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HA-MBP-TEVpCS-ADGRD1 CTFdeltaTA-FLAG

Plasmid

#217720

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRD1 (ADGRD1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-550

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG3 CTF-FLAG

Plasmid

#217701

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRG3 (ADGRG3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-249

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRF1 CTFdeltaTA-FLAG

Plasmid

#217727

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRF1 (ADGRF1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-572

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG4 CTFdeltaTA-FLAG

Plasmid

#217735

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRG4 (ADGRG4 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2727

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG3 CTFdeltaTA-FLAG

Plasmid

#217734

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRG3 (ADGRG3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-255

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG7 CTFdeltaTA-FLAG

Plasmid

#217738

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRG7 (ADGRG7 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-421

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)

Plasmid

#229828

Purpose

A Piggybac-compatible, cre-dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pGM32DEST

Plasmid

#74747

Purpose

Encodes for the QF-GR recombinant gene, splice linker, and mCherry reporter gene

Depositor

Jordan Ward, Keith Yamamoto

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Inserts

Gateway cassette
QF-GR (NR3C1 Human, Synthetic, Neurospora crassa)

Tags

Fusion of the Neurospora crassa QF activator to t…

Expression

Worm

Promoter

No promoter

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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FKBP-LBR(1-245)-GFP

Plasmid

#222425

Purpose

To express a truncated LBR (encoding amino acids 1-245) with N-terminal FKBP tag accessible to the cytoplasm and C-terminal GFP in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

LBR(1-245aa) (LBR Human)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Truncation (Includes aa 1-245)

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarG

Plasmid

#31221

Depositor

Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Insert

CD4-tdGFP (CD4 Human, Fly, Aequorea victoria)

Tags

CD4 and EGFP/GFP

Expression

Insect

Mutation

Fusion of signal peptide of Drosophila Akh gene, …

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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