We narrowed to 5,443 results for: SPI

1. pME-LexA-mODC (no LexOp) (JDW 937)

   Plasmid

   #224504

   Purpose

   Gateway compatible middle entry clone containing a destabilized LexA transactivator

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   LexA-mODC
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME-myc-BirA (JDW 1210)

   Plasmid

   #224509

   Purpose

   Gateway compatible middle entry clone containing a Myc tagged BirA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Myc-BirA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-Myc-BioID2-MCS1 (JDW 1095)

   Plasmid

   #224510

   Purpose

   Gateway compatible middle entry clone containing a Myc-tagged BIOID2 construct

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Myc-BioID2
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3E-H2A_mCherry_SV40pA (JDW 967)

   Plasmid

   #224528

   Purpose

   A Gateway compatible 3' entry clone containing an H2A mCherry fusion followed by SV40 late polyA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   H2A-mCherry-SV40pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3E-V5-mClover3-pA (JDW 1318)

   Plasmid

   #224530

   Purpose

   A Gateway compatible 3' entry clone containing a V5 tagged mClover3 followed by a polyA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   V5-mClover3-pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3E-IRES-mTagBFP2-pA (JDW 1361)

   Plasmid

   #224535

   Purpose

   A Gateway compatible 3' entry clone containing an IRES-FLAG-NLS-mTagBFP2-pA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   IRES-FLAG-NLS-mTagBFP2-pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3E-IRES-(n2)StayGold(c4)-pA (JDW 1364)

   Plasmid

   #224537

   Purpose

   A Gateway compatible 3' entry clone containing IRES-(n2)StayGold(c4)-pA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   IRES-(n2)StayGold(c4)-pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221-Lifeact- mScarlet-HA (JDW 1247)

   Plasmid

   #224538

   Purpose

   A Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Lifeact-mScarlet-HA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME H2B mCerulean (JDW 1150)

   Plasmid

   #224543

   Purpose

   A Gateway compatible middle entry clone containing a histone H2B fusion to mCerulean to label the nucleus

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   H2B-mCerulean
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPRISM-QF2-he1.1:YFP

    Plasmid

    #184819

    Purpose

    Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    QF2-pA-he1.1:YFP
    

    Use

    CRISPR; Backbone for cloning of homology arms (fo…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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