We narrowed to 10,632 results for: t7

1. pT7CFE1_EVD68_3ABC

   Plasmid

   #212979

   Purpose

   ncodes EV-D68 MO/14/18947 3ABC optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 3ABC
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Promoter

   T7

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7CFE1_EVD68_3ABCD

   Plasmid

   #212981

   Purpose

   Encodes EV-D68 MO/14/18947 3ABCD optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 3ABCD
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Promoter

   T7

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEMHE-TNKS-2-M1054V

   Plasmid

   #138538

   Purpose

   For making TNKS-2-M1054V RNA

   Depositor

   Carmen Williams

   Insert

   Tankyrase 2 (TNKS2 Human)
   

   Use

   In vitro transcription

   Tags

   FLAG

   Mutation

   Changed methionine 1054 to valine

   Promoter

   T7

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSP1D12W/2Y

   Plasmid

   #124275

   Purpose

   Study of protein insertion into nanodisks by means of changes in tryptophan fluorescence

   Depositor

   Alexey S. Ladokhin

   Insert

   Membrane scaffold protein
   

   Tags

   7xHis-tag

   Expression

   Bacterial

   Mutation

   Two tryptophans changed to two tyrosines

   Promoter

   T7

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmCellFree_KA1_SARS-COV-2_N

   Plasmid

   #169401

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus N with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _N (N SARS-Cov2)
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Promoter

   T7- CMV/CAG

   Available Since

   July 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmCellFree_KA1_SARS-COV-2_ORF6

   Plasmid

   #169392

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus ORF6 with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _ORF6 (ORF6 SARS-Cov2)
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Promoter

   T7- CMV/CAG

   Available Since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. 6WAT

   Plasmid

   #162268

   Purpose

   Baculovirus expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   PHF1 (PHF1 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. 6PED

   Plasmid

   #162265

   Purpose

   Baculovirus expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   HEMK2 (N6AMT1 Human)
   

   Tags

   6xHis-Thrombin

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. TBXTA-c025

   Plasmid

   #139762

   Purpose

   Protein expression/GST pull-down

   Depositor

   Opher Gileadi

   Insert

   TBXT, Full-length, G177D (TBXT Human)
   

   Tags

   His6-GST-TEV

   Expression

   Bacterial

   Mutation

   G177D; contains only amino acids S2- M435

   Promoter

   T7

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. TBXTA-c024

    Plasmid

    #139761

    Purpose

    Protein expression/GST pull-down

    Depositor

    Opher Gileadi

    Insert

    TBXT, DNA-binding domain, G177D (TBXT Human)
    

    Tags

    His6-GST-TEV

    Expression

    Bacterial

    Mutation

    G177D; contains only amino acids E41-D225

    Promoter

    T7

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. TBXTA-c023

    Plasmid

    #139760

    Purpose

    Protein expression/GST pull-down

    Depositor

    Opher Gileadi

    Insert

    TBXT, DNA-binding domain, WT (TBXT Human)
    

    Tags

    His6-GST-TEV

    Expression

    Bacterial

    Mutation

    Contains only amino acids E41-D225

    Promoter

    T7

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. 6CW0 (cgd2_2690)

    Plasmid

    #119965

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    cgd2_2690
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    contains amino acids 1-105

    Promoter

    T7

    Available Since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. STK10 (STK10A-c043)

    Plasmid

    #110255

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    STK10 (STK10 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7 - lacO

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7-7 asyn S129W

    Plasmid

    #105744

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein S129W (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    S129W

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-7 asyn K32R

    Plasmid

    #105739

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein K32R (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    K32R

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET11a asyn Q79N (TAC-->TAT)

    Plasmid

    #105733

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein Q79N (TAC-->TAT) (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    Q79N

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. 5J3U (TgPKA, 583.m05422)

    Plasmid

    #79525

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    583.m05422
    

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO

    Available Since

    June 15, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. 5FEA (LdBRD19, LdBPK_363520.1)

    Plasmid

    #79520

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    LdBPK_363520.1
    

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO

    Available Since

    June 15, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. 5CZG (Tb427.10.7420)

    Plasmid

    #79517

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    Tb427.10.7420
    

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO

    Available Since

    June 15, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pJK626

    Plasmid

    #72461

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Cys/Tyr154>Phe mutant (AaPurE1-S57C,Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    Mutation changes serine-57 to cysteine, tyrosine-…

    Promoter

    T7

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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