We narrowed to 6,923 results for: pcdna3.1

Showing: 2261 - 2280 of 6923 results

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opto-Cdc42

Plasmid

#174510

Purpose

Expresses opto-Cdc42 (mCherry-GGGSx2-Cdc42-GGGSx2-BcLOV4-GGGS-3xFLAG) in a pcDNA3.1 backbone.

Depositor

Brian Chow

Publication

Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

Insert

opto-Cdc42-mCherry

Tags

3xFLAG and mCherry

Expression

Mammalian

Promoter

CMV

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pYU-2-Env

Plasmid

#133997

Purpose

Env gene from HIV-1 YU-2 in pcDNA3.1 expression vector

Depositor

Himanshu Garg

Publication

Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

Insert

Envelope Glycoprotein

Expression

Mammalian

Promoter

CMV

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP-Keap1 (mouse)

Plasmid

#236421

Purpose

transient overexpression of KEAP1 in mammalian cells

Depositor

Michele Pagano

Publication

Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

Insert

KEAP1 (Keap1 Mouse)

Expression

Mammalian

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-iPASnFR-L-mScarlet3-3xFLAG-Rab5

Plasmid

#225244

Purpose

Mammalian expression of iPASnFR-L fused to Rab5

Depositor

Loren Looger

Publication

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-L

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-iPASnFR-H-mScarlet3-3xFLAG-Rab5

Plasmid

#225243

Purpose

Mammalian expression of iPASnFR-H fused to Rab5

Depositor

Loren Looger

Publication

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-H

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP Rab11a S25N pcDNA3.1 Zeo (+)

Plasmid

#206080

Purpose

dominant-negative Rab11a mutant - S25N mutation causes inhibition of Rab11a-dependent recycling

Depositor

Annette Dolphin

Publication

Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

Insert

RAB11A (RAB11A Human)

Tags

EGFP

Expression

Mammalian

Mutation

S25N

Promoter

CMV

Available since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 GIL-11 myc TEV TwinStrep

Plasmid

#199627

Purpose

Eucaryotic expression plasmid for chimeric cytokine of IL-11 and LIF (GIL-11) with a TwinStrep-tag

Depositor

Jurgen Scheller

Publication

Rafii et al Commun Biol. 2023 Apr 15;6(1):418. doi:

Insert

GIL-11

Tags

TwinStrep-tag

Expression

Mammalian

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (+) -myc-OGA(GS-544)C181

Plasmid

#193996

Purpose

plasmid encoding myc-tagged OGA(GS-544)C181

Depositor

Christina Woo

Publication

Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

Insert

myc-OGA(GS-544)C181

Tags

myc

Expression

Mammalian

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-IL2sp-6His-Asprosin plasmid

Plasmid

#190683

Purpose

Expresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells.

Depositor

Atul Chopra

Publication

Mishra et al STAR Protoc. 2022 Dec 16;3(4):101762. do

Insert

IL2-6xHis-Asprosin

Tags

IL2 signal peptide, 6xHis

Expression

Mammalian

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-DnaJB1-PKAcat(K72H)-mCherry

Plasmid

#181851

Purpose

mCherry-tagged DnaJB1-PKA catalytic subunit fusion; contains catalytically dead PKA; for mammalian expression.

Depositor

Jin Zhang

Publication

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

DnaJB1-PKAcat(K72H)-mCherry (PRKACA, DNAJB1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Lysine 128 in the DnaJB1-PKAcat fusion changed to…

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-CIBN-mCherry-VAMP2

Plasmid

#178573

Purpose

Mammalian expression plasmid of CIBN-VAMP2 of Opto-vTrap

Depositor

Won Do Heo

Publication

Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

Insert

CIBN-mCherry-VAMP2

Expression

Mammalian

Promoter

CMV

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD81-(TEV)-FKBP-Myc

Plasmid

#177908

Purpose

human CD81-TEV cleavage site-FKBP-Myc tag

Depositor

Masaharu Somiya

Publication

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

human CD81 (CD81 Synthetic, Human)

Tags

FKBP, Myc tag, and TEV protease cleavage site

Expression

Mammalian

Promoter

CMV

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-hH3-(GGS)X6-HaloTag C58

Plasmid

#171432

Purpose

Expression of hH3-(GGS)X6-HaloTag C58 in mammalian cells

Depositor

Yujie Sun

Publication

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

HaloTag N58-(GGS)X6-hH3

Expression

Mammalian

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CCND1(T286A)

Plasmid

#172647

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D1(T286A) in mammalian cells

Depositor

Michele Pagano

Publication

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Thr286Ala

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ecDHFR(DD)-MS2CP-VPg(FCV)

Plasmid

#167313

Purpose

Template pDNA to prepare DD-CaVT mRNA

Depositor

Hirohide Saito

Publication

Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

Insert

DD-CaVT

Use

Synthetic Biology

Tags

DYK-tag

Expression

Mammalian

Promoter

CMV

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1-tvD-Cit-tevs-DHFR

Plasmid

#116054

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Publication

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

tvD-Cit-tevs-DHFR

Expression

Mammalian

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-rnSOX9 PQA/S (286-507)

Plasmid

#62975

Purpose

Expresses HA-tagged rat SOX9 residues 286-507

Depositor

Michael Ruppert

Publication

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

SOX9 (Sox9 Rat)

Tags

HA (YPYDVPDY)

Expression

Mammalian

Mutation

codes for residues 286-507

Promoter

CMV

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 myc-mβTrCP F-box (1-247)

Plasmid

#62976

Purpose

Expresses myc-tagged mouse βTrCP residues 1-247

Depositor

Michael Ruppert

Publication

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

BTRC (Btrc Mouse)

Tags

3x myc

Expression

Mammalian

Mutation

codes for residues 1-247

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
Cry2 mCherry RGS4(del 1-33) in pcDNA3.1

Plasmid

#64207

Purpose

mCherry fused between Cry2 and RGS4(del 1-33) to study cell migration

Depositor

Narasimhan Gautam

Publication

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 (CRY2 Mustard Weed)
RGS4(del 1-33) (RGS4 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
NC4 pCDNA3.1 VENUS WTX (1-804)

Plasmid

#36959

Depositor

Randall Moon

Publication

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

WTX (AMER1 Human)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available since

July 24, 2012

Availability

Academic Institutions and Nonprofits only