We narrowed to 5,295 results for: pcdna3.1

1. pcDNA3.1-GSDMD_29-270

   Plasmid

   #218232

   Purpose

   Gasdermin-D_29-270 is a non-functional GSDMD formed by two SARS-CoV-2 3CLpro cleavages. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   GSDMD (GSDMD Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   construct only codes for amino acids 29-270

   Promoter

   Available since

   July 1, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pcDNA3.1-HaloAKAR2.1-CLC

   Plasmid

   #216492

   Purpose

   Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) targeted to clathrin coated pits

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloAKAR2.1-CLC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-flag-IARS2-Δ1-48

   Plasmid

   #232341

   Purpose

   Expresses human IARS2 with mito-location signal replaced by flag tag in mammalian cells, also carrying synonymous mutations described in Addgene #232340

   Depositor

   Quanfu Ma

   Insert

   IARS2 (IARS2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-48 (indicated as Mito-signal in UniPro…

   Promoter

   CMV

   Available since

   June 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1(+)-FLAG-Rgs14

   Plasmid

   #236396

   Purpose

   Expresses FLAG-Rgs14 in mammalian cells

   Depositor

   John Hepler

   Article

   Shu et al Cell Signal. 2007 Jan;19(1):163-76. doi:

   Insert

   Rattus norvegicus regulator of G-protein signaling 14 (Rgs14 Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1+ dgat1b-FLAG

   Plasmid

   #232144

   Purpose

   Expresses zebrafish dgat1b with a C-terminal FLAG tag under control of the CMV promoter

   Depositor

   Steven Farber

   Article

   Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

   Insert

   dgat1b
   

   Use

   Zebrafish expression

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-hSyncytin-1(AAAR)

   Plasmid

   #182442

   Purpose

   hSyncytin-1, furin cleavage site RNKR>AAAR(314-317)

   Depositor

   Masaharu Somiya

   Insert

   ERVW-1 (ERVW-1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   furin cleacage site RNKR>>AAAR(314-317)

   Promoter

   CMV

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-hSyncytin-1(deltaFP)

   Plasmid

   #182443

   Purpose

   hSyncytin-1, fusion peptide deletion(320-340)

   Depositor

   Masaharu Somiya

   Insert

   ERVW-1 (ERVW-1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   no fusion peptide (320-340)

   Promoter

   CMV-F

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-hSyncytin-1(C186A)

   Plasmid

   #182450

   Purpose

   hSyncytin-1, fusion deficit and dominant negative mutant (C186A)

   Depositor

   Masaharu Somiya

   Insert

   ERVW-1 (ERVW-1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C186A

   Promoter

   CMV

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1 Flag-TUBG2

   Plasmid

   #229855

   Purpose

   TUBG2

   Depositor

   Maria Alvarado-Kristensson

   Article

   Lindstrom et al FASEB J. 2025 Mar 15;39(5):e70431. doi:

   Insert

   TUBG2 (TUBG2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Removal of the internal TUBG2-EcoRI site by chang…

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 N-T2A-M-IRES-E

    Plasmid

    #231903

    Purpose

    For the production of SARS-CoV-2 virus-like particles (VLPs) in the '3-plasmid' system

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    nucleocapsid protein-T2A-membrane protein-IRES-envelope protein (N, M, E SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R203M in N protein

    Promoter

    CMV

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge