We narrowed to 10,492 results for: nar

1. pCH6 - pBait-ChiX

   Plasmid

   #174662

   Purpose

   arabinose-induced synthesis of a hybrid RNA containing a single MS2 hairpin followed by E coli ChiX inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.

   Depositor

   Katherine Berry

   Article

   Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507

   Insert

   ChiX
   

   Use

   Tags

   1xMS2 hairpin

   Expression

   Bacterial

   Mutation

   Promoter

   pBad

   Available since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. HB401: pMVP (L1-L4) CMV promoter

   Plasmid

   #121686

   Purpose

   pMVP L1-L4 entry plasmid, contains CMV promoter for 3-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   CMV promoter
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28b-His-hCE

   Plasmid

   #112710

   Purpose

   Expresses N-terminally His-tagged human CE/RNGTT in bacterial cells

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   RNGTT (RNGTT Human)
   

   Use

   Tags

   6xHis and Stop codons placed after RNGTT sequence…

   Expression

   Bacterial

   Mutation

   R594H

   Promoter

   T7

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. SL2-sgTelo-MTSa/BFP/pdCas9-C1

   Plasmid

   #162760

   Purpose

   Expressing dCas9 and sgRNA containg MTSa targeting telomeres

   Depositor

   Antony K Chen

   Article

   Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

   Insert

   dCas9 and sgRNA(SL2-sgTelo-MTSa)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dCas9(nuclease deactivated Cas9)

   Promoter

   U6/CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPO685

   Plasmid

   #195300

   Purpose

   pBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.

   Depositor

   Joseph Peters

   Article

   Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

   Insert

   McCAST Cascade and native array with PaqCI entry site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Arabinose inducible arapBAD

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AV44_pCAG.Cas9-D10A.gRNA.S1

   Plasmid

   #199258

   Purpose

   Expression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNA

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Inserts

   SpCas9-D10A nickase
   AAVS1-targeting gRNA
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG promoter and RNA polymerase III promoter for …

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUD628

   Plasmid

   #103018

   Purpose

   expression of a Cpf1 programming crRNA targetting ADE2 (crADE2-3.S)

   Depositor

   Jean-Marc Daran

   Article

   Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

   Insert

   crADE2-3.S (ADE2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. SL2-sgTelo-MTSa/EGFP/pdCas9-C1

   Plasmid

   #162759

   Purpose

   Expressing dCas9 and sgRNA containg MTSa targeting telomeres

   Depositor

   Antony K Chen

   Article

   Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

   Insert

   dCas9 and sgRNA(SL2-sgTelo-MTSa)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dCas9(nuclease deactivated Cas9)

   Promoter

   U6/CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-hCas9-T2A-miRFP670

   Plasmid

   #187223

   Purpose

   Expresses human Cas9 nuclease and miRFP670 via T2A linker under control of a CMV promoter.

   Depositor

   Jennifer Mitchell

   Article

   Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

   Insert

   Cas9
   

   Use

   Tags

   Inserted T2A-miRFP670 at 3' terminal of MCS.

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSB700 HL1gR4

    Plasmid

    #124450

    Purpose

    Expresses gRNA HL1 gR4 in the pSB700 backbone

    Depositor

    George Church

    Article

    Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

    Insert

    HL1 gR4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. JN202: pMVP/SB/Blast-DEST

    Plasmid

    #121864

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. psgRNA2.02-PP7-ccdB

    Plasmid

    #99912

    Purpose

    Basic cloning vector for the expression of PP7 inserted sgRNA

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pspCas9-POLI-ST2-com vector

    Plasmid

    #176234

    Purpose

    Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AV15_pCAG.Cas9.gRNA.S1

    Plasmid

    #199259

    Purpose

    Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Inserts

    SpCas9 nuclease
    AAVS1-targeting gRNA
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter and RNA polymerase III promoter for …

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. HisMBP CFlm25

    Plasmid

    #78458

    Purpose

    Inducible expression in E. coli

    Depositor

    Sylvie Doublie

    Article

    Coseno et al Nucleic Acids Res. 2008 Jun;36(10):3474-

    Insert

    Human Cleavage Factor lm 25 kDa subunit (NUDT21 Human)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

16. psgRNA2.02-MS2-ccdB

    Plasmid

    #99911

    Purpose

    Basic cloning vector for the expression of MS2 inserted sgRNA

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SL2-sgSat-MTSb/BFP/pdCas9-C1

    Plasmid

    #162761

    Purpose

    Expressing dCas9 and sgRNA containg MTSa targeting centromeres

    Depositor

    Antony K Chen

    Article

    Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

    Insert

    dCas9 and sgRNA(SL2-sgSat-MTSb)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dCas9(nuclease deactivated Cas9)

    Promoter

    U6/CMV

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. JH401: pMVP/Ad/RGD-PK-DEST

    Plasmid

    #121846

    Purpose

    pMVP destination vector, empty RGD/PK fiber-modified adenovirus vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Adenoviral; Pmvp gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. IG301: pMVP (L1-R5) CMV promoter

    Plasmid

    #121692

    Purpose

    pMVP L1-R5 entry plasmid, contains CMV promoter for 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    CMV promoter
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HB901: pMVP (L3-L2) polyA

    Plasmid

    #121746

    Purpose

    pMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Polyadenylation signal (polyA)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge