We narrowed to 19,779 results for: INO

Showing: 2261 - 2280 of 19779 results

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pCDF-casBCDE(g8)

Plasmid

#89727

Purpose

Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing 33-nt spacer (referred to as 'wt') matching a protospacer in the M13 phage genome.

Depositor

Konstantin Kuznedelov, Konstantin Severinov

Article

Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

CRISPR array

Expression

Bacterial

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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Kv7.4 (1-645, Δ368-523)/HA tag/pcDNA3.1

Plasmid

#111459

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

Ha Tag

Expression

Mammalian

Mutation

Kv7.4 (1-645, Δ368-523), C643A

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pT2KXIG (Tol2)-tp63:ikk1C179A:GFP

Plasmid

#105647

Purpose

Expression of Ikk1(alpha) with mutated cysteine residues at position 179 in basal keratinocytes

Depositor

Sandra Rieger

Article

Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

Insert

tp63:ikk1C179A:GFP (chuk Zebrafish)

Tags

GFP

Expression

Mammalian

Mutation

p63 promoter + ikk1C179A + GFP

Available Since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pIRES:mTREK-1(K271Q)

Plasmid

#133273

Purpose

Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght K271Q mutant fused to a N-terminal HA tag

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)

Tags

HA

Expression

Mammalian and Yeast

Mutation

K271Q

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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Kv7.4 AB (325-557, Δ 368-523)/pET28HMT

Plasmid

#111052

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 AB (325-557, Δ 368-523)

Promoter

T7

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCELF PAI-RBP v3

Plasmid

#45507

Depositor

Thomas Gelehrter

Article

Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

Insert

PAI-RBP v3 (SERBP1 Rat, Human)

Tags

GST tag

Expression

Mammalian

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
Rb-379-928Δ703-737-pGex2T

Plasmid

#21933

Depositor

William Kaelin

Article

Kaelin et al Cell. 1991 Feb 8. 64(3):521-32.

Insert

Rb (RB1 Human)

Tags

GST

Expression

Bacterial

Mutation

deletion mutant ORF contains aa 379-928D703-737 o…

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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mChF-AIP (TD)

Plasmid

#61529

Purpose

Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP (with TD mutation)

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

FK506 binding protein 1A (FKBP1A Human)

Tags

AIP with TD mutation (see comments) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only
mChF-AIP (TE)

Plasmid

#61530

Purpose

Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP (with TE mutation)

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

FK506 binding protein 1A (FKBP1A Human)

Tags

AIP with TE mutation (see comments) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
FLAG-Cerulean-UCR1

Plasmid

#67578

Purpose

Expresses the UCR1 region of PDE4D3 in mammalian cells

Depositor

Graeme Carnegie

Article

Wang et al Cell Signal. 2015 May;27(5):908-22. doi:

Insert

Upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)

Tags

Cerulean and FLAG

Expression

Mammalian

Mutation

Amino acid residues 17-136 of PDE4D3

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only
Rb-379-928Δ738-775-pGex2T

Plasmid

#21783

Depositor

William Kaelin

Article

Kaelin et al Cell. 1991 Feb 8. 64(3):521-32.

Insert

Rb (RB1 Human)

Tags

GST

Expression

Bacterial

Mutation

deletion mutant ORF contains aa 379-928D737-774 o…

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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pCR8-CDK12

Plasmid

#208347

Purpose

Gateway cloning entry vector with CDK12. Must be recombined into a DEST vector for expression.

Depositor

Peter S Nelson

Article

Frank et al eLife 2025

Insert

cyclin dependent kinase 12 (CDK12 Human)

Use

Gateway: entry vector

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-KRAS

Plasmid

#191134

Purpose

expresses KRAS in mammalian cells

Depositor

Ken-ichirou Morohashi

Article

Inoue et al Commun Biol. 2022 Sep 15;5(1):974. doi:

Insert

Kirsten rat sarcoma viral oncogene (Kras Mouse)

Expression

Mammalian

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCAD123_hIrg1_N152K,M154I_pvp008

Plasmid

#239118

Purpose

hACOD1 with N152K and M154I mutations in E.coli expression vector

Depositor

Konrad Buessow

Article

Chen et al Sci Rep. 2023 Jun 26;13(1):10360. doi: 1

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

Deleted amino acids 1-3 and 462-end. Mutated N152…

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mCGN-mCGNL1-Rod1-Myc

Plasmid

#236526

Purpose

For expression of chimeric CGN containing the Rod1 domain from CGNL1 (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Flinois et al J Cell Sci. 2024 Mar 1;137(5):jcs260745.

Insert

CGN-CGNL1 (Cgn Mouse)

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1(tandem S131C-wt)

Plasmid

#224777

Purpose

Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (S131C-wt) (Kcnk2 Mouse)

Expression

Mammalian

Mutation

S131C

Promoter

CMV

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1(S131C/A286F)

Plasmid

#224775

Purpose

Mammalian expression vector for generating mouse TREK-1

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Expression

Mammalian

Mutation

S131C / A286F

Promoter

CMV

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1(tandem wt-S131C)

Plasmid

#224778

Purpose

Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 wt-S131C) (Kcnk2 Mouse)

Expression

Mammalian

Mutation

S562C

Promoter

CMV

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1(tandem wt-wt)

Plasmid

#224776

Purpose

Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (wt-wt) (Kcnk2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(tandem S131C-S131C)

Plasmid

#224760

Purpose

In vitro transcription for Oocyte expression of mTREK-1(tandem S131C-S131C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (S131C-S131C) (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131C / S562C

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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