Skip to main content
Addgene
Showing: 3271 - 3300 of 9706 results

  1. pFA6a-SRM (sortase recognition motif)-hphMX4

    Plasmid
    #54488
    Purpose
    PCR based C-terminal SRM tagging in yeast
    Depositor
    Eri Sakata
    Insert
    SRM (sortase-recognition motif)
    Use
    Yeast genome targeting
    Tags
    hphMX4
    Expression
    Mutation
    Promoter
    Available since
    Aug. 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. pRR1

    Plasmid
    #166096
    Purpose
    Gal inducible expression of Cas9 WT
    Depositor
    Rebecca Burgess
    Insert
    WT spCas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Gal-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166096-plasmid-map-sequence-id-330354

    Enlarge

  3. pPICZa-STE2-1D4

    Plasmid
    #133436
    Purpose
    Mutant form of yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C59S/C252A
    Promoter
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133436-plasmid-map-sequence-id-267728

    Enlarge

  4. pMZ376

    Plasmid
    #74213
    Purpose
    Combined sgRNA/Cas9 vector with KanMX marker
    Depositor
    Mikel Zaratiegui
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    74213-plasmid-map-sequence-id-139302

    Enlarge

  5. pMC6-TEFkFLP(LYS2)

    Plasmid
    #49458
    Purpose
    overespression of Flp step-arrest mutant in yeast, LYS2 selection
    Depositor
    Marc Gartenberg
    Insert
    Flp(H305L) (flp Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    H305L, ‘step-arrest’ mutant,
    Promoter
    TEF1
    Available since
    Dec. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    49458-plasmid-map-sequence-id-73670

    Enlarge

  6. pHCA/GAL4(848).ER

    Plasmid
    #108215
    Purpose
    Yeast expression vector for fusion of Gal4 AA 1-848 to hormone binding domain of estrogen receptor a
    Depositor
    Didier Picard
    Insert
    Gal4-ERalpha HBD
    Use
    Tags
    Expression
    Yeast
    Mutation
    ERalpha HBD has G400V mutation
    Promoter
    ADH0
    Available since
    April 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    108215-plasmid-map-sequence-id-265031

    Enlarge

  7. pFA6a-TRP1-pCUP1-2xphlO

    Plasmid
    #191794
    Purpose
    Yeast gene induction with copper promoter
    Depositor
    Dean Dawson
    Insert
    TRP1 pCUP1 with 2 phlO sites
    Use
    Yeast genomic targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191794-plasmid-map-sequence-id-377878

    Enlarge

  8. pITI2

    Plasmid
    #166095
    Purpose
    Gal inducible expression of D10A-Cas9 nickase
    Depositor
    Rebecca Burgess
    Insert
    Cas9-D10A
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    spCas9-D10A
    Promoter
    Gal-inducible
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    166095-plasmid-map-sequence-id-335585

    Enlarge

  9. pPH3

    Plasmid
    #166098
    Purpose
    Gal inducible expression of D10A-Cas9 nickase
    Depositor
    Rebecca Burgess
    Insert
    Cas9-D10A
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    spCas9-D10A
    Promoter
    Gal-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166098-plasmid-map-sequence-id-330351

    Enlarge

  10. p405ADH1

    Plasmid
    #15966
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    p405ADH1 (ADH1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    ADH1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15966-plasmid-map-sequence-id-8451

    Enlarge

  11. p416-GPD-HA-PPK

    Plasmid
    #183943
    Purpose
    Expresses HA-tagged E. coli PPK from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    PPK (ppk E. coli)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    WT
    Promoter
    GPD
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    183943-plasmid-map-sequence-id-363305

    Enlarge

  12. pRR2

    Plasmid
    #166097
    Purpose
    Gal inducible expression of dCas9-FokI
    Depositor
    Rebecca Burgess
    Insert
    dCas9-FokI
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    spCas9-D10A, H840A
    Promoter
    Gal-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166097-plasmid-map-sequence-id-329109

    Enlarge

  13. pAG416GAL-1x-ccdB-6stop

    Plasmid
    #203519
    Purpose
    Gateway-compatible destination vector for yeast expression with GAL1 promoter (1x GAL4 binding sites)/6stop mutation/URA3 marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    203519-plasmid-map-sequence-id-402864

