We narrowed to 1,112 results for: ENDO 2

1. pNOC_dfnCas12a-KRAB_crRNA Td2

   Plasmid

   #176261

   Purpose

   pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   Kruppel associated box domain and luciferase

   Expression

   Mutation

   D917A and E1006A mutations to inactivate the endo…

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL

   Plasmid

   #73209

   Purpose

   Expresses myc-dL5(E52D)-mCer3 fusion protein in endoplasmic reticulum of mammalian cells with an Igk-leader. (MBIC5, dL5**, FAP)

   Depositor

   Marcel Bruchez

   Article

   Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

   Insert

   kappa-myc-dL5-2XG4S-mCer3-KDEL (MYC Synthetic, Human)
   

   Use

   Tags

   The FAP and mCerulean3 are fused with 2 copies of…

   Expression

   Mammalian

   Mutation

   The dL5** FAP is E50D, L89S, also known as E52D/L…

   Promoter

   CMV

   Available since

   March 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-YAP-mEGFP

   Plasmid

   #166472

   Purpose

   HDR template for tagging of endogenous mouse YAP N-terminus with mEGFP

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   YAP-mEGFP HDR template(mouse Yap) (Yap1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFUSE-rIgG-Fc2-ICAM1

   Plasmid

   #156462

   Purpose

   Expresses a rabbit Fc-ICAM1 fusion protein in mammalian cells

   Depositor

   Dirk Mielenz

   Article

   Reimer et al Cell Rep. 2020 Aug 11;32(6):108030. doi:

   Insert

   Intercellular Adhesion Molecule 1 (Icam1 Mouse)
   

   Use

   Tags

   ICAM1-rFc

   Expression

   Bacterial and Mammalian

   Mutation

   endogenous signal peptide replaced with IL2 signa…

   Promoter

   hEF1-HTLC

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

   Plasmid

   #196084

   Purpose

   (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Inserts

   dCas9-KRAB-MeCP2 repressor
   hU6 promoter and gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Catalytically dead mutant of the Cas9 endonucleas…

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI

   Plasmid

   #196078

   Purpose

   (Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Inserts

   dCas9-KRAB-MeCP2 repressor
   hU6 promoter and gRNA scaffold
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Catalytically dead mutant of the Cas9 endonucleas…

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

   Plasmid

   #196076

   Purpose

   (Empty Backbone) Constitutive CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Inserts

   dCas9-KRAB-MeCP2 repressor
   hU6 promoter and gRNA scaffold
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Catalytically dead mutant of the Cas9 endonucleas…

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI

   Plasmid

   #196074

   Purpose

   (Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Inserts

   dCas9-KRAB-MeCP2 repressor
   hU6 promoter and gRNA scaffold
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Catalytically dead mutant of the Cas9 endonucleas…

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYX-N-Puro-mNeon-TSC22D1

   Plasmid

   #222667

   Purpose

   endogenously tag TSC22D1 with N-terminal mNeon

   Depositor

   Jason Moffat

   Article

   Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

   Insert

   TSC22D1 (TSC22D1 Human)
   

   Use

   Tags

   mNeon

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196082

    Purpose

    (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Aminoglycoside phosphotransferase
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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