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Plasmid#180692PurposeAssembly cassette with ConLS and ConR1 connectors to be used in the Dueber YTK and Ellis MYT systemsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only
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pTU1-D-yckABD
Plasmid#112790PurposeDownstream homology arm, Pair of HAs spans yckA and yckB, which share homology with ABC transporters in B. subtilisDepositorArticleInsertyckABD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-yckABU
Plasmid#112789PurposeUpstream homology arm, Pair of HAs spans yckA and yckB, which share homology with ABC transporters in B. subtilisDepositorArticleInsertyckABU
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU2-AmyE-Pveg-eGFP
Plasmid#112792PurposeExpresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformationDepositorArticleInsertPveg-eGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW019-2
Plasmid#85614PurposeInducible dCas9 integration vector for Bacillus subtilisDepositorInsertdcas9
UseTagsExpressionBacterialMutationPromoterPxylA (B. megaterium)Available sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-knt-P-R*
Plasmid#189852PurposeCre-inducible activation of knt for kanamycin resistance.DepositorInsertP-loxP-TT-loxP-R*-knt
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterP (T7 phage gene 10)Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAW016-2
Plasmid#85613PurposeCas9-tracrRNA integration vector for Bacillus subtilisDepositorInsertcas9-tracrRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAW014-2
Plasmid#85612PurposeMulti-gRNA delivery vector containing ugtP-gRNA.P395T for Bacillus subtilisDepositorInsertugtP-gRNA.P395T
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-knt-P*-R
Plasmid#189850PurposeCre-inducible activation of knt for kanamycin resistance.DepositorInsertP*-loxP-TT-loxP-R-knt
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterP* (modified T7 phage gene 10)Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-knt-P-R
Plasmid#189849PurposeCre-inducible activation of knt for kanamycin resistance.DepositorInsertP-loxP-TT-loxP-R-knt
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterP (T7 phage gene 10)Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-knt-P**-R
Plasmid#189851PurposeCre-inducible activation of knt for kanamycin resistance.DepositorInsertP**-loxP-TT-loxP-R-knt
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterP** (modified T7 phage gene 10)Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-cat-P-R
Plasmid#189853PurposeCre-inducible activation of cat for chloramphenicol resistance.DepositorInsertP-loxP-TT-loxP-R-cat
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterP (T7 phage gene 10)Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYHY1
Plasmid#215926PurposeE. coli-Bacteroides shuttle vector.DepositorTypeEmpty backboneUseSynthetic Biology; E. coli-bacteroides shuttle ve…TagsExpressionBacterialMutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMYT017
Plasmid#180670PurposeEncodes LacI as a Type 3 part to be used in the Dueber YTK and Ellis MYT systemsDepositorInsertLacI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFH114
Plasmid#128408PurposeLevel1 ZmUBIp::BAR:NOStDepositorInsertLevel1 ZmUBIp::BAR:NOSt (Position 1)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
EC67907
Plasmid#211794PurposeOver expresses Cas9 and GR-GRF-GIF. Must receive guide cassettesDepositorInsertHptII, Cas9, GR-GRF-GIF
UseTagsExpressionPlantMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
TtRMPVIR
Plasmid#27995DepositorInsertTtRMPVIR (shRen)
UseRNAi and Retroviral; Fluorescent reportersTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMYT018
Plasmid#180671PurposeEncodes rtTA as a Type 3 part to be used in the Dueber YTK and Ellis MYT systemsDepositorInsertrtTA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT016
Plasmid#180669PurposeEncodes Z3EV as a Type 3 part to be used in the Dueber YTK and Ellis MYT systemsDepositorInsertZ3EV
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT015
Plasmid#180668PurposeEncodes pTet7 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systemsDepositorInsertpTet7
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only