We narrowed to 5,822 results for: pCas

1. pSPcas9(BB)-2A-Puro V2.0 sgCHMP2B-2 aauucccaaaugaagauggc

   Plasmid

   #231998

   Purpose

   Expression of an sgRNA targeting CHMP2B, Cas9, and a PuroR marker

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   CHMP2B-targeted sgRNA (CHMP2B Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-pCAG-iCRE-2A-H2BGFP-WPRE

   Plasmid

   #234893

   Purpose

   CAG-driven Cre recombinase coupled with H2B-GFP for nuclear fluorescent labelling

   Depositor

   Silvia Arber

   Article

   Falasconi et al Nature. 2025 May 28. doi: 10.1038/s41586

   Insert

   iCRE-2A-H2BGFP
   

   Use

   AAV

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCBh-T7-SpCas9-P2A-mTagBFP2(RAS3575)

   Plasmid

   #223111

   Purpose

   pCBh Human expression plasmid for SpCas9 with with C-term BPNLS-P2A-mTagBFP2

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9-P2A-mTagBFP2
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-mTagBFP2

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-MTS(S17/43A)-TFAM-V5

   Plasmid

   #202404

   Purpose

   expresses MTS(S17/43A)-TFAM-V5 in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   Transcription Factor A, Mitochondrial (TFAM Human)
   

   Tags

   V5

   Expression

   Mammalian

   Mutation

   S17A, S43A

   Promoter

   CAG

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-MTS(S17/43A)-EGFP-V5

   Plasmid

   #200935

   Purpose

   expresses MTS(S17/43A)-EGFP-V5 in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   EGFP
   

   Tags

   V5 and mitochondrial targeting sequence (MTS)

   Expression

   Mammalian

   Mutation

   S17A, S43A in MTS

   Promoter

   CAG

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-GPHN-FingR-mGold-CCR5TC

   Plasmid

   #231784

   Purpose

   Visualization of inhibitory post-synapse using GPHN-FingR-mGold

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   GPHN-FingR-mGold-CCR5TC
   

   Expression

   Mammalian

   Promoter

   Actin promoter with CMV enhancer

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-GPHN-FingR-mVenus-CCR5TC

   Plasmid

   #231783

   Purpose

   Visualization of inhibitory post-synapse using GPHN-FingR-mVenus

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   GPHN-FingR-mVenus-CCR5TC
   

   Expression

   Mammalian

   Promoter

   Actin promoter with CMV enhancer

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-GPHN-FingR-mGold2t-CCR5TC

   Plasmid

   #231786

   Purpose

   Visualization of inhibitory post-synapse using GPHN-FingR-mGold2t

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   GPHN-FingR-mGold2t-CCR5TC
   

   Expression

   Mammalian

   Promoter

   Actin promoter with CMV enhancer

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS puro human RLIM N581K

   Plasmid

   #232242

   Purpose

   Mammalian expression of Human RLIM N581K

   Depositor

   Francisco Bustos

   Article

   Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

   Insert

   Human RLIM N581K (RLIM Human)
   

   Expression

   Mammalian

   Mutation

   N581K

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-Twin-strep-TEV-DGKδ2

    Plasmid

    #223720

    Purpose

    Express human diacylglycerol kinase delta 2 protein (N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

    Depositor

    Chiaki Murakami

    Insert

    DGKD (DGKD Human)
    

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    deleted start codon

    Promoter

    CAG and chicken β-actin promoter

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-PHOSPHO2-TEV-Twin-Strep

    Plasmid

    #222016

    Purpose

    Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

    Depositor

    Chiaki Murakami

    Insert

    PHOSPHO2 (PHOSPHO2 Human)
    

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    deleted stop codon (TAA)

    Promoter

    chicken β-actin promoter

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-PHOSPHO1-TEV-Twin-Strep

    Plasmid

    #222015

    Purpose

    Express human PHOSPHO1 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

    Depositor

    Chiaki Murakami

    Insert

    PHOSPHO1 (PHOSPHO1, PHOSPHO1 Human)
    

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    stop codon (TGA) is deleted

    Promoter

    chicken β-actin promoter

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-U6-BRD4-HaloTag-Guide-hspCas9

    Plasmid

    #228576

    Purpose

    Expression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertion

    Depositor

    Marcin Imielinski

    Insert

    BRD4 gRNA (BRD4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #223323

    Purpose

    Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

    Depositor

    Jonathan Arias

    Article

    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

    Insert

    SpCas9 and sgRNA-T2 (cas9 )
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCA528 IST1 L328D, L355A (1-366)

    Plasmid

    #193040

    Purpose

    Bacterial expression plasmid for full-length IST1 (1-366) with L328D and L355A mutations that diminish binding to MIT domains.

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    L328D, L355A

    Promoter

    T7

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG CAPN7 V18D,F98D-OSF

    Plasmid

    #193045

    Purpose

    Mammalian expression plasmid encoding full-length CAPN7 with V18D and F98D mutations that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Tags

    OSF (One-Strep-FLAG)

    Expression

    Mammalian

    Mutation

    V18D, F98D

    Promoter

    Chicken Beta-actin

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCA528 CAPN7 V18D,F98D (1-165)

    Plasmid

    #193034

    Purpose

    Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with V18D and F98D mutations that abolish binding to IST1.

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    V18D, F98D, Residues 1-165

    Promoter

    T7

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

    Plasmid

    #221552

    Purpose

    Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human Rab7A (RAB7A Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJS-BCD-Strep-PCaspase-R153A

    Plasmid

    #214036

    Purpose

    Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) R153A mutant from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    PCaspase
    

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    R153A

    Promoter

    T7 promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS-3xFLAG-bII spectrin (repeats 13-15)

    Plasmid

    #213729

    Purpose

    Mammalian cell expression vector for overexpression of bII spectrin (repeats 13-15) in NIH/3T3 cells.

    Depositor

    Chaolin Zhang

    Article

    Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

    Insert

    bII spectrin (repeats 13-15) (Spnb3 Mouse)
    

    Expression

    Mammalian

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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