Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 6,923 results for: pcdna3.1

Showing: 2221 - 2240 of 6923 results

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pcDNA3.1-SARS-CoV-2-S (D614G)

Plasmid

#215838

Purpose

Expresses SARS-CoV-2 Spike protein in mammalian cells

Depositor

Thomas Gallagher

Publication

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Spike (S )

Expression

Mammalian

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-KB2123-Nluc

Plasmid

#204325

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Publication

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

KB2123-Nluc

Expression

Mammalian

Available since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1(+)Gαq-124-NLuc

Plasmid

#207153

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Publication

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAQ (GNAQ Human)

Expression

Mammalian

Mutation

NLuc inserted at position F(124)E

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)GαsS-246-Nluc

Plasmid

#207157

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes short transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Publication

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAS (GNAS Human)

Expression

Mammalian

Mutation

NLuc inserted at position D(246)N

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)GαsL-260-NLuc

Plasmid

#207158

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Publication

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAS (GNAS Human)

Expression

Mammalian

Mutation

NLuc inserted at position D(260)N

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gγ1-LSS-mKATE2(N)

Plasmid

#207159

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG1 tagged with LSS-mKATE2 to be paired with one of the GNA

Depositor

Dominique Schols, Tom Van Loy

Publication

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNG1

Expression

Mammalian

Mutation

N-terminally tagged with LSS-mKATE2

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gγ2-LSS-mKATE2(N)

Plasmid

#207160

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNA

Depositor

Dominique Schols, Tom Van Loy

Publication

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNG2 (GNG2 Human)

Expression

Mammalian

Mutation

N-terminally tagged with LSS-mKATE2

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-RasAR(R89L)-NES

Plasmid

#205568

Purpose

RasAR negative-control mutant plasmid. Targeted to cytosol.

Depositor

Jin Zhang

Publication

Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

Insert

RasAR(R89L)-NES (RAF1 Synthetic, Human)

Tags

Nuclear export signal (NES)

Expression

Mammalian

Mutation

Arg 89 mutated to Leu in Raf1 RBD region.

Promoter

CMV

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP Rab11a WT pcDNA3.1 Zeo (+)

Plasmid

#206079

Purpose

Rab11a belongs to the Rab family of small GTPases, Rab11 proteins are particularly involved in trafficking through recycling endosomes

Depositor

Annette Dolphin

Publication

Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

Insert

RAB11A (RAB11A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-Hs4E-T_AA

Plasmid

#148233

Purpose

Mammalian Expression of Hs4E-T

Depositor

Elisa Izaurralde

Insert

Hs4E-T (EIF4ENIF1 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) HuTF-Fc-His tag

Plasmid

#183253

Purpose

Soluble expression of the extracellular domains of human tissue factor (TF)-Fc

Depositor

Alexander McLellan

Insert

Tissue factor (F3 Human)

Tags

8xHis

Expression

Mammalian

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRA-30-picky_sticky

Plasmid

#138983

Purpose

IVT template for the alpha subunit of the mouse TCR #30 that is reactive against MC38

Depositor

Jeff Hammerbacher

Publication

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRB-33-MC_hammer

Plasmid

#138990

Purpose

IVT template for the beta subunit of the mouse TCR #33 that is reactive against MC38

Depositor

Jeff Hammerbacher

Publication

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRA-33-MC_hammer

Plasmid

#138989

Purpose

IVT template for the alpha subunit of the mouse TCR #33 that is reactive against MC38

Depositor

Jeff Hammerbacher

Publication

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRB-32-riot_punisher

Plasmid

#138988

Purpose

IVT template for the beta subunit of the mouse TCR #32 that is reactive against MC38

Depositor

Jeff Hammerbacher

Publication

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRA-32-riot_punisher

Plasmid

#138987

Purpose

IVT template for the alpha subunit of the mouse TCR #32 that is reactive against MC38

Depositor

Jeff Hammerbacher

Publication

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-MC38-TCRB-31-murderous_crow

Plasmid

#138986

Purpose

IVT template for the beta subunit of the mouse TCR #31 that is reactive against MC38

Depositor

Jeff Hammerbacher

Publication

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-MC38-TCRA-31-murderous_crow

Plasmid

#138985

Purpose

IVT template for the alpha subunit of the mouse TCR #31 that is reactive against MC38

Depositor

Jeff Hammerbacher

Publication

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-MC38-TCRB-30-picky_sticky

Plasmid

#138984

Purpose

IVT template for the beta subunit of the mouse TCR #30 that is reactive against MC38

Depositor

Jeff Hammerbacher

Publication

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS-mFndc3b

Plasmid

#126443

Purpose

Expresses mouse Fndc3b circular RNA

Depositor

Jeremy Wilusz

Publication

Garikipati et al Nat Commun. 2019 Sep 20;10(1):4317. doi:

Insert

Fndc3b (Fndc3b Mouse)

Expression

Mammalian

Mutation

circFndc3b exons are separated by a miniature int…

Promoter

CMV

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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Showing: 2221 - 2240 of 6923 results

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