We narrowed to 4,072 results for: p2a

1. pW267-lenti-spsgRNA-hsCDH1-sg1-pEF1s-NLS-mScarlet-I-P2A-BlastR

   Plasmid

   #170816

   Purpose

   Lentiviral vector to co-express a human CDH1 spsgRNA (sg1-Cdh1) with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

   Insert

   NLS-mScarlet-I-P2A-BlastR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

   Plasmid

   #174644

   Purpose

   Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
   

   Use

   Tags

   Venus-SSPB and iLID-mCherry

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

   Promoter

   Chicken beta-actin

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

   Plasmid

   #163643

   Purpose

   Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   pU6-CHYRON17 hgRNA
   Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
   

   Use

   Lentiviral

   Tags

   Luc2-P2A-tdTomato-T2A

   Expression

   Mutation

   The SpCas9 sgRNA constant region is mutated to ma…

   Promoter

   EFS and pU6

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9(D10A)-BPNLS-P2A-EGFP (CA77)

   Plasmid

   #208290

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpCas9(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations and V106W in TadA and nSpCas9(D10…

   Promoter

   CMV and T7

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJT259 Lenti pEF-dCas9-3XFLAG-VP64-T2A-tagBFP-P2A-Blast

   Plasmid

   #187335

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Cas9-3XFLAG-VP64-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJT216 Lenti pEF-dCas9-3XFLAG-LibCloneSite-T2A-tagBFP-P2A-Blast

   Plasmid

   #187320

   Purpose

   dCas9 expression vector with cloning site for C terminal fusions

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-EF1a-DIO-oChIEF(E163A/T199C)-P2A-dTomato-WPRE-BGHpA

   Plasmid

   #51094

   Purpose

   Cre dependent expression of oChIEF kinetic variant E163A/T199C with physically uncoupled dTomato fluorophore

   Depositor

   Jonathan Ting

   Insert

   oChIEF(E163A/T199C)
   

   Use

   AAV

   Tags

   P2A-dTomato

   Expression

   Mammalian

   Mutation

   E163A/T199C

   Promoter

   EF1a

   Available since

   May 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-EF1a-DIO-ChIEF(E162A/T198C)-P2A-dTomato-WPRE-BGHpA

   Plasmid

   #51095

   Purpose

   Cre dependent expression of ChIEF kinetic variant E162A/T198C with physically uncoupled dTomato fluorophore

   Depositor

   Jonathan Ting

   Insert

   ChIEF(E162A/T198C)
   

   Use

   AAV

   Tags

   P2A-dTomato

   Expression

   Mammalian

   Mutation

   E162A/T198C

   Promoter

   EF1a

   Available since

   May 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR

   Plasmid

   #67275

   Purpose

   For sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell lines

   Depositor

   Jannik Elverløv-Jakobsen

   Article

   Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do

   Insert

   puromycin resistant gene
   

   Use

   Tags

   linked to red flouresent protein gene (katushka)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-UCOE-SFFV-SID4x-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

    Plasmid

    #188901

    Purpose

    dCas9 with an N-term Sid4x fusion, a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    SID-dCas9-XTEN80-KRAB(Kox1)
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, P2A-GF…

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTS114 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199367

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TEV-SUMOEu-ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTS116 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199369

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TEV-SUMOstar-ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLPB3B-AID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

    Plasmid

    #187960

    Purpose

    AID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-RhoA

    Plasmid

    #165437

    Purpose

    Expresses ShadowY-CBD(H83L/H86L) and Clover(T153M/F223R)-RhoA in mammalian cells as a negative control for FLIM imaging.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-RhoA
    

    Use

    Tags

    ShadowY, Clover(T153M/F223R)

    Expression

    Mammalian

    Mutation

    H83L/H86L: CDB(Cdc42 Binding Domain, Pak3, residu…

    Promoter

    CMV

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42

    Plasmid

    #165436

    Purpose

    Expresses ShadowY-CBD(H83L/H86L) and Clover(T153M/F223R)-Cdc42 in mammalian cells as a negative control for FLIM imaging.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42
    

    Use

    Tags

    ShadowY, Clover(T153M/F223R)

    Expression

    Mammalian

    Mutation

    H83L/H86L: CDB(Cdc42 Binding Domain, Pak3, residu…

    Promoter

    CMV

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF1a_BbsI-gRNA scaffold-EC73-5'-EcoRI-EC73-3'-HDV_pA_pCMV_EGFP-L202S-P2A-mCherry_pA

    Plasmid

    #176461

    Purpose

    Vector for constructing retron Ec73ncRNA-gRNA chimeric RNA (Ec73 rgRNA), inserting spacer sequence by BbsI digestion and inserting donor sequence by EcoRI digestion. EGFP(L202S)-P2A-mCherry driven by CMV

    Depositor

    Hui Yang

    Article

    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

    Insert

    BbsI-gRNA scaffold-Ec73ncRNA-EcoRI-HDV
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV EF1A mSOG1-94-FRB-P2A-T2A-FKBP-mSOG-Ja95-140

    Plasmid

    #136601

    Purpose

    Lentiviral vector encoding mSOG1-94-FRB-P2A-T2A-FKBP-mSOG-Ja95-140 under the EF1A promoter.

    Depositor

    John Ngo

    Article

    Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

    Insert

    N-mSOG-FRB-P2A-T2A-FKBP-C-mSOG
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHIE050 ZF43x0 mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138731

    Purpose

    Integration Vector, assembled with pHIE042 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x0
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHIE053 ZF43x6-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138734

    Purpose

    Integration Vector, assembled with pHIE045 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x6-Compact
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLPB3B-mAID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

    Plasmid

    #187963

    Purpose

    mAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Inserts

    AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
    osTIR1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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