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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,620 results for: Msc

Showing: 2261 - 2280 of 2620 results

  2. MO91-dCas9-AID(P182X)

    Plasmid
    #83260
    Purpose
    Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone.
    Depositor
    Xing Chang
    Article
    Ma et al Nat Methods. 2016 Dec;13(12):1029-1035.
    Insert
    dCas9-AID P182X
    Use
    Retroviral
    Tags
    3xFLAG-NLS and FLAG
    Expression
    Mammalian
    Mutation
    D10A and H840A in Cas9; P182X in AID
    Promoter
    MSCV
    Available Since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only

  6. eSpCas9(1.1)_No_FLAG_LMNA_G2

    Plasmid
    #178090
    Purpose
    Expresses the LMNA G2 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with LMNA_mScarlet-I_Donor to tag LMNA with mScarlet-I. pX330-like plasmid.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    LMNA G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178090-plasmid-map-sequence-id-350140

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  7. MIGR1-cyclin E-AA

    Plasmid
    #47498
    Purpose
    MSCV-based vector encodes human cyclin E mutated within N- and C-terminal Cdc4 phosphodegrons
    Depositor
    Alex Minella
    Article
    Xu et al Oncogene. 2013 Jul 22. doi: 10.1038/onc.
    Insert
    cyclin E1 (CCNE1 Human)
    Use
    Retroviral; Mscv-ires-gfp
    Tags
    Expression
    Mammalian
    Mutation
    threonines 62 and 380 to alanine (T62A T380A)
    Promoter
    LTR
    Available Since
    Sept. 9, 2013
    Availability
    Academic Institutions and Nonprofits only

  10. L2085-WT-GPA-NLuc-Biosensor-Retrovirus-TD135

    Plasmid
    #222875
    Purpose
    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). WT Glycophorin A (GPA) scaffold.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA(WT)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    MSCV LTR
    Available Since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    222875-plasmid-map-sequence-id-441838

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  11. pAN1646

    Plasmid
    #127205
    Purpose
    pTet expression of Venus, Dual inducible expression of dCas9-VP64-mScarlet-degronSwitchA and KeyA-BFP-NLS
    Depositor
    Hana El-Samad
    Article
    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586
    Insert
    p7x_tet-Venus-tADH1-pGal1-dCas9-VP64-Linker-mScarlet-Linker-degronSwitch_A_t12-tPGK1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tSSA1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Availability
    Academic Institutions and Nonprofits only
    127205-plasmid-map-sequence-id-250026

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  14. pCSIIpuro-TGFa-ScNeo

    Plasmid
    #209895
    Purpose
    To monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    TGFa-ScNeo (TGFA Human)
    Use
    Lentiviral
    Tags
    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…
    Expression
    Mutation
    Promoter
    Available Since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209895-plasmid-map-sequence-id-416585

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  17. pCSIIpuro-Necl5-ScNeo

    Plasmid
    #209901
    Purpose
    To monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    Necl5-ScNeo (Pvr Mouse)
    Use
    Lentiviral
    Tags
    mNeonGreen and mScarlet
    Expression
    Mutation
    Promoter
    Available Since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209901-plasmid-map-sequence-id-416655

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  19. pPBbleo-TGFa-ScNeo

    Plasmid
    #209904
    Purpose
    To monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    TGFa-ScNeo (TGFA Human)
    Use
    Tags
    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209904-plasmid-map-sequence-id-421035

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  20. pPBbsr2-TGFa-ScNeo

    Plasmid
    #209908
    Purpose
    To monitor the status of TGFα, the plasmid encodes a recombinant TGFα, fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    TGFa-ScNeo (TGFA Human)
    Use
    Tags
    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209908-plasmid-map-sequence-id-416691

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Showing: 2261 - 2280 of 2620 results