We narrowed to 3,796 results for: golden gate

1. sgPPP1R2

   Plasmid

   #227391

   Purpose

   Adhesion module, guide RNA recognizes PPP1R2

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   guide RNA targeting PPP1R2
   

   Use

   Lentiviral

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNP1950

   Plasmid

   #222729

   Purpose

   J23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 Recombinase

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PAC_E83_LOV_eGFP

   Plasmid

   #231574

   Purpose

   This plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue light

   Depositor

   Dominik Niopek

   Article

   Wolf et al bioRxiv 2024.12.04.626757

   Insert

   PAC-P2A-GFP carrying an insertion of AsLOV2 behind E83
   

   Expression

   Mammalian

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ132B

   Plasmid

   #69282

   Purpose

   Golden Gate entry vector; 2nd gRNA under AtU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUS-mCh-sspB

   Plasmid

   #227377

   Purpose

   Condensate module, light-inducible oligomerization IDR

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   FUS IDR
   

   Use

   Lentiviral

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. NLS-GFP-iLID-Ferritin

   Plasmid

   #227376

   Purpose

   Condensate module, light-inducible oligomerization 'core'

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   GFP 'core'
   

   Use

   Lentiviral

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-BbsI-PITCh

   Plasmid

   #127875

   Purpose

   For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-UPEmS-tevo

   Plasmid

   #228894

   Purpose

   Golden gate cloning (BsmBI) of pegRNAs with tevopreQ1 motif, and with constitutive RFP expression.

   Depositor

   Francisco J Sánchez-Rivera

   Article

   Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEM790

   Plasmid

   #242607

   Purpose

   T-DNA plasmid, Level 2 destination vector, replaces pMIN-VS1

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Sept. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tad-CBE(ACC2)

    Plasmid

    #235061

    Purpose

    Expresses Tad-CBE(ACC2) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(ACC2)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, SV40NLS and bpNLS

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tad-CBE(ACC3)

    Plasmid

    #235062

    Purpose

    Expresses Tad-CBE(ACC3) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(ACC3)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, SV40NLS and bpNLS

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKG1334_pHarbor-PiggyBac_backbone

    Plasmid

    #239705

    Purpose

    PaqCI Golden Gate assembly plasmid encoding the backbone for PiggyBac-mediated integration

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEE082 FoMV

    Plasmid

    #234811

    Purpose

    To clone guide RNA (gRNA) along with its scaffold in to the Foxtail Mosaic Virus Vector (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPD304 VPR-ZF43

    Plasmid

    #138840

    Purpose

    Constitutive expression of VPR-ZF43 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VPR-ZF43
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tad-CBE(TCA3)

    Plasmid

    #235064

    Purpose

    Expresses Tad-CBE(TCA3) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(TCA3)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICH-nuc-roGFP2-ORP1

    Plasmid

    #191758

    Purpose

    Ti plasmid pICH86988 harbouring nuc-roGFP2-ORP1 for peroxide detection in the nuclei of plant cells.

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    nuc-roGFP2-ORP1
    

    Use

    Binary agrobacterium ti plasmid

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJPC596 FoMV-AmCyan

    Plasmid

    #234812

    Purpose

    To express flourescent protein AmCyan from the Foxtail Mosaic Virus Vector (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Insert

    AmCyan
    

    Expression

    Plant

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCHW91015

    Plasmid

    #222455

    Purpose

    Level 1, Position 2 containing Mas::CUP2-VP16::NOS.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    Mas::CUP2-VP16::NOS (CUP2 )
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    no

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDV002

    Plasmid

    #191823

    Purpose

    Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the NS1 locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: aadA; E. coli: AmpR

    Depositor

    Jim Roberts

    Article

    Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGG-GOI-DE-TurboID-GFP

    Plasmid

    #209389

    Purpose

    Golden Gate compatible plasmid for TurboID-based proximity labelling experiment in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Type

    Empty backbone

    Tags

    GFP

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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