We narrowed to 7,151 results for: CIT

1. pDestTol2-actinb-kcnk9-IRES-EGFP

   Plasmid

   #164949

   Purpose

   Tol2 construct: actinb (actb2) promoter driven zebrafish kcnk9 and EGFP

   Depositor

   GuangJun Zhang

   Article

   Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

   Insert

   kcnk9 (kcnk9 Zebrafish)
   

   Use

   Tol2 transposon destination vector

   Tags

   IRES-EGFP

   Expression

   Mutation

   Promoter

   actinb (actb2) promoter

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLEX307-HCoV-NL63-3CL

   Plasmid

   #160286

   Purpose

   Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV hSyn FLEx-OFF Kir2.1-2A-GFP

   Plasmid

   #161580

   Purpose

   Express Kir2.1 in cells not expressing Cre.

   Depositor

   Kevin Beier, Robert Malenka

   Article

   Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

   Insert

   Kir2.1-2A-GFP (Kcnj2 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEX307-Bat-CoV-HKU9-3CL

   Plasmid

   #160284

   Purpose

   Allows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEF073 MBP-Axin-His

   Plasmid

   #154011

   Purpose

   Expresses MBP-Axin-His (human Axin as a fusion protein with MBP and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   Axin1 (AXIN1 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. 416Gal-FUS-R521C-YFP

   Plasmid

   #29622

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   R521C

   Promoter

   Available since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pLEX307-SARS-CoV-3CL

   Plasmid

   #160280

   Purpose

   Allows for constituitive mammalian expression of the SARS-CoV 3CL protease via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. xCas12i

   Plasmid

   #195333

   Purpose

   vector for encoding a human codon-optimized xCas12i driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

   Insert

   human codon-optimized xCas12i
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CBh, CMV, hU6

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLC-CUL4A K705R-P2A-Puro

   Plasmid

   #124305

   Purpose

   Lentiviral vector for expression of Nedd8 attachment mutant K705R CUL4A-P2A-Puro casette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   CUL4A (CUL4A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   changed Lysine 705 to Arginine

   Promoter

   CMV

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGAL4-DBD-CEBPa-WT

    Plasmid

    #215601

    Purpose

    For overexpression of Gal4-DBD CEBPa WT

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    CEBPa (CEBPA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. FCK-Chronos-GFP

    Plasmid

    #80902

    Purpose

    Lenti-mediated expression of Chronos-GFP under the CaMKII promoter.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUPD2 sgRNAscaffold 2.1 scRNA (GB1437)

    Plasmid

    #160571

    Purpose

    Version of SgRNA scaffold with the sequence 2.1 of Ms2 aptamer in the 3' end.

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    sgRNAscaffold 2.1 scRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA 3.2-FUS-1-413aa-V5

    Plasmid

    #29612

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS-1-413aa (FUS Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Contains FUS aa# 1-413

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

14. 416Gal-FUS-R521G-YFP

    Plasmid

    #29623

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    R521G

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

15. 3I91

    Plasmid

    #62514

    Purpose

    Bacterial expression plasmid for use in structural determination.

    Depositor

    Cheryl Arrowsmith

    Article

    Kaustov et al J Biol Chem. 2011 Jan 7;286(1):521-9. do

    Insert

    CBX8 (CBX8 Human)
    

    Use

    Tags

    6xHis and TEV cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. AFDN_PDZ_1

    Plasmid

    #103938

    Purpose

    Protein expression and purification of TRITHORAX PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    AFDN (AFDN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains AA983-1110 of AFDN

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-shEsr2

    Plasmid

    #120722

    Purpose

    Lentiviral vector that expresses GFP and an shRNA targeting Esr2 (in pLL3.7).

    Depositor

    Amy Lasek

    Article

    Vandegrift et al J Neurosci. 2020 Jul 1;40(27):5196-5207.

    Insert

    Esr2 (Esr2 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA 3.2-FUS-1-269aa-V5

    Plasmid

    #29610

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS-1-269aa (FUS Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Contains FUS aa# 1-269

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pHH0103_LYN-1/1

    Plasmid

    #91536

    Purpose

    Protein expression and purification of human SH3 domain construct LYN-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    LYN-1/1 (LYN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA 3.2-FUS-1-373aa-V5

    Plasmid

    #29611

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS-1-373aa (FUS Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Contains FUS aa# 1-373

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

21. pTE4938

    Plasmid

    #107527

    Purpose

    Expresses Fn crRNA and mCherry in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Fn crRNA
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and human U6

    Available since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPB-TetON-mEGFP-NGN2_WT

    Plasmid

    #215610

    Purpose

    For integration of NGN2 WT T2A mEGFP

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    NGN2 (NEUROG2 Human)
    

    Use

    Tags

    T2A mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LV_EF1a_CDK1-P2A-Hygro_Barcode

    Plasmid

    #170221

    Purpose

    Barcoded lentiviral vector to express CDK1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CDK1 (CDK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 416GPD-FUS-YFP

    Plasmid

    #29594

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 18, 2011

    Availability

    Academic Institutions and Nonprofits only

25. pTE4495

    Plasmid

    #80338

    Purpose

    mammalian expression MbCpf1 nuclease and Mb crRNA

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Mb crRNA
    MbCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and human U6

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDGB3 alpha2 P35S:MS2:EDLL:Tnos (GB1738)

    Plasmid

    #160622

    Purpose

    TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain EDLL

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35s_MS2:EDLL_Tnos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHH0103_ARHGAP32-1/1

    Plasmid

    #91312

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGAP32-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGAP32-1/1 (ARHGAP32 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMG058 MBP-GS 634-666-His

    Plasmid

    #154077

    Purpose

    Expresses MBP-GS 634-666-His (human 33aa Glycogen Synthase peptide as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    GYS1 (GYS1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHH0103_ASAP2-1/1

    Plasmid

    #91504

    Purpose

    Protein expression and purification of human SH3 domain construct ASAP2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ASAP2-1/1 (ASAP2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHH0103_MAP3K9-1/2

    Plasmid

    #91313

    Purpose

    Protein expression and purification of human SH3 domain construct MAP3K9-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    MAP3K9-1/2 (MAP3K9 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge