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pTol1 - 14xUAS:CaSR-EGFP

Plasmid

#191502

Purpose

A UAS construct used to create transgenic fish that express CaSR-EGFP when crossed to a fish carrying a Gal4.

Depositor

Michael Granato

Publication

Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

Insert

CaSR-EGFP-SV40

Use

Creation of transgenic fish using tol1 transgenes…

Promoter

14X UAS

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA ins:R198P mVenus

Plasmid

#210519

Purpose

Expresses mVenus-tagged R198P mutant of Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoter

Depositor

Jonathan Rocheleau

Publication

Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

Insert

Apollo-NADP+

Use

Zebrafish expression, tol2 kit gateway-compatible

Tags

mVenus

Mutation

R198P

Promoter

Zebrafish insulin promoter

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA ins:Apollo-NADP+ mVenus

Plasmid

#210518

Purpose

Expresses mVenus-tagged Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoter

Depositor

Jonathan Rocheleau

Publication

Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

Insert

Apollo-NADP+

Use

Zebrafish expression, tol2 kit gateway-compatible

Tags

mVenus

Promoter

Zebrafish insulin promoter

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCK005 ubi:mCerulean_ubb-polyA

Plasmid

#195979

Purpose

Expression construct

Depositor

Alexa Burger, Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

ubi:mCerulean

Use

Other

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pT22UbGfpnE4PCh(#301)

Plasmid

#184066

Purpose

cyclofen-inducible GFP nuclear relocation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Publication

Zhang et al Chembiochem. 2018 Jun 18;19(12):1232-123

Insert

eGFP-nls-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcryGFP-acta1-dmdGFP

Plasmid

#172530

Purpose

alphaA-crystallin promoter (cry) drives GFP expression in the lens. The acta1 muscle-specific promoter drives expression of GFP-tagged zebrafish dmd.

Depositor

Joachim Berger

Publication

Berger et al J Cell Mol Med. 2020 Jun;24(12):6680-668

Insert

dmd (dmd Zebrafish)

Tags

GFP

Expression

Mammalian

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-Hs.KCNJ13-IRES-EGFP

Plasmid

#164945

Purpose

Tol2 construct: actinb (actb2) promoter driven human KCNJ13 and EGFP”. This will reflect the linkage of KCNJ13 and EGFP

Depositor

GuangJun Zhang

Publication

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

KCNJ13 (KCNJ13 Human)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Mutation

Stop codon deleted

Promoter

actinb (actb2) promoter

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pME-QUASM1:GFPNLS-SV40pA

Plasmid

#155116

Purpose

Middle entry vector containing QUASM1 element upstream of GFP-NLS.

Depositor

Bret Pearson

Publication

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:GFPNLS

Use

Gateway middle entry vector

Promoter

QUASM1-E1b

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pTK-ENH_BsmB1-MCS-Avi

Plasmid

#110205

Purpose

Recipient vector for expression of C-terminally Avi-tagged protein of interest in chick embryos under the control of enhancer of choice

Depositor

Tatjana Sauka-Spengler

Publication

Lukoseviciute et al STAR Protoc. 2020 Jul 31;1(2):100066. do

Insert

ENH_BsmB1-MCS-Avi

Tags

Avi_tag

Expression

Bacterial

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pTK-ENH_BsmB1-Avi-MCS-2A-Citrine

Plasmid

#110204

Purpose

Recipient vector for expression of N-terminally Avi-tagged protein of interest in chick embryos under the control of enhancer of choice.

Depositor

Tatjana Sauka-Spengler

Publication

Lukoseviciute et al STAR Protoc. 2020 Jul 31;1(2):100066. do

Insert

ENH_BsmB1-Avi-MCS-2A-Citrine

Tags

Avi_tag-2a-YFP

Expression

Bacterial

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX1ZFCNGA5

Plasmid

#69859

Purpose

GST tagged protein for antibody production screening

Depositor

Gary Matthews

Publication

Khan et al Neuroscience. 2010 Jan 13;165(1):79-89.

Insert

ZFCNGA5, 3' end only with native stop (cnga5 Zebrafish)

Tags

GST

Expression

Bacterial

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-human lyn(C468A)–GFP

Plasmid

#35959

Purpose

Expresses human lyn protein codon optimized for zebrafish expression.

Depositor

Anna Huttenlocher

Publication

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LYN (LYN Human)

Tags

EGFP

Expression

Mammalian

Mutation

c468a

Promoter

cmv

Available since

April 27, 2012

Availability

Academic Institutions and Nonprofits only
pCS2+ EGFP-3NLS

Plasmid

#165400

Purpose

Expresses nuclear localized EGFP

Depositor

Philipp Niethammer

Publication

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nuclear Localization Signal

Use

Avian, xenopus, zebrafish

Tags

EGFP

Expression

Mammalian

Promoter

SP6

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKSII/INSeGFP

Plasmid

#53787

Purpose

1 kb zebrafish insulin promoter driving eGFP

Depositor

Larry Moss

Publication

diIorio et al Dev Biol. 2002 Apr 1;244(1):75-84.

Insert

INS promoter/eGFP/pA

Use

Zebrafish

Tags

eGFP

Expression

Bacterial

Promoter

zebrafish insulin/ZIP

Available since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only
pET28b-DiCas7-11-His

Plasmid

#234482

Purpose

Plasmid for bacterial expression and purification of DiCas7-11 protein (human codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Publication

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

DiCas7-11

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Available since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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p17xQUAS-Luciferase, α-cry:mCherry

Plasmid

#180482

Purpose

Inducible gene expression vector p17xQUAS-Luciferase, α-cry:mCherry

Depositor

Hyunju Ro

Publication

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Luciferase
17x QUAS

Use

Synthetic Biology

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-GGGS

Plasmid

#162610

Purpose

Allows for transcription of control mVenus fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Publication

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mVenus

Expression

Mammalian

Mutation

Contains aa1-155 of mVenus

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-GGGS-TDP-43

Plasmid

#162611

Purpose

Allows for transcription of mVenus fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Publication

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TARDBP (TARDBP Human)

Expression

Mammalian

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_gpN:SnoopTag

Plasmid

#225195

Purpose

Used for adding C-terminal SnoopTag to the E. coli HS P2 phage major capsid protein

Depositor

Travis Wiles

Publication

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SnoopTag

Expression

Bacterial

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_gpN:SpyTag003

Plasmid

#225194

Purpose

Used for adding C-terminal SpyTag003 to the E. coli HS P2 phage major capsid protein

Depositor

Travis Wiles

Publication

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag003

Expression

Bacterial

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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