    Enlarge

  14. pITI1

    Plasmid
    #166094
    Purpose
    Gal inducible expression of dCas9-FokI
    Depositor
    Rebecca Burgess
    Insert
    dCas9-FokI
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    spCas9-D10A, H840A
    Promoter
    Gal-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166094-plasmid-map-sequence-id-329610

    Enlarge

  15. LHP462 - Ub-HA

    Plasmid
    #32169
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    Promoter
    CUP1
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  16. HC080

    Plasmid
    #59545
    Purpose
    Expresses yeast Atg13 under the endogenous promoter with a GFP N-terminal tag
    Depositor
    Daniel Klionsky
    Insert
    ATG13 (ATG13 Budding Yeast)
    Use
    Tags
    GFP
    Expression
    Bacterial and Yeast
    Mutation
    AvrII site created after start codon
    Promoter
    ATG13
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pFA6a-SRM (sortase recognition motif)-kanMX6

    Plasmid
    #54489
    Purpose
    PCR based C-terminal SRM tagging in yeast
    Depositor
    Eri Sakata
    Insert
    SRM (sortase-recognition motif)
    Use
    Yeast genome targeting
    Tags
    kanMX6
    Expression
    Mutation
    Promoter
    Available since
    Aug. 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. LHP905 - Gal1-Ub

    Plasmid
    #32173
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Gal1
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  19. mP_ILB_Vector 1

    Plasmid
    #232193
    Purpose
    The plasmid vector designed for the expression of four genes in oleaginous yeasts, including Yarrowia lipolytica and Rhodotorula toruloides.
    Depositor
    Matrika Bhattarai
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 3, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    232193-plasmid-map-sequence-id-463610

    Enlarge

  20. p416-GPD-DIPP1-HA

    Plasmid
    #183946
    Purpose
    Expresses human HA-tagged DIPP1 from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    DIPP1 (NUDT3 Human)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    WT
    Promoter
    GPD
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183946-plasmid-map-sequence-id-368066

    Enlarge

  21. p416-GPD-DIPP3A-HA

    Plasmid
    #183948
    Purpose
    Expresses human HA-tagged DIPP3A from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    DIPP3A (NUDT10 Human)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    WT
    Promoter
    GPD
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183948-plasmid-map-sequence-id-363374

    Enlarge

  22. p416-GPD-DIPP2-HA

    Plasmid
    #183947
    Purpose
    Expresses human HA-tagged DIPP2 from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    DIPP2 (NUDT4 Human)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    WT
    Promoter
    GPD
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183947-plasmid-map-sequence-id-363373

    Enlarge

  23. p416-GPD-PPK(mutant)

    Plasmid
    #183942
    Purpose
    Expresses catalytic mutant of E. coli PPK from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    PPK(mutant) (ppk E. coli )
    Use
    Tags
    Expression
    Yeast
    Mutation
    mutations in His435, His454, and His592 to Alanine
    Promoter
    GPD
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183942-plasmid-map-sequence-id-363372

    Enlarge

  24. pVS2

    Plasmid
    #33144
    Depositor
    Michael Rosbash
    Article
    Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.
    Inserts
    LacO (256 copies)
    GAL-GFP-kd200-GALpA reporter
    Use
    Tags
    Expression
    Yeast
    Mutation
    GFP is mutated such that amino acids 64-73 (FSYGV…
    Promoter
    bidirectional GAL1-GAL10 promoter
    Available since
    Dec. 20, 2011
    Availability
    Academic Institutions and Nonprofits only

  28. PGAL

    Plasmid
    #60337
    Purpose
    Contains full length native galactose promoter
    Depositor
    Hal Alper
    Article
    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884
    Inserts
    promoter
    yECitrine
    Use
    Synthetic Biology; Yeast synthetic hybrid promote…
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 6, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    60337-plasmid-map-sequence-id-96618

    Enlarge

  30. pTH847-gst-optsc

    Plasmid
    #96908
    Purpose
    Plasmid for yeast expression of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    yeast codon optimised glutathione-S transferase
    Use
    Tags
    8xhistidine
    Expression
    Yeast
    Mutation
    Promoter
    TDH3
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    96908-plasmid-map-sequence-id-206073

    Enlarge

Showing: 3271 - 3300 of 9706 results