We narrowed to 31,888 results for: Eng

1. pET21a(+)_co_L-HicDH

   Plasmid

   #185453

   Purpose

   CDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).

   Depositor

   Wolfgang Kroutil

   Article

   Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

   Insert

   L-HicDH from Lactobacillus confusus DSM 20196
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEVA251_ Ptrc.x.tetO2::D-HicDH

   Plasmid

   #185456

   Purpose

   CDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.

   Depositor

   Wolfgang Kroutil

   Article

   Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

   Insert

   D-HicDH from Lactobacillus paracasei DSM 20008
   

   Expression

   Bacterial

   Promoter

   Ptrc.x.tetO2

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTS2329

   Plasmid

   #169629

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (PhCMV-SEAP-p2A-iRFP670-pA::spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and spacer-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Expression

   Mammalian

   Promoter

   PhCMV / PPGK

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS2322

   Plasmid

   #169627

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and Tactb-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Expression

   Mammalian

   Promoter

   PhCMV / PPGK

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTS2320

   Plasmid

   #169625

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and spacer-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Expression

   Mammalian

   Promoter

   PhCMV / PPGK

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTS2312

   Plasmid

   #169622

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Expression

   Mammalian

   Promoter

   PhCMV / PPGK

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTS2317

   Plasmid

   #169624

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Expression

   Mammalian

   Promoter

   PhCMV / PPGK

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPPC002

   Plasmid

   #171139

   Purpose

   For integration of J1-J23117-sfGFP, Sp.pCas9-dCas9, and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   J1-BBa_J23117-sfGFP
   Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   SoxS has R93A and S101A mutations

   Promoter

   J1-BBa_J23117 and Sp.pCas9, BBa_J23107

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKdH_P-GAP-ScACS1*

   Plasmid

   #126736

   Purpose

   yeast plasmid overexpressing ACS1 from Saccharomyces cerevisiae

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   acs1*
   

   Tags

   6xHis,Myc

   Expression

   Yeast

   Mutation

   T3542C and A30S, P113S, and S629N (please see dep…

   Promoter

   GAP

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKdH_P-GAP-ScADH2+P-GAP*-ScACS1*

    Plasmid

    #126738

    Purpose

    yeast plasmid overexpressing ADH2 and ACS1 from Saccharomyces cerevisiae

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    adh2,acs1*
    

    Tags

    6xHis,Myc

    Expression

    Yeast

    Mutation

    T3542C

    Promoter

    GAP

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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