We narrowed to 2,813 results for: KIS

1. pCWXPGR-pTF-dnTCF4

   Plasmid

   #114277

   Purpose

   TET-inducible expression of a dominant negative form of the TCF4 transcription factor

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Insert

   TCF4 (TCF4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   deletion of AA 1 to 31

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKM-U6-reRNA-hsHEKsite_1_3

   Plasmid

   #205638

   Purpose

   reRNA guide targeting HEKsite_1_3

   Depositor

   Gerald Schwank

   Article

   Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

   Insert

   reRNA-HEKsite_1_3 (EPHA3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe-Puro-IKBalpha-mut (super repressor)

   Plasmid

   #15291

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   inhibitor of Kappa B alpha (NFKBIA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   S32A/S36A: “super-repressor” allele (resistant to…

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV2.1-CMV-BD lacZ_3' Cer-bGHpA

   Plasmid

   #216319

   Purpose

   Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

   Depositor

   Elvir Becirovic

   Article

   Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

   Insert

   Split Cerulean + splice acceptor site
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV2.1-CMV-5'Cer-BDlacZ-bGHpA

   Plasmid

   #216318

   Purpose

   Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

   Depositor

   Elvir Becirovic

   Article

   Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

   Insert

   split cerulean fluorescent protein + splice donor site
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYUU

   Plasmid

   #159751

   Purpose

   Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

   Plasmid

   #165450

   Purpose

   Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

   Depositor

   Elvir Becirovic

   Article

   Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

   Inserts

   dCas9N-IntN
   3x Opn1mw promoter-targeting sgRNAs
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and short human rhodopsin promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-C2m2-mKate

   Plasmid

   #208793

   Purpose

   For AAV production to express C2m2-mKate in astrocytes

   Depositor

   Urte Neniskyte

   Article

   Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

   Insert

   C2m2-mKate (Mfge8 Mouse)
   

   Use

   AAV

   Tags

   mKate

   Expression

   Mammalian

   Mutation

   Mutated 24 and 45 lysine to asparagine in C2 doma…

   Promoter

   GFAP promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNK2657

   Plasmid

   #219741

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   hispidin- synthase from Mycena citricolor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJFRC7-JEDI-2P

    Plasmid

    #179461

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-2P under the promoter hsp70 for Drosophila (insect) expression

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Insert

    JEDI-2P
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-ALK3 Q233D

    Plasmid

    #80874

    Purpose

    mammalian expression of ALK3 Q233D

    Depositor

    Aristidis Moustakas

    Article

    Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

    Insert

    ALK3 (BMPR1A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q233D (constitutively active)

    Promoter

    CMV

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pNK115

    Plasmid

    #219745

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mutant of fungal luciferase
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    I3S, N4T, F11L, I63T, T99P, T192S, A199P

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNK116

    Plasmid

    #219742

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mutant of fungal hispidin-3-hydroxylase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    D37E, V181I, S323M, M385K

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-C2m2-SNAP

    Plasmid

    #208791

    Purpose

    For AAV production to express C2m2-SNAP in astrocytes

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2m2-SNAP (Mfge8 Mouse)
    

    Use

    AAV

    Tags

    SNAP-tag

    Expression

    Mammalian

    Mutation

    Mutated 24 and 45 lysine to asparagine in C2 doma…

    Promoter

    GFAP promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastbac1-GST-hUSP7

    Plasmid

    #63573

    Purpose

    Expression of synthetic human USP7 tagged with GST

    Depositor

    Titia Sixma

    Article

    Faesen et al Mol Cell. 2011 Oct 7;44(1):147-59. doi:

    Insert

    USP7 (USP7 Synthetic, Human)
    

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Synthetic based on human protein sequence (please…

    Promoter

    Polyhedrin

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-ALK3 K261R

    Plasmid

    #80875

    Purpose

    mammalian expression of ALK3 K261R

    Depositor

    Aristidis Moustakas

    Article

    Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

    Insert

    ALK3 (BMPR1A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K261R (Kinase inactive)

    Promoter

    CMV

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CMV-BD10-SAS620-3’dCas9-VPR-synpA

    Plasmid

    #216323

    Purpose

    Transactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system).

    Depositor

    Elvir Becirovic

    Article

    Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

    Insert

    Split Cas9-VPR + splice acceptor site
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PGL4-4X TE Down (GQs) Renilla

    Plasmid

    #70039

    Purpose

    Plasmid for testing 5’UTR TE Down (G-quadruplex) folding elements

    Depositor

    Hans-Guido Wendel

    Article

    Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

    Insert

    4x TE Down (G-quadruplexes) in 5'UTR
    

    Use

    Luciferase

    Tags

    renilla luciferase

    Expression

    Mammalian

    Mutation

    4 repeats of TE down motif (G-quadruplexes) in 5&…

    Promoter

    SV40

    Available since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pCMV 3xFLAG-ATG3 C264A

    Plasmid

    #129297

    Purpose

    Expresses 3xFLAG-ATG3 C264A in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Cysteine 264 to Alanine

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mGLDC

    Plasmid

    #72881

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

    Insert

    Gldc (Gldc Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only