We narrowed to 10,458 results for: T7

1. pJK268

   Plasmid

   #72436

   Purpose

   Produces Acetobacter aceti 1023 del7-GloA

   Depositor

   T. J. Kappock

   Insert

   lactoylglutathione lyase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Missing residues 1-7

   Promoter

   T7

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK627

   Plasmid

   #72462

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asp/Tyr154>Phe mutant (AaPurE1-S57D,Y154F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mutation changes serine-57 to aspartate, tyrosine…

   Promoter

   T7

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJK628

   Plasmid

   #72463

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Ala/Tyr154>Phe mutant (AaPurE1-S57A,Y154F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mutation changes serine-57 to alanine, tyrosine-1…

   Promoter

   T7

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK573

   Plasmid

   #72456

   Purpose

   Produces Yersinia pestis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tYpPurE1), synthetic gene

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   synthetic gene with ~57 silent substitutions

   Promoter

   T7

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK632

   Plasmid

   #72465

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Val/Tyr154>Phe mutant (AaPurE1-S57V,Y154F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mutation changes serine-57 to valine, tyrosine-15…

   Promoter

   T7

   Available since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJK629

   Plasmid

   #72464

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Thr/Tyr154>Phe mutant (AaPurE1-S57T,Y154F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mutation changes serine-57 to threonine, tyrosine…

   Promoter

   T7

   Available since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK640

   Plasmid

   #72467

   Purpose

   Encodes Escherichia coli evgA

   Depositor

   T. J. Kappock

   Insert

   response regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJK541

   Plasmid

   #72447

   Purpose

   Produces Acetobacter aceti 1023 small hypothetical protein (AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)

   Depositor

   T. J. Kappock

   Inserts

   hypothetical protein
   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 and n/a

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK543

   Plasmid

   #72516

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); large subunit (AaPurL)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   contains additional Ala2

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK587

    Plasmid

    #71696

    Purpose

    Produces Acetobacter aceti 1023 thioredoxin reductase 1 with a C-terminal His6 tag and Cys138>Ser mutant (AaTrxB1H6-C138S)

    Depositor

    T. J. Kappock

    Insert

    thioredoxin reductase 1
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes cysteine-138 to serine

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK601

    Plasmid

    #71606

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Tyr154>Phe mutant (AaPurE1-Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes tyrosine-154 to phenylalanine, additional…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 4Z31

    Plasmid

    #69958

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RC3H2:YTC020-E10:C234061 (RC3H2 Human)
    

    Use

    Tags

    His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO

    Available since

    Oct. 7, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. 4Z0O

    Plasmid

    #69954

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ZCWPW2:JMC01B-H12:C203050 (ZCWPW2 Human)
    

    Use

    Tags

    His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO

    Available since

    Oct. 7, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. 4QQM

    Plasmid

    #61950

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RFXANK:JMC029-A03:C213522 (RFXANK Human)
    

    Use

    Tags

    6xHis and MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

15. 2I7C

    Plasmid

    #60124

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PY03671:MAC01H-E01:C21952
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available since

    Nov. 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

16. 4IIM

    Plasmid

    #51325

    Depositor

    Cheryl Arrowsmith

    Insert

    4IIM (ITSN1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available since

    Feb. 24, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. 4IIO

    Plasmid

    #51326

    Depositor

    Cheryl Arrowsmith

    Insert

    4IIO (ITSN2 4IIO, Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available since

    Feb. 24, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. hKCNQ5(WT).ires.GFP-pcDNA3

    Plasmid

    #204360

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7 RNApol

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. hKCNQ3(WT).ires.mCherry-pcDNA3

    Plasmid

    #204362

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ3 (KCNQ3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7 RNApol

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hBACE1(D289N).ires.mCherry-pcDNA3

    Plasmid

    #204363

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    BACE1 (BACE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D289N

    Promoter

    CMV/T7 RNApol

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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