We narrowed to 4,947 results for: mcs

1. pET21-pelB-LaG-12

   Plasmid

   #172744

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-12; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-12
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaG-14

   Plasmid

   #172745

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-14; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-14
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJL1-neoR

   Plasmid

   #117048

   Purpose

   In vitro expression of neoR (kanamycin resistance gene) from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

   Insert

   neoR
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSP64-hERG1a D540K

   Plasmid

   #53057

   Purpose

   Expresses alpha subunit of D540K hERG1a K channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Sanguinetti et al J Physiol. 1999 Feb 1;514 ( Pt 3):667-75

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Xenopus oocyte expression

   Mutation

   changed Aspartate 540 to Lysine

   Promoter

   SP6

   Available Since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSP64-hERG1a S620T

   Plasmid

   #53059

   Purpose

   Expresses alpha subunit of S620T hERG1a K channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Casis et al Mol Pharmacol. 2006 Feb;69(2):658-65. Ep

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Xenopus oocyte expression

   Mutation

   changed Serine 620 to Threonine

   Promoter

   SP6

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSP64-hERG1a S631A

   Plasmid

   #53058

   Purpose

   Expresses alpha subunit of S631A hERG1a K channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Zou et al J Physiol. 1998 May 15;509 ( Pt 1):129-3

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Xenopus oocyte expression

   Mutation

   changed Serine 631 to Alanine

   Promoter

   SP6

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDLucBiDi

   Plasmid

   #219562

   Purpose

   Dual luciferase plasmid with Renilla luciferase constitutively expressed and firefly luciferase downstream of MCS without a start codon

   Depositor

   Pasha Baranov

   Type

   Empty backbone

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   SV40 and T7

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTol2-nbt300 2n-KalNFB-3xmir1T-syt2a

   Plasmid

   #196900

   Purpose

   zebrafish expression

   Depositor

   Reinhard Köster

   Insert

   nbt300 2n-KalNFB-3xmir1T-syt2a
   

   Use

   Zebrafish expression

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAM-PAT-ProATE2 GW

   Plasmid

   #80682

   Purpose

   binary DEST vector for plant expression

   Depositor

   Nico Dissmeyer

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   ProATE2

   Available Since

   Aug. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAM-PAT-ProATE1 GW

    Plasmid

    #80681

    Purpose

    binary DEST vector for plant expression

    Depositor

    Nico Dissmeyer

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    ProATE1

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH-EF1-copGFP

    Plasmid

    #73030

    Purpose

    Express copGFP

    Depositor

    Kazuhiro Oka

    Insert

    copGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDH-CB-iCre

    Plasmid

    #72257

    Purpose

    Express iCre under CB promoter

    Depositor

    Kazuhiro Oka

    Insert

    iCre
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CB promoter and chicken beta-actin + CMV enhancer

    Available Since

    Jan. 15, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pCDH-EF1-Fon-copGFP

    Plasmid

    #72260

    Purpose

    Express copGFP when FLP is expressed by another vector

    Depositor

    Kazuhiro Oka

    Insert

    copGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    elongation factor-1

    Available Since

    Jan. 11, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP

    Plasmid

    #176027

    Purpose

    construct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometry

    Depositor

    Irmela Jeremias

    Article

    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

    Inserts

    rtTA3
    Luc2
    mtagBFP
    

    Use

    Lentiviral

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 2xFLAG-2xSTREP E2F4

    Plasmid

    #236439

    Purpose

    transient overexpression of E2F4 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F4 (E2F4 Human)
    

    Tags

    FLAG-FLAG-STREP-STREP

    Expression

    Mammalian

    Available Since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. HER2 WT

    Plasmid

    #16257

    Depositor

    Mien-Chie Hung

    Article

    Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

    Insert

    HER2 (ERBB2 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJL1

    Plasmid

    #69496

    Purpose

    pJL1 expression vector with sfGFP

    Depositor

    Michael Jewett

    Article

    Martin et al Nat Commun. 2018 Mar 23;9(1):1203. doi:

    Insert

    Super folder GFP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. scFv-sfGFP-DNMT3A1

    Plasmid

    #102278

    Purpose

    The plasmid encodes a single-chain antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a DNA methyltransferase DNMT3A1

    Depositor

    Margaret Goodell

    Article

    Huang et al Genome Biol. 2017 Sep 18;18(1):176. doi:

    Insert

    DNMT3A1 (DNMT3A Human)
    

    Use

    Lentiviral

    Tags

    scFV-sfGFP

    Expression

    Mammalian

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3.1 hSCN5A

    Plasmid

    #145374

    Purpose

    expresses human Nav1.5 (WT) in mammalian cells

    Depositor

    Robert Kass, Hugues Abriel

    Article

    Rougier et al Am J Physiol Cell Physiol. 2005 Mar;288(

    Insert

    sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV-CASANOVA

    Plasmid

    #113035

    Purpose

    CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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