We narrowed to 22,746 results for: Sele

1. pSAG1::CAS9-U6::sg290860-6

   Plasmid

   #59855

   Purpose

   Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.

   Depositor

   David Sibley

   Article

   Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229

   Insert

   CRISPR sg290860-6
   

   Use

   CRISPR; ; toxoplasma gondii

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQTEV-GYG

   Plasmid

   #31324

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   glycogenin (GYG1 Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-fCR-GFP

   Plasmid

   #22911

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

   Insert

   fugu calretinin promoter driving GFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pRC1765

   Plasmid

   #141346

   Purpose

   Mini plasmid containing minimal sequences for amplification in bacteria and two BbvCI nicking sites for introduction of modified DNA through annealing and ligation (e.g. mismatch, DNA lesions, flap).

   Depositor

   Rafael Fernandez-Leiro

   Article

   Fernandez-Leiro et al Nat Struct Mol Biol. 2021 Apr;28(4):373-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSD117A

   Plasmid

   #218141

   Purpose

   Expresses AviTag-6xHis-MBP-TEV-SD40 for protein purification from E. coli

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   SD40
   

   Use

   Tags

   AviTag-6xHis-MBP-TEV-(GGGGS)2

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-ATG13

   Plasmid

   #171413

   Purpose

   For the purifcation of human ULK1 complex

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   ATG13 (ATG13 Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. JL202: pMVP/mCherry-DEST

   Plasmid

   #121853

   Purpose

   pMVP destination vector, empty expression vector backbone w/ mCherry selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Pmvp gateway destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. JL002: pMVP/eGFP-DEST

   Plasmid

   #121852

   Purpose

   pMVP destination vector, empty expression vector backbone w/ eGFP selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Pmvp gateway destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGATOR

   Plasmid

   #64102

   Purpose

   Tet inducible, Gateway adapted ROSA26 targeting Vector

   Depositor

   Bill Skarnes

   Article

   Melidoni et al Proc Natl Acad Sci U S A. 2013 Oct 29;11

   Insert

   rTTA (Gt(ROSA)26Sor Synthetic)
   

   Use

   Mouse Targeting; Tet inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   ROSA26 endogenous

   Available since

   Dec. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQTEV-PRKRA

    Plasmid

    #31571

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    protein kinase, interferon-inducible double stranded RNA dependent activator (PRKRA Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. Oct4-Cr1

    Plasmid

    #66989

    Purpose

    Expresses gRNA that targets stop codon for OCT4

    Depositor

    Danwei Huangfu

    Article

    Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

    Insert

    gRNA for Crispr targeting the OCT4 stop codon
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pGG184

    Plasmid

    #165604

    Purpose

    Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFP

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    'CAGCG' PAM replacing the 'CGGCG&#…

    Promoter

    lac

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRVGP-BB

    Plasmid

    #15380

    Depositor

    Stephen Smale

    Article

    Ramirez-Carrozzi et al Genes Dev. 2006 Feb 1. 20(3):282-96.

    Insert

    mU6-BRG/BRM shRNA (Smarca4, Smarca2 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pBbdCas9S(-10TC)_Psyn-sgRNA500

    Plasmid

    #149583

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. KN206: pMVP/eCFP-DEST

    Plasmid

    #121854

    Purpose

    pMVP destination vector, empty expression vector backbone w/ eCFP selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Pmvp gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3 Flag Jnk1b2

    Plasmid

    #13752

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk1b2 (MAPK8 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pCMV-Nxph4-HA

    Plasmid

    #128102

    Purpose

    Express HA tagged Nxph4

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nxph4 (Nxph4 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3 Flag Jnk2b2

    Plasmid

    #13757

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk2b2 (MAPK9 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pEBTetD-β-tubulin_SNAP

    Plasmid

    #136832

    Purpose

    Mammalian expression of the centrosomal protein _-tubulin C-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    TUBB_SNAP (TUBB Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHW539 (15xUAS::kin-2a(G310D)::SL2::GFP::let-858 3'UTR)

    Plasmid

    #107137

    Purpose

    Dominant negative PKA cGAL effector for C. elegans

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    kin-2a(G310D)::SL2::GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    15xUAS-delta-pes-10(basal promoter)

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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