF clone of codon optimized human RASAL3 [NM_022904.1] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASAL3 (RASAL3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    70521-plasmid-map-sequence-id-125970

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  4. R777-E238 Hs.RASAL3-nostop

    Plasmid
    #70522
    Purpose
    Gateway ORF clone of codon optimized human RASAL3 [NM_022904.1] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASAL3 (RASAL3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    70522-plasmid-map-sequence-id-125971

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  5. R777-E309 Hs.SHC2

    Plasmid
    #70593
    Purpose
    Gateway ORF clone of codon optimized human SHC2 [NM_012435.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SHC2 (SHC2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    70593-plasmid-map-sequence-id-126042

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  7. hCas9_D10A

    Plasmid
    #41816
    Purpose
    Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering
    Depositor
    George Church
    Article
    Mali et al Science. 2013 Jan 3.
    Insert
    Cas9_D10A
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A nickase
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    41816-plasmid-map-sequence-id-116715

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  14. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

    Plasmid
    #174137
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…
    Promoter
    CMV and T7
    Availability
    Academic Institutions and Nonprofits only
    174137-plasmid-map-sequence-id-341916

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  15. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

    Plasmid
    #174140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…
    Promoter
    CMV and T7
    Availability
    Academic Institutions and Nonprofits only
    174140-plasmid-map-sequence-id-341918

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  16. pCS2+/N-SNAPf

    Plasmid
    #184415
    Purpose
    vector backbone to clone insert downstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis
    Depositor
    Ann Miller
    Article
    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do
    Type
    Empty backbone
    Use
    For mrna synthesis
    Tags
    SNAPf-tag
    Expression
    Mutation
    Codon optimized for expression in Xenopus laevis …
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    184415-plasmid-map-sequence-id-365684

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  17. pCS2+/C-SNAPf

    Plasmid
    #184416
    Purpose
    vector backbone to clone insert upstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis
    Depositor
    Ann Miller
    Article
    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do
    Type
    Empty backbone
    Use
    For mrna synthesis
    Tags
    SNAPf-tag
    Expression
    Mutation
    Codon optimized for expression in Xenopus laevis …
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    184416-plasmid-map-sequence-id-362158

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  18. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213029
    Purpose
    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Insert
    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    213029-plasmid-map-sequence-id-422050

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Showing: 201 - 220 of 4531 